AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development
The expression of AtchitIV gene was analysed in Arabidopsis plants submitted to abiotic stresses. Transcript accumulation was detected in leaves in response to UV light exposure, exogenous salicylic acid administration and wounding. Transgenic Arabidopsis plants carrying AtchitIV promoter::gus fusio...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2004-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100020 |
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| author | Liliane B. de A. Gerhardt Cláudia Magioli Ana B.U.C.M. Perez Rogério Margis Gilberto Sachetto-Martins Márcia Margis-Pinheiro |
| author_facet | Liliane B. de A. Gerhardt Cláudia Magioli Ana B.U.C.M. Perez Rogério Margis Gilberto Sachetto-Martins Márcia Margis-Pinheiro |
| author_sort | Liliane B. de A. Gerhardt |
| collection | DOAJ |
| description | The expression of AtchitIV gene was analysed in Arabidopsis plants submitted to abiotic stresses. Transcript accumulation was detected in leaves in response to UV light exposure, exogenous salicylic acid administration and wounding. Transgenic Arabidopsis plants carrying AtchitIV promoter::gus fusion also showed differential expression of the reporter gene in response to these treatments. The AtchitIV expression was also analysed during Arabidopsis embryo development. GUS assay demonstrated AtchitIV promoter activation in zygotic embryos from torpedo stage up to full maturation. Promoter deletion analysis indicated that all the 5' cis-acting elements responsible for the specific tissue expression are located in a region of 1083 bp, adjacent to the start of transcription. A negative regulatory region located between portions -1083 and -600 was also observed. |
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| format | Article |
| id | doaj.art-b30497df6fab42e29ef8cf9cbe51696c |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-11T04:11:24Z |
| publishDate | 2004-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-b30497df6fab42e29ef8cf9cbe51696c2022-12-22T01:21:22ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852004-01-0127111812310.1590/S1415-47572004000100020AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo developmentLiliane B. de A. GerhardtCláudia MagioliAna B.U.C.M. PerezRogério MargisGilberto Sachetto-MartinsMárcia Margis-PinheiroThe expression of AtchitIV gene was analysed in Arabidopsis plants submitted to abiotic stresses. Transcript accumulation was detected in leaves in response to UV light exposure, exogenous salicylic acid administration and wounding. Transgenic Arabidopsis plants carrying AtchitIV promoter::gus fusion also showed differential expression of the reporter gene in response to these treatments. The AtchitIV expression was also analysed during Arabidopsis embryo development. GUS assay demonstrated AtchitIV promoter activation in zygotic embryos from torpedo stage up to full maturation. Promoter deletion analysis indicated that all the 5' cis-acting elements responsible for the specific tissue expression are located in a region of 1083 bp, adjacent to the start of transcription. A negative regulatory region located between portions -1083 and -600 was also observed.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100020abiotic stressChia4 Arabidopsis thaliana endochitinaseembryogenesisgene expressionpromoter |
| spellingShingle | Liliane B. de A. Gerhardt Cláudia Magioli Ana B.U.C.M. Perez Rogério Margis Gilberto Sachetto-Martins Márcia Margis-Pinheiro AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development Genetics and Molecular Biology abiotic stress Chia4 Arabidopsis thaliana endochitinase embryogenesis gene expression promoter |
| title | AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development |
| title_full | AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development |
| title_fullStr | AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development |
| title_full_unstemmed | AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development |
| title_short | AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development |
| title_sort | atchitiv gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development |
| topic | abiotic stress Chia4 Arabidopsis thaliana endochitinase embryogenesis gene expression promoter |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100020 |
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