An Ultra-Rapid Biosensory Point-of-Care (POC) Assay for Prostate-Specific Antigen (PSA) Detection in Human Serum
Prostate-specific antigen (PSA) is the established routine screening tool for the detection of early-stage prostate cancer. Given the laboratory-centric nature of the process, the development of a portable, ultra rapid high-throughput system for PSA screening is highly desirable. In this study, an a...
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| Format: | Article |
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MDPI AG
2018-11-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/18/11/3834 |
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| author | Sophie Mavrikou Georgia Moschopoulou Athanasios Zafeirakis Konstantina Kalogeropoulou Georgios Giannakos Athanasios Skevis Spyridon Kintzios |
| author_facet | Sophie Mavrikou Georgia Moschopoulou Athanasios Zafeirakis Konstantina Kalogeropoulou Georgios Giannakos Athanasios Skevis Spyridon Kintzios |
| author_sort | Sophie Mavrikou |
| collection | DOAJ |
| description | Prostate-specific antigen (PSA) is the established routine screening tool for the detection of early-stage prostate cancer. Given the laboratory-centric nature of the process, the development of a portable, ultra rapid high-throughput system for PSA screening is highly desirable. In this study, an advancedpoint-of-care system for PSA detection in human serum was developed based on a cellular biosensor where the cell membrane was modified by electroinserting a specific antibody against PSA. Thirty nine human serum samples were used for validation of this biosensory system for PSA detection. Samples were analyzed in parallel with a standard immunoradiometric assay (IRMA) and an established electrochemical immunoassay was used for comparison purposes. They were classified in three different PSA concentration ranges (0, <4 and ≥4 ng/mL). Cells membrane-engineered with 0.25 μg/mL anti-PSA antibody demonstrated a statistically lower response against the upper (≥4 ng/mL) PSA concentration range. In addition, the cell-based biosensor performed better than the immunosensor in terms of sensitivity and resolution against positive samples containing <4 ng/mL PSA. In spite of its preliminary, proof-of-concept stage of development, the cell-based biosensor could be used as aninitiative for the development of a fast, low-cost, and high-throughput POC screening system for PSA. |
| first_indexed | 2024-04-11T13:18:24Z |
| format | Article |
| id | doaj.art-b2f3049addcf4abcaa481444edea4bcf |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T13:18:24Z |
| publishDate | 2018-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-b2f3049addcf4abcaa481444edea4bcf2022-12-22T04:22:20ZengMDPI AGSensors1424-82202018-11-011811383410.3390/s18113834s18113834An Ultra-Rapid Biosensory Point-of-Care (POC) Assay for Prostate-Specific Antigen (PSA) Detection in Human SerumSophie Mavrikou0Georgia Moschopoulou1Athanasios Zafeirakis2Konstantina Kalogeropoulou3Georgios Giannakos4Athanasios Skevis5Spyridon Kintzios6Laboratory of Cell Technology, Faculty of Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855Athens, GreeceLaboratory of Cell Technology, Faculty of Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855Athens, GreeceArmy Share Fund Hospital of Athens, Monis Petraki 10, 11521 Athens, GreeceArmy Share Fund Hospital of Athens, Monis Petraki 10, 11521 Athens, GreeceArmy Share Fund Hospital of Athens, Monis Petraki 10, 11521 Athens, GreeceLaboratory of Cell Technology, Faculty of Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855Athens, GreeceLaboratory of Cell Technology, Faculty of Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855Athens, GreeceProstate-specific antigen (PSA) is the established routine screening tool for the detection of early-stage prostate cancer. Given the laboratory-centric nature of the process, the development of a portable, ultra rapid high-throughput system for PSA screening is highly desirable. In this study, an advancedpoint-of-care system for PSA detection in human serum was developed based on a cellular biosensor where the cell membrane was modified by electroinserting a specific antibody against PSA. Thirty nine human serum samples were used for validation of this biosensory system for PSA detection. Samples were analyzed in parallel with a standard immunoradiometric assay (IRMA) and an established electrochemical immunoassay was used for comparison purposes. They were classified in three different PSA concentration ranges (0, <4 and ≥4 ng/mL). Cells membrane-engineered with 0.25 μg/mL anti-PSA antibody demonstrated a statistically lower response against the upper (≥4 ng/mL) PSA concentration range. In addition, the cell-based biosensor performed better than the immunosensor in terms of sensitivity and resolution against positive samples containing <4 ng/mL PSA. In spite of its preliminary, proof-of-concept stage of development, the cell-based biosensor could be used as aninitiative for the development of a fast, low-cost, and high-throughput POC screening system for PSA.https://www.mdpi.com/1424-8220/18/11/3834bioelectric recognition assaycellular biosensorhigh-throughputimmunoassaymolecular identification through membrane engineeringpoint-of-careprostate cancerprostate-specific antigen |
| spellingShingle | Sophie Mavrikou Georgia Moschopoulou Athanasios Zafeirakis Konstantina Kalogeropoulou Georgios Giannakos Athanasios Skevis Spyridon Kintzios An Ultra-Rapid Biosensory Point-of-Care (POC) Assay for Prostate-Specific Antigen (PSA) Detection in Human Serum Sensors bioelectric recognition assay cellular biosensor high-throughput immunoassay molecular identification through membrane engineering point-of-care prostate cancer prostate-specific antigen |
| title | An Ultra-Rapid Biosensory Point-of-Care (POC) Assay for Prostate-Specific Antigen (PSA) Detection in Human Serum |
| title_full | An Ultra-Rapid Biosensory Point-of-Care (POC) Assay for Prostate-Specific Antigen (PSA) Detection in Human Serum |
| title_fullStr | An Ultra-Rapid Biosensory Point-of-Care (POC) Assay for Prostate-Specific Antigen (PSA) Detection in Human Serum |
| title_full_unstemmed | An Ultra-Rapid Biosensory Point-of-Care (POC) Assay for Prostate-Specific Antigen (PSA) Detection in Human Serum |
| title_short | An Ultra-Rapid Biosensory Point-of-Care (POC) Assay for Prostate-Specific Antigen (PSA) Detection in Human Serum |
| title_sort | ultra rapid biosensory point of care poc assay for prostate specific antigen psa detection in human serum |
| topic | bioelectric recognition assay cellular biosensor high-throughput immunoassay molecular identification through membrane engineering point-of-care prostate cancer prostate-specific antigen |
| url | https://www.mdpi.com/1424-8220/18/11/3834 |
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