Production of fibrinolytic protease from various fungal isolates and species 2.Determination of optimum conditions for enzyme production from Pleurotus ostreatus

The optimum conditions for production of fibrinolytic protease from an edible mushroom Pleurotus ostreatus grown on the solid medium , Sus medium, composed of Sus wastes (produced from extracted medicinal plant Glycyrrhiza glabra) were determined. Addition of 5% of Soya bean seeds meal in Sus...

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Main Author: Baghdad Science Journal
Format: Article
Language:Arabic
Published: College of Science for Women, University of Baghdad 2007-03-01
Series:Baghdad Science Journal
Online Access:http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/762
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author Baghdad Science Journal
author_facet Baghdad Science Journal
author_sort Baghdad Science Journal
collection DOAJ
description The optimum conditions for production of fibrinolytic protease from an edible mushroom Pleurotus ostreatus grown on the solid medium , Sus medium, composed of Sus wastes (produced from extracted medicinal plant Glycyrrhiza glabra) were determined. Addition of 5% of Soya bean seeds meal in Sus medium recorded a maximum fibrinolytic protease activity resulting in 7.7 units / ml. The optimum moisture content of Sus medium supplemented with 5% Soya bean seeds meal was 60% resulting in 7.2 units / ml.Pleurotus ostreatus produced a maximum fibrinolytic protease activity when the spawn rate,pH of medium and incubation temperature were 2,6 and 30°C, respectively. The maximum fibrinolytic protease activity was 7.6 units / ml when incubation period of Pleurotus ostreatus at the end of 3rd week (vegetative or mycelium stage), then lowered to 6.2 and 4.4 units/ml in the end of 4th week (reproduction or fruit bodies stage) and 5th week (after harvesting of fruit bodies), respectively. Although the minimum fibrinolytic protease activity was recorded in the end of 4th and 5th weeks, production of fibrinolytic protease regard to a byproduct after harvesting of fruit bodies.
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spelling doaj.art-b30fe3c3844040dc8f284eb79c0bf1f12022-12-21T23:47:17ZaraCollege of Science for Women, University of BaghdadBaghdad Science Journal2078-86652411-79862007-03-014110.21123/bsj.4.1.14-21Production of fibrinolytic protease from various fungal isolates and species 2.Determination of optimum conditions for enzyme production from Pleurotus ostreatusBaghdad Science JournalThe optimum conditions for production of fibrinolytic protease from an edible mushroom Pleurotus ostreatus grown on the solid medium , Sus medium, composed of Sus wastes (produced from extracted medicinal plant Glycyrrhiza glabra) were determined. Addition of 5% of Soya bean seeds meal in Sus medium recorded a maximum fibrinolytic protease activity resulting in 7.7 units / ml. The optimum moisture content of Sus medium supplemented with 5% Soya bean seeds meal was 60% resulting in 7.2 units / ml.Pleurotus ostreatus produced a maximum fibrinolytic protease activity when the spawn rate,pH of medium and incubation temperature were 2,6 and 30°C, respectively. The maximum fibrinolytic protease activity was 7.6 units / ml when incubation period of Pleurotus ostreatus at the end of 3rd week (vegetative or mycelium stage), then lowered to 6.2 and 4.4 units/ml in the end of 4th week (reproduction or fruit bodies stage) and 5th week (after harvesting of fruit bodies), respectively. Although the minimum fibrinolytic protease activity was recorded in the end of 4th and 5th weeks, production of fibrinolytic protease regard to a byproduct after harvesting of fruit bodies.http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/762
spellingShingle Baghdad Science Journal
Production of fibrinolytic protease from various fungal isolates and species 2.Determination of optimum conditions for enzyme production from Pleurotus ostreatus
Baghdad Science Journal
title Production of fibrinolytic protease from various fungal isolates and species 2.Determination of optimum conditions for enzyme production from Pleurotus ostreatus
title_full Production of fibrinolytic protease from various fungal isolates and species 2.Determination of optimum conditions for enzyme production from Pleurotus ostreatus
title_fullStr Production of fibrinolytic protease from various fungal isolates and species 2.Determination of optimum conditions for enzyme production from Pleurotus ostreatus
title_full_unstemmed Production of fibrinolytic protease from various fungal isolates and species 2.Determination of optimum conditions for enzyme production from Pleurotus ostreatus
title_short Production of fibrinolytic protease from various fungal isolates and species 2.Determination of optimum conditions for enzyme production from Pleurotus ostreatus
title_sort production of fibrinolytic protease from various fungal isolates and species 2 determination of optimum conditions for enzyme production from pleurotus ostreatus
url http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/762
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