Effect of drought on root exudates from Quercus petraea and enzymatic activity of soil
Abstract Root exudation is a key process that determines rhizosphere functions and plant-soil relationships. The present study was conducted with the objectives to (1) determine the root morphology of sessile oak seedlings in relation to drought, (2) assess root exudation and its response to drought...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-11754-z |
| _version_ | 1818210289149018112 |
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| author | Karolina Staszel Jarosław Lasota Ewa Błońska |
| author_facet | Karolina Staszel Jarosław Lasota Ewa Błońska |
| author_sort | Karolina Staszel |
| collection | DOAJ |
| description | Abstract Root exudation is a key process that determines rhizosphere functions and plant-soil relationships. The present study was conducted with the objectives to (1) determine the root morphology of sessile oak seedlings in relation to drought, (2) assess root exudation and its response to drought, and (3) detect possible changes in the activity of soil enzymes in response to drought enhancement. In the experiment, sessile oak seedlings (Quercus petraea Matt.) were used, and two variants of substrate moisture (25% humidity—dry variant and 55% humidity—fresh variant) on which oaks grew were considered. Exudates were collected using a culture-based cuvette system. Results confirmed the importance of drought in shaping the morphology of roots and root carbon exudation of sessile oak. The oak roots in the dry variant responded with a higher increment in length. In the case of roots growing in higher humidity, a higher specific root area and specific root length were determined. Experimental evidence has demonstrated decreased root exudation under dry conditions, which can lead to a change in enzyme activity. In the study, enzyme activity decreased by 90% for β-D-cellobiosidase (CB), 50% for β-glucosidase (BG) and N-acetyl-β-D-glucosaminidase (NAG), 20% for β-xylosidase (XYL) decreased by, and the activity of arylsulphatase (SP) and phosphatase (PH) decreased by 10%. |
| first_indexed | 2024-12-12T05:14:14Z |
| format | Article |
| id | doaj.art-b3047730ebb14281bc9291dad6f7442d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T05:14:14Z |
| publishDate | 2022-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b3047730ebb14281bc9291dad6f7442d2022-12-22T00:36:49ZengNature PortfolioScientific Reports2045-23222022-05-011211810.1038/s41598-022-11754-zEffect of drought on root exudates from Quercus petraea and enzymatic activity of soilKarolina Staszel0Jarosław Lasota1Ewa Błońska2Department of Ecology and Silviculture, Faculty of Forestry, University of Agriculture in KrakowDepartment of Ecology and Silviculture, Faculty of Forestry, University of Agriculture in KrakowDepartment of Ecology and Silviculture, Faculty of Forestry, University of Agriculture in KrakowAbstract Root exudation is a key process that determines rhizosphere functions and plant-soil relationships. The present study was conducted with the objectives to (1) determine the root morphology of sessile oak seedlings in relation to drought, (2) assess root exudation and its response to drought, and (3) detect possible changes in the activity of soil enzymes in response to drought enhancement. In the experiment, sessile oak seedlings (Quercus petraea Matt.) were used, and two variants of substrate moisture (25% humidity—dry variant and 55% humidity—fresh variant) on which oaks grew were considered. Exudates were collected using a culture-based cuvette system. Results confirmed the importance of drought in shaping the morphology of roots and root carbon exudation of sessile oak. The oak roots in the dry variant responded with a higher increment in length. In the case of roots growing in higher humidity, a higher specific root area and specific root length were determined. Experimental evidence has demonstrated decreased root exudation under dry conditions, which can lead to a change in enzyme activity. In the study, enzyme activity decreased by 90% for β-D-cellobiosidase (CB), 50% for β-glucosidase (BG) and N-acetyl-β-D-glucosaminidase (NAG), 20% for β-xylosidase (XYL) decreased by, and the activity of arylsulphatase (SP) and phosphatase (PH) decreased by 10%.https://doi.org/10.1038/s41598-022-11754-z |
| spellingShingle | Karolina Staszel Jarosław Lasota Ewa Błońska Effect of drought on root exudates from Quercus petraea and enzymatic activity of soil Scientific Reports |
| title | Effect of drought on root exudates from Quercus petraea and enzymatic activity of soil |
| title_full | Effect of drought on root exudates from Quercus petraea and enzymatic activity of soil |
| title_fullStr | Effect of drought on root exudates from Quercus petraea and enzymatic activity of soil |
| title_full_unstemmed | Effect of drought on root exudates from Quercus petraea and enzymatic activity of soil |
| title_short | Effect of drought on root exudates from Quercus petraea and enzymatic activity of soil |
| title_sort | effect of drought on root exudates from quercus petraea and enzymatic activity of soil |
| url | https://doi.org/10.1038/s41598-022-11754-z |
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