Exploring the <i>Artemisia</i> Genus: An Insight into the Phytochemical and Multi-Biological Potential of <i>A. campestris</i> subsp. <i>lednicensis</i> (Spreng.) Greuter & Raab-Straube
The <i>Artemisia</i> L. genus includes over five hundred species with great economic and medicinal properties. Our study aimed to provide a comprehensive metabolite and bioactivity profile of <i>Artemisia campestris</i> subsp. <i>lednicensis</i> (Spreng.) Greuter...
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MDPI AG
2022-10-01
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| Online Access: | https://www.mdpi.com/2223-7747/11/21/2874 |
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| author | Adriana Trifan Monika E. Czerwińska Constantin Mardari Gokhan Zengin Kouadio Ibrahime Sinan Izabela Korona-Glowniak Krystyna Skalicka-Woźniak Simon Vlad Luca |
| author_facet | Adriana Trifan Monika E. Czerwińska Constantin Mardari Gokhan Zengin Kouadio Ibrahime Sinan Izabela Korona-Glowniak Krystyna Skalicka-Woźniak Simon Vlad Luca |
| author_sort | Adriana Trifan |
| collection | DOAJ |
| description | The <i>Artemisia</i> L. genus includes over five hundred species with great economic and medicinal properties. Our study aimed to provide a comprehensive metabolite and bioactivity profile of <i>Artemisia campestris</i> subsp. <i>lednicensis</i> (Spreng.) Greuter & Raab-Straube collected from north-eastern Romania. Liquid chromatography with tandem high-resolution mass spectrometry (LC-HRMS/MS) analysis of different polarity extracts obtained from the aerial parts led to the identification of twelve flavonoids, three phenolic acids, two sesquiterpene lactones, two fatty acids, one coumarin, and one lignan. The antioxidant and enzyme inhibitory properties were shown in the DPPH (0.71–213.68 mg TE/g) and ABTS (20.57–356.35 mg TE/g) radical scavenging, CUPRAC (38.56–311.21 mg TE/g), FRAP (121.68–202.34 mg TE/g), chelating (12.88–22.25 mg EDTAE/g), phosphomolybdenum (0.92–2.11 mmol TE/g), anti-acetylcholinesterase (0.15–3.64 mg GALAE/g), anti-butyrylcholinesterase (0–3.18 mg GALAE/g), anti-amylase (0.05–0.38 mmol ACAE/g), anti-glucosidase (0.43–2.21 mmol ACAE/g), and anti-tyrosinase (18.62–48.60 mg KAE/g) assays. At 100 μg/mL, <i>Artemisia</i> extracts downregulated the secretion of tumor necrosis factor (TNF)-α in a lipopolysaccharide (LPS)-stimulated human neutrophil model (29.05–53.08% of LPS+ control). Finally, the <i>Artemisia</i> samples showed moderate to weak activity (minimum inhibitory concentration (MIC) > 625 mg/L) against the seventeen tested microbial strains (bacteria, yeasts, and dermatophytes). Overall, our study shows that <i>A. campestris</i> subsp. <i>lednicensis</i> is a promising source of bioactives with putative use as food, pharmaceutical and cosmetic ingredients. |
| first_indexed | 2024-03-09T18:44:21Z |
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| language | English |
| last_indexed | 2024-03-09T18:44:21Z |
| publishDate | 2022-10-01 |
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| spelling | doaj.art-b2f38f79f20d4e38ae5186a814f123d52023-11-24T06:24:27ZengMDPI AGPlants2223-77472022-10-011121287410.3390/plants11212874Exploring the <i>Artemisia</i> Genus: An Insight into the Phytochemical and Multi-Biological Potential of <i>A. campestris</i> subsp. <i>lednicensis</i> (Spreng.) Greuter & Raab-StraubeAdriana Trifan0Monika E. Czerwińska1Constantin Mardari2Gokhan Zengin3Kouadio Ibrahime Sinan4Izabela Korona-Glowniak5Krystyna Skalicka-Woźniak6Simon Vlad Luca7Department of Pharmacognosy, Faculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy Iasi, 700115 Iasi, RomaniaDepartment of Biochemistry and Pharmacogenomics, Faculty of Pharmacy, Medical University of Warsaw, 02-097 Warsaw, PolandBotanic Garden “Anastasie Fatu”, 700471 Iasi, RomaniaPhysiology and Biochemistry Research Laboratory, Department of Biology, Science Faculty, Selcuk University, Konya 42130, TurkeyPhysiology and Biochemistry Research Laboratory, Department of Biology, Science