Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migration
It is currently challenging to adequately model the growth and migration of glioblastoma using two‐dimensional (2D) in vitro culture systems as they quickly lose the original, patient‐specific identity and heterogeneity. However, with the advent of three‐dimensional (3D) cell cultures and human‐indu...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-04-01
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| Series: | Molecular Oncology |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/1878-0261.13389 |
| _version_ | 1827976474957709312 |
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| author | Veronika Fedorova Veronika Pospisilova Tereza Vanova Katerina Amruz Cerna Pavel Abaffy Jiri Sedmik Jan Raska Simona Vochyanova Zuzana Matusova Jana Houserova Lukas Valihrach Zdenek Hodny Dasa Bohaciakova |
| author_facet | Veronika Fedorova Veronika Pospisilova Tereza Vanova Katerina Amruz Cerna Pavel Abaffy Jiri Sedmik Jan Raska Simona Vochyanova Zuzana Matusova Jana Houserova Lukas Valihrach Zdenek Hodny Dasa Bohaciakova |
| author_sort | Veronika Fedorova |
| collection | DOAJ |
| description | It is currently challenging to adequately model the growth and migration of glioblastoma using two‐dimensional (2D) in vitro culture systems as they quickly lose the original, patient‐specific identity and heterogeneity. However, with the advent of three‐dimensional (3D) cell cultures and human‐induced pluripotent stem cell (iPSC)‐derived cerebral organoids (COs), studies demonstrate that the glioblastoma‐CO (GLICO) coculture model helps to preserve the phenotype of the patient‐specific tissue. Here, we aimed to set up such a model using mature COs and develop a pipeline for subsequent analysis of cocultured glioblastoma. Our data demonstrate that the growth and migration of the glioblastoma cell line within the mature COs are significantly increased in the presence of extracellular matrix proteins, shortening the time needed for glioblastoma to initiate migration. We also describe in detail the method for the visualization and quantification of these migrating cells within the GLICO model. Lastly, we show that this coculture model (and the human brain‐like microenvironment) can significantly transform the gene expression profile of the established U87 glioblastoma cell line into proneural and classical glioblastoma cell types. |
| first_indexed | 2024-04-09T20:36:01Z |
| format | Article |
| id | doaj.art-b304c5e41f08440b9da8a1c990ebbc79 |
| institution | Directory Open Access Journal |
| issn | 1574-7891 1878-0261 |
| language | English |
| last_indexed | 2024-04-09T20:36:01Z |
| publishDate | 2023-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Molecular Oncology |
| spelling | doaj.art-b304c5e41f08440b9da8a1c990ebbc792023-03-30T08:40:48ZengWileyMolecular Oncology1574-78911878-02612023-04-0117464766310.1002/1878-0261.13389Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migrationVeronika Fedorova0Veronika Pospisilova1Tereza Vanova2Katerina Amruz Cerna3Pavel Abaffy4Jiri Sedmik5Jan Raska6Simona Vochyanova7Zuzana Matusova8Jana Houserova9Lukas Valihrach10Zdenek Hodny11Dasa Bohaciakova12Department of Histology and Embryology, Faculty of Medicine Masaryk University Brno Czech RepublicDepartment of Histology and Embryology, Faculty of Medicine Masaryk University Brno Czech RepublicDepartment of Histology and Embryology, Faculty of Medicine Masaryk University Brno Czech RepublicDepartment of Histology and Embryology, Faculty of Medicine Masaryk University Brno Czech RepublicLaboratory of Gene Expression Institute of Biotechnology CAS, BIOCEV Vestec Czech RepublicDepartment of Histology and Embryology, Faculty of Medicine Masaryk University Brno Czech RepublicDepartment of Histology and Embryology, Faculty of Medicine Masaryk University Brno Czech RepublicDepartment of Histology and Embryology, Faculty of Medicine Masaryk University Brno Czech RepublicLaboratory of Gene Expression Institute of Biotechnology CAS, BIOCEV Vestec Czech RepublicDepartment of Genome Integrity Institute of Molecular Genetics of the Czech Academy of Sciences Prague Czech RepublicLaboratory of Gene Expression Institute of Biotechnology CAS, BIOCEV Vestec Czech RepublicDepartment of Genome Integrity Institute of Molecular Genetics of the Czech Academy of Sciences Prague Czech RepublicDepartment of Histology and Embryology, Faculty of Medicine Masaryk University Brno Czech RepublicIt is currently challenging to adequately model the growth and migration of glioblastoma using two‐dimensional (2D) in vitro culture systems as they quickly lose the original, patient‐specific identity and heterogeneity. However, with the advent of three‐dimensional (3D) cell cultures and human‐induced pluripotent stem cell (iPSC)‐derived cerebral organoids (COs), studies demonstrate that the glioblastoma‐CO (GLICO) coculture model helps to preserve the phenotype of the patient‐specific tissue. Here, we aimed to set up such a model using mature COs and develop a pipeline for subsequent analysis of cocultured glioblastoma. Our data demonstrate that the growth and migration of the glioblastoma cell line within the mature COs are significantly increased in the presence of extracellular matrix proteins, shortening the time needed for glioblastoma to initiate migration. We also describe in detail the method for the visualization and quantification of these migrating cells within the GLICO model. Lastly, we show that this coculture model (and the human brain‐like microenvironment) can significantly transform the gene expression profile of the established U87 glioblastoma cell line into proneural and classical glioblastoma cell types.https://doi.org/10.1002/1878-0261.13389cerebral organoidsGLICOglioblastomainduced pluripotent stem cells |
| spellingShingle | Veronika Fedorova Veronika Pospisilova Tereza Vanova Katerina Amruz Cerna Pavel Abaffy Jiri Sedmik Jan Raska Simona Vochyanova Zuzana Matusova Jana Houserova Lukas Valihrach Zdenek Hodny Dasa Bohaciakova Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migration Molecular Oncology cerebral organoids GLICO glioblastoma induced pluripotent stem cells |
| title | Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migration |
| title_full | Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migration |
| title_fullStr | Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migration |
| title_full_unstemmed | Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migration |
| title_short | Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migration |
| title_sort | glioblastoma and cerebral organoids development and analysis of an in vitro model for glioblastoma migration |
| topic | cerebral organoids GLICO glioblastoma induced pluripotent stem cells |
| url | https://doi.org/10.1002/1878-0261.13389 |
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