Temporal Gene Expression Kinetics for Human Keratinocytes Exposed to Hyperthermic Stress
The gene expression kinetics for human cells exposed to hyperthermic stress are not well characterized. In this study, we identified and characterized the genes that are differentially expressed in human epidermal keratinocyte (HEK) cells exposed to hyperthermic stress. In order to obtain temporal g...
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-04-01
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| Series: | Cells |
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| Online Access: | http://www.mdpi.com/2073-4409/2/2/224 |
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| author | Gerald J. Wilmink Caleb C. Roth Ibtissam Echchgadda Cesario Z. Cerna |
| author_facet | Gerald J. Wilmink Caleb C. Roth Ibtissam Echchgadda Cesario Z. Cerna |
| author_sort | Gerald J. Wilmink |
| collection | DOAJ |
| description | The gene expression kinetics for human cells exposed to hyperthermic stress are not well characterized. In this study, we identified and characterized the genes that are differentially expressed in human epidermal keratinocyte (HEK) cells exposed to hyperthermic stress. In order to obtain temporal gene expression kinetics, we exposed HEK cells to a heat stress protocol (44 °C for 40 min) and used messenger RNA (mRNA) microarrays at 0 h, 4 h and 24 h post-exposure. Bioinformatics software was employed to characterize the chief biological processes and canonical pathways associated with these heat stress genes. The data shows that the genes encoding for heat shock proteins (HSPs) that function to prevent further protein denaturation and aggregation, such as HSP40, HSP70 and HSP105, exhibit maximal expression immediately after exposure to hyperthermic stress. In contrast, the smaller HSPs, such as HSP10 and HSP27, which function in mitochondrial protein biogenesis and cellular adaptation, exhibit maximal expression during the “recovery phase”, roughly 24 h post-exposure. These data suggest that the temporal expression kinetics for each particular HSP appears to correlate with the cellular function that is required at each time point. In summary, these data provide additional insight regarding the expression kinetics of genes that are triggered in HEK cells exposed to hyperthermic stress. |
| first_indexed | 2024-03-12T10:49:50Z |
| format | Article |
| id | doaj.art-b2f2a23f8608438793d27571725d7fdc |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T10:49:50Z |
| publishDate | 2013-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-b2f2a23f8608438793d27571725d7fdc2023-09-02T07:12:26ZengMDPI AGCells2073-44092013-04-012222424310.3390/cells2020224Temporal Gene Expression Kinetics for Human Keratinocytes Exposed to Hyperthermic StressGerald J. WilminkCaleb C. RothIbtissam EchchgaddaCesario Z. CernaThe gene expression kinetics for human cells exposed to hyperthermic stress are not well characterized. In this study, we identified and characterized the genes that are differentially expressed in human epidermal keratinocyte (HEK) cells exposed to hyperthermic stress. In order to obtain temporal gene expression kinetics, we exposed HEK cells to a heat stress protocol (44 °C for 40 min) and used messenger RNA (mRNA) microarrays at 0 h, 4 h and 24 h post-exposure. Bioinformatics software was employed to characterize the chief biological processes and canonical pathways associated with these heat stress genes. The data shows that the genes encoding for heat shock proteins (HSPs) that function to prevent further protein denaturation and aggregation, such as HSP40, HSP70 and HSP105, exhibit maximal expression immediately after exposure to hyperthermic stress. In contrast, the smaller HSPs, such as HSP10 and HSP27, which function in mitochondrial protein biogenesis and cellular adaptation, exhibit maximal expression during the “recovery phase”, roughly 24 h post-exposure. These data suggest that the temporal expression kinetics for each particular HSP appears to correlate with the cellular function that is required at each time point. In summary, these data provide additional insight regarding the expression kinetics of genes that are triggered in HEK cells exposed to hyperthermic stress.http://www.mdpi.com/2073-4409/2/2/224keratinocytesheat shockgene expressioncellular stress responsebioinformatics |
| spellingShingle | Gerald J. Wilmink Caleb C. Roth Ibtissam Echchgadda Cesario Z. Cerna Temporal Gene Expression Kinetics for Human Keratinocytes Exposed to Hyperthermic Stress Cells keratinocytes heat shock gene expression cellular stress response bioinformatics |
| title | Temporal Gene Expression Kinetics for Human Keratinocytes Exposed to Hyperthermic Stress |
| title_full | Temporal Gene Expression Kinetics for Human Keratinocytes Exposed to Hyperthermic Stress |
| title_fullStr | Temporal Gene Expression Kinetics for Human Keratinocytes Exposed to Hyperthermic Stress |
| title_full_unstemmed | Temporal Gene Expression Kinetics for Human Keratinocytes Exposed to Hyperthermic Stress |
| title_short | Temporal Gene Expression Kinetics for Human Keratinocytes Exposed to Hyperthermic Stress |
| title_sort | temporal gene expression kinetics for human keratinocytes exposed to hyperthermic stress |
| topic | keratinocytes heat shock gene expression cellular stress response bioinformatics |
| url | http://www.mdpi.com/2073-4409/2/2/224 |
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