Faculty, Selcuk University, Konya 42130, TurkeyDepartment of Pharmaceutical Microbiology, Faculty of Pharmacy, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Natural Products Chemistry, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Pharmacognosy, Faculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy Iasi, 700115 Iasi, RomaniaThe <i>Artemisia</i> L. genus includes over five hundred species with great economic and medicinal properties. Our study aimed to provide a comprehensive metabolite and bioactivity profile of <i>Artemisia campestris</i> subsp. <i>lednicensis</i> (Spreng.) Greuter & Raab-Straube collected from north-eastern Romania. Liquid chromatography with tandem high-resolution mass spectrometry (LC-HRMS/MS) analysis of different polarity extracts obtained from the aerial parts led to the identification of twelve flavonoids, three phenolic acids, two sesquiterpene lactones, two fatty acids, one coumarin, and one lignan. The antioxidant and enzyme inhibitory properties were shown in the DPPH (0.71–213.68 mg TE/g) and ABTS (20.57–356.35 mg TE/g) radical scavenging, CUPRAC (38.56–311.21 mg TE/g), FRAP (121.68–202.34 mg TE/g), chelating (12.88–22.25 mg EDTAE/g), phosphomolybdenum (0.92–2.11 mmol TE/g), anti-acetylcholinesterase (0.15–3.64 mg GALAE/g), anti-butyrylcholinesterase (0–3.18 mg GALAE/g), anti-amylase (0.05–0.38 mmol ACAE/g), anti-glucosidase (0.43–2.21 mmol ACAE/g), and anti-tyrosinase (18.62–48.60 mg KAE/g) assays. At 100 μg/mL, <i>Artemisia</i> extracts downregulated the secretion of tumor necrosis factor (TNF)-α in a lipopolysaccharide (LPS)-stimulated human neutrophil model (29.05–53.08% of LPS+ control). Finally, the <i>Artemisia</i> samples showed moderate to weak activity (minimum inhibitory concentration (MIC) > 625 mg/L) against the seventeen tested microbial strains (bacteria, yeasts, and dermatophytes). Overall, our study shows that <i>A. campestris</i> subsp. <i>lednicensis</i> is a promising source of bioactives with putative use as food, pharmaceutical and cosmetic ingredients.https://www.mdpi.com/2223-7747/11/21/2874wormwoodLC-HRMS/MSflavonoidsantioxidantanti-enzymaticpro-inflammatory cytokines |
| spellingShingle | Adriana Trifan Monika E. Czerwińska Constantin Mardari Gokhan Zengin Kouadio Ibrahime Sinan Izabela Korona-Glowniak Krystyna Skalicka-Woźniak Simon Vlad Luca Exploring the <i>Artemisia</i> Genus: An Insight into the Phytochemical and Multi-Biological Potential of <i>A. campestris</i> subsp. <i>lednicensis</i> (Spreng.) Greuter & Raab-Straube Plants wormwood LC-HRMS/MS flavonoids antioxidant anti-enzymatic pro-inflammatory cytokines |
| title | Exploring the <i>Artemisia</i> Genus: An Insight into the Phytochemical and Multi-Biological Potential of <i>A. campestris</i> subsp. <i>lednicensis</i> (Spreng.) Greuter & Raab-Straube |
| title_full | Exploring the <i>Artemisia</i> Genus: An Insight into the Phytochemical and Multi-Biological Potential of <i>A. campestris</i> subsp. <i>lednicensis</i> (Spreng.) Greuter & Raab-Straube |
| title_fullStr | Exploring the <i>Artemisia</i> Genus: An Insight into the Phytochemical and Multi-Biological Potential of <i>A. campestris</i> subsp. <i>lednicensis</i> (Spreng.) Greuter & Raab-Straube |
| title_full_unstemmed | Exploring the <i>Artemisia</i> Genus: An Insight into the Phytochemical and Multi-Biological Potential of <i>A. campestris</i> subsp. <i>lednicensis</i> (Spreng.) Greuter & Raab-Straube |
| title_short | Exploring the <i>Artemisia</i> Genus: An Insight into the Phytochemical and Multi-Biological Potential of <i>A. campestris</i> subsp. <i>lednicensis</i> (Spreng.) Greuter & Raab-Straube |
| title_sort | exploring the i artemisia i genus an insight into the phytochemical and multi biological potential of i a campestris i subsp i lednicensis i spreng greuter raab straube |
| topic | wormwood LC-HRMS/MS flavonoids antioxidant anti-enzymatic pro-inflammatory cytokines |
| url | https://www.mdpi.com/2223-7747/11/21/2874 |
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