Intranasal powder live attenuated influenza vaccine is thermostable, immunogenic, and protective against homologous challenge in ferrets
Abstract Influenza viruses cause annual seasonal epidemics and sporadic pandemics; vaccination is the most effective countermeasure. Intranasal live attenuated influenza vaccines (LAIVs) are needle-free, mimic the natural route of infection, and elicit robust immunity. However, some LAIVs require re...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-04-01
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| Series: | npj Vaccines |
| Online Access: | https://doi.org/10.1038/s41541-021-00320-9 |
| _version_ | 1827616261404622848 |
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| author | Jasmina M. Luczo Tatiana Bousse Scott K. Johnson Cheryl A. Jones Nicholas Pearce Carlie A. Neiswanger Min-Xuan Wang Erin A. Miller Nikolai Petrovsky David E. Wentworth Victor Bronshtein Mark Papania Stephen M. Tompkins |
| author_facet | Jasmina M. Luczo Tatiana Bousse Scott K. Johnson Cheryl A. Jones Nicholas Pearce Carlie A. Neiswanger Min-Xuan Wang Erin A. Miller Nikolai Petrovsky David E. Wentworth Victor Bronshtein Mark Papania Stephen M. Tompkins |
| author_sort | Jasmina M. Luczo |
| collection | DOAJ |
| description | Abstract Influenza viruses cause annual seasonal epidemics and sporadic pandemics; vaccination is the most effective countermeasure. Intranasal live attenuated influenza vaccines (LAIVs) are needle-free, mimic the natural route of infection, and elicit robust immunity. However, some LAIVs require reconstitution and cold-chain requirements restrict storage and distribution of all influenza vaccines. We generated a dry-powder, thermostable LAIV (T-LAIV) using Preservation by Vaporization technology and assessed the stability, immunogenicity, and efficacy of T-LAIV alone or combined with delta inulin adjuvant (Advax™) in ferrets. Stability assays demonstrated minimal loss of T-LAIV titer when stored at 25 °C for 1 year. Vaccination of ferrets with T-LAIV alone or with delta inulin adjuvant elicited mucosal antibody and robust serum HI responses in ferrets, and was protective against homologous challenge. These results suggest that the Preservation by Vaporization-generated dry-powder vaccines could be distributed without refrigeration and administered without reconstitution or injection. Given these significant advantages for vaccine distribution and delivery, further research is warranted. |
| first_indexed | 2024-03-09T09:24:20Z |
| format | Article |
| id | doaj.art-b30473452fc54337aa97734a70ab5f16 |
| institution | Directory Open Access Journal |
| issn | 2059-0105 |
| language | English |
| last_indexed | 2024-03-09T09:24:20Z |
| publishDate | 2021-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Vaccines |
| spelling | doaj.art-b30473452fc54337aa97734a70ab5f162023-12-02T06:46:14ZengNature Portfolionpj Vaccines2059-01052021-04-01611810.1038/s41541-021-00320-9Intranasal powder live attenuated influenza vaccine is thermostable, immunogenic, and protective against homologous challenge in ferretsJasmina M. Luczo0Tatiana Bousse1Scott K. Johnson2Cheryl A. Jones3Nicholas Pearce4Carlie A. Neiswanger5Min-Xuan Wang6Erin A. Miller7Nikolai Petrovsky8David E. Wentworth9Victor Bronshtein10Mark Papania11Stephen M. Tompkins12Center for Vaccines and Immunology, University of GeorgiaInfluenza Division, Centers for Disease Control and PreventionCenter for Vaccines and Immunology, University of GeorgiaCenter for Vaccines and Immunology, University of GeorgiaInfluenza Division, Centers for Disease Control and PreventionCenter for Vaccines and Immunology, University of GeorgiaUniversal Stabilization Technologies, Inc.Universal Stabilization Technologies, Inc.College of Medicine and Public Health, Flinders UniversityInfluenza Division, Centers for Disease Control and PreventionUniversal Stabilization Technologies, Inc.Global Immunization Division, Centers for Disease Control and PreventionCenter for Vaccines and Immunology, University of GeorgiaAbstract Influenza viruses cause annual seasonal epidemics and sporadic pandemics; vaccination is the most effective countermeasure. Intranasal live attenuated influenza vaccines (LAIVs) are needle-free, mimic the natural route of infection, and elicit robust immunity. However, some LAIVs require reconstitution and cold-chain requirements restrict storage and distribution of all influenza vaccines. We generated a dry-powder, thermostable LAIV (T-LAIV) using Preservation by Vaporization technology and assessed the stability, immunogenicity, and efficacy of T-LAIV alone or combined with delta inulin adjuvant (Advax™) in ferrets. Stability assays demonstrated minimal loss of T-LAIV titer when stored at 25 °C for 1 year. Vaccination of ferrets with T-LAIV alone or with delta inulin adjuvant elicited mucosal antibody and robust serum HI responses in ferrets, and was protective against homologous challenge. These results suggest that the Preservation by Vaporization-generated dry-powder vaccines could be distributed without refrigeration and administered without reconstitution or injection. Given these significant advantages for vaccine distribution and delivery, further research is warranted.https://doi.org/10.1038/s41541-021-00320-9 |
| spellingShingle | Jasmina M. Luczo Tatiana Bousse Scott K. Johnson Cheryl A. Jones Nicholas Pearce Carlie A. Neiswanger Min-Xuan Wang Erin A. Miller Nikolai Petrovsky David E. Wentworth Victor Bronshtein Mark Papania Stephen M. Tompkins Intranasal powder live attenuated influenza vaccine is thermostable, immunogenic, and protective against homologous challenge in ferrets npj Vaccines |
| title | Intranasal powder live attenuated influenza vaccine is thermostable, immunogenic, and protective against homologous challenge in ferrets |
| title_full | Intranasal powder live attenuated influenza vaccine is thermostable, immunogenic, and protective against homologous challenge in ferrets |
| title_fullStr | Intranasal powder live attenuated influenza vaccine is thermostable, immunogenic, and protective against homologous challenge in ferrets |
| title_full_unstemmed | Intranasal powder live attenuated influenza vaccine is thermostable, immunogenic, and protective against homologous challenge in ferrets |
| title_short | Intranasal powder live attenuated influenza vaccine is thermostable, immunogenic, and protective against homologous challenge in ferrets |
| title_sort | intranasal powder live attenuated influenza vaccine is thermostable immunogenic and protective against homologous challenge in ferrets |
| url | https://doi.org/10.1038/s41541-021-00320-9 |
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