Detection of protease and lipase activity in Pseudomonas fluorescens isolated from milk and surfaces as milk deteriorating indicators
Raw milk exposure to many sources of contamination and cold storage is a big problem and may impact milk quality, especially the psychotropic Pseudomonas fluorescens a predominant bacterium causing milk spoilage. Some P. fluorescens strains can produce protease and lipase exoenzymes accelerating the...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | Arabic |
| Published: |
University of Mosul, College of Veterinary Medicine
2024-10-01
|
| Series: | Iraqi Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.vetmedmosul.com/article_184725_9ec4e2cc16ac75f2285a7c14b66d7a9a.pdf |
| _version_ | 1827094438057345024 |
|---|---|
| author | Ahmed H. Ahmed Muntaha G. Hasan |
| author_facet | Ahmed H. Ahmed Muntaha G. Hasan |
| author_sort | Ahmed H. Ahmed |
| collection | DOAJ |
| description | Raw milk exposure to many sources of contamination and cold storage is a big problem and may impact milk quality, especially the psychotropic Pseudomonas fluorescens a predominant bacterium causing milk spoilage. Some P. fluorescens strains can produce protease and lipase exoenzymes accelerating the milk spoilage process and reducing the shelf-life of milk and dairy products. Thirty-nine isolates of P. fluorescens isolated from raw cow milk, teat surfaces and milk tanks were examined to detect their abilities to produce both protease and lipase enzymes. These enzymes may increase the virulence of this bacterium based on the detection of both aprX (1434bp) and lip (1422bp) genes using polymerase chain reaction. Results of this study revealed the detection of the aprX gene in (36.8%) of P. fluorescens isolates from raw milk and (81.8%) in bacterium isolated from teat surfaces However, their ability to produce lipase enzymes was reduced by detecting lip genes in (26.3%, 9.1%, and 33.3%) of P. fluorescens isolates from milk, teat surfaces and milk tanks respectively.Theses results confirm the potential of P. fluorescens to induce spoilage in milk and dairy products processed from milk through protease secretion which causes casein hydrolysis and indicates prognosis about the hygienic procedures during milking and processing affecting the milk shelf-life period. |
| first_indexed | 2025-03-20T06:43:59Z |
| format | Article |
| id | doaj.art-b2fc19ff68a842b0a25bce7ed085bcaf |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2025-03-20T06:43:59Z |
| publishDate | 2024-10-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-b2fc19ff68a842b0a25bce7ed085bcaf2024-10-02T02:50:38ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552024-10-0138478779110.33899/ijvs.2024.148932.3621184725Detection of protease and lipase activity in Pseudomonas fluorescens isolated from milk and surfaces as milk deteriorating indicatorsAhmed H. Ahmed0Muntaha G. Hasan1Nineveh Health Directorate, Mosul, IraqDepartment of Veterinary Public Health, College of Veterinary Medicine, University of Mosul, Mosul, IraqRaw milk exposure to many sources of contamination and cold storage is a big problem and may impact milk quality, especially the psychotropic Pseudomonas fluorescens a predominant bacterium causing milk spoilage. Some P. fluorescens strains can produce protease and lipase exoenzymes accelerating the milk spoilage process and reducing the shelf-life of milk and dairy products. Thirty-nine isolates of P. fluorescens isolated from raw cow milk, teat surfaces and milk tanks were examined to detect their abilities to produce both protease and lipase enzymes. These enzymes may increase the virulence of this bacterium based on the detection of both aprX (1434bp) and lip (1422bp) genes using polymerase chain reaction. Results of this study revealed the detection of the aprX gene in (36.8%) of P. fluorescens isolates from raw milk and (81.8%) in bacterium isolated from teat surfaces However, their ability to produce lipase enzymes was reduced by detecting lip genes in (26.3%, 9.1%, and 33.3%) of P. fluorescens isolates from milk, teat surfaces and milk tanks respectively.Theses results confirm the potential of P. fluorescens to induce spoilage in milk and dairy products processed from milk through protease secretion which causes casein hydrolysis and indicates prognosis about the hygienic procedures during milking and processing affecting the milk shelf-life period.https://www.vetmedmosul.com/article_184725_9ec4e2cc16ac75f2285a7c14b66d7a9a.pdfpseudomonas fluorescensexoenzymesmilk |
| spellingShingle | Ahmed H. Ahmed Muntaha G. Hasan Detection of protease and lipase activity in Pseudomonas fluorescens isolated from milk and surfaces as milk deteriorating indicators Iraqi Journal of Veterinary Sciences pseudomonas fluorescens exoenzymes milk |
| title | Detection of protease and lipase activity in Pseudomonas fluorescens isolated from milk and surfaces as milk deteriorating indicators |
| title_full | Detection of protease and lipase activity in Pseudomonas fluorescens isolated from milk and surfaces as milk deteriorating indicators |
| title_fullStr | Detection of protease and lipase activity in Pseudomonas fluorescens isolated from milk and surfaces as milk deteriorating indicators |
| title_full_unstemmed | Detection of protease and lipase activity in Pseudomonas fluorescens isolated from milk and surfaces as milk deteriorating indicators |
| title_short | Detection of protease and lipase activity in Pseudomonas fluorescens isolated from milk and surfaces as milk deteriorating indicators |
| title_sort | detection of protease and lipase activity in pseudomonas fluorescens isolated from milk and surfaces as milk deteriorating indicators |
| topic | pseudomonas fluorescens exoenzymes milk |
| url | https://www.vetmedmosul.com/article_184725_9ec4e2cc16ac75f2285a7c14b66d7a9a.pdf |
| work_keys_str_mv | AT ahmedhahmed detectionofproteaseandlipaseactivityinpseudomonasfluorescensisolatedfrommilkandsurfacesasmilkdeterioratingindicators AT muntahaghasan detectionofproteaseandlipaseactivityinpseudomonasfluorescensisolatedfrommilkandsurfacesasmilkdeterioratingindicators |