Identity of <i>bla</i><sub>CTX-M</sub> Carrying Plasmids in Sequential ESBL-<i>E. coli</i> Isolates from Patients with Recurrent Urinary Tract Infections
Plasmid-mediated multidrug resistance in <i>E. coli</i> is becoming increasingly prevalent. Considering this global threat to human health, it is important to understand how plasmid-mediated resistance spreads. From a cohort of 123 patients with recurrent urinary tract infections (RUTI)...
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MDPI AG
2021-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/6/1138 |
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| author | Nahid Karami Sriram KK Shora Yazdanshenas Yii-Lih Lin Daniel Jaén-Luchoro Elina Ekedahl Sanjana Parameshwaran Anna Lindblom Christina Åhrén Fredrik Westerlund |
| author_facet | Nahid Karami Sriram KK Shora Yazdanshenas Yii-Lih Lin Daniel Jaén-Luchoro Elina Ekedahl Sanjana Parameshwaran Anna Lindblom Christina Åhrén Fredrik Westerlund |
| author_sort | Nahid Karami |
| collection | DOAJ |
| description | Plasmid-mediated multidrug resistance in <i>E. coli</i> is becoming increasingly prevalent. Considering this global threat to human health, it is important to understand how plasmid-mediated resistance spreads. From a cohort of 123 patients with recurrent urinary tract infections (RUTI) due to extended spectrum beta-lactamase (ESBL)-producing <i>Escherichia coli</i> (ESBL <i>E. coli</i>), only five events with a change of ESBL <i>E. coli</i> strain between RUTI episodes were identified. Their <i>bla</i><sub>CTX-M</sub> encoding plasmids were compared within each pair of isolates using optical DNA mapping (ODM) and PCR-based replicon typing. Despite similar <i>bla</i><sub>CTX-M</sub> genes and replicon types, ODM detected only one case with identical plasmids in the sequential ESBL <i>E. coli</i> strains, indicating that plasmid transfer could have occurred. For comparison, plasmids from seven patients with the same ESBL <i>E. coli</i> strain reoccurring in both episodes were analyzed. These plasmids (encoding <i>bla</i><sub>CTX-M-3</sub>, <i>bla</i><sub>CTX-M-14</sub>, and <i>bla</i><sub>CTX-M-15</sub>) were unaltered for up to six months between recurrent infections. Thus, transmission of <i>bla</i><sub>CTX-M</sub> plasmids appears to be a rare event during the course of RUTI. Despite the limited number (<i>n</i> = 23) of plasmids investigated, similar <i>bla</i><sub>CTX-M</sub>-<sub>15</sub> plasmids in unrelated isolates from different patients were detected, suggesting that some successful plasmids could be associated with specific strains, or are more easily transmitted. |
| first_indexed | 2024-03-10T11:03:44Z |
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| id | doaj.art-b311d58f4f31447da44bcc2d697e109e |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T11:03:44Z |
| publishDate | 2021-05-01 |
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| series | Microorganisms |
| spelling | doaj.art-b311d58f4f31447da44bcc2d697e109e2023-11-21T21:18:53ZengMDPI AGMicroorganisms2076-26072021-05-0196113810.3390/microorganisms9061138Identity of <i>bla</i><sub>CTX-M</sub> Carrying Plasmids in Sequential ESBL-<i>E. coli</i> Isolates from Patients with Recurrent Urinary Tract InfectionsNahid Karami0Sriram KK1Shora Yazdanshenas2Yii-Lih Lin3Daniel Jaén-Luchoro4Elina Ekedahl5Sanjana Parameshwaran6Anna Lindblom7Christina Åhrén8Fredrik Westerlund9Institute of Biomedicine, Department of Infectious Diseases and Centre for Antibiotic Resistance Research (CARe), University of Gothenburg, Guldhedsgatan 10 A, 413 46 Gothenburg, SwedenDepartment of Biology and Biological Engineering, Chalmers University of Technology, Kemivagen 10, 412 96 Gothenburg, SwedenInstitute of Biomedicine, Department of Infectious Diseases and Centre for Antibiotic Resistance Research (CARe), University of Gothenburg, Guldhedsgatan 10 A, 413 46 Gothenburg, SwedenDepartment of Biology and Biological Engineering, Chalmers University of Technology, Kemivagen 10, 412 96 Gothenburg, SwedenInstitute of Biomedicine, Department of Infectious Diseases and Centre for Antibiotic Resistance Research (CARe), University of Gothenburg, Guldhedsgatan 10 A, 413 46 Gothenburg, SwedenDepartment of Biology and Biological Engineering, Chalmers University of Technology, Kemivagen 10, 412 96 Gothenburg, SwedenDepartment of Biology and Biological Engineering, Chalmers University of Technology, Kemivagen 10, 412 96 Gothenburg, SwedenInstitute of Biomedicine, Department of Infectious Diseases and Centre for Antibiotic Resistance Research (CARe), University of Gothenburg, Guldhedsgatan 10 A, 413 46 Gothenburg, SwedenInstitute of Biomedicine, Department of Infectious Diseases and Centre for Antibiotic Resistance Research (CARe), University of Gothenburg, Guldhedsgatan 10 A, 413 46 Gothenburg, SwedenDepartment of Biology and Biological Engineering, Chalmers University of Technology, Kemivagen 10, 412 96 Gothenburg, SwedenPlasmid-mediated multidrug resistance in <i>E. coli</i> is becoming increasingly prevalent. Considering this global threat to human health, it is important to understand how plasmid-mediated resistance spreads. From a cohort of 123 patients with recurrent urinary tract infections (RUTI) due to extended spectrum beta-lactamase (ESBL)-producing <i>Escherichia coli</i> (ESBL <i>E. coli</i>), only five events with a change of ESBL <i>E. coli</i> strain between RUTI episodes were identified. Their <i>bla</i><sub>CTX-M</sub> encoding plasmids were compared within each pair of isolates using optical DNA mapping (ODM) and PCR-based replicon typing. Despite similar <i>bla</i><sub>CTX-M</sub> genes and replicon types, ODM detected only one case with identical plasmids in the sequential ESBL <i>E. coli</i> strains, indicating that plasmid transfer could have occurred. For comparison, plasmids from seven patients with the same ESBL <i>E. coli</i> strain reoccurring in both episodes were analyzed. These plasmids (encoding <i>bla</i><sub>CTX-M-3</sub>, <i>bla</i><sub>CTX-M-14</sub>, and <i>bla</i><sub>CTX-M-15</sub>) were unaltered for up to six months between recurrent infections. Thus, transmission of <i>bla</i><sub>CTX-M</sub> plasmids appears to be a rare event during the course of RUTI. Despite the limited number (<i>n</i> = 23) of plasmids investigated, similar <i>bla</i><sub>CTX-M</sub>-<sub>15</sub> plasmids in unrelated isolates from different patients were detected, suggesting that some successful plasmids could be associated with specific strains, or are more easily transmitted.https://www.mdpi.com/2076-2607/9/6/1138extended-spectrum beta-lactamase (ESBL)recurrent urinary tract infectionplasmidshorizontal transfer<i>E. coli</i>optical DNA mapping (ODM) |
| spellingShingle | Nahid Karami Sriram KK Shora Yazdanshenas Yii-Lih Lin Daniel Jaén-Luchoro Elina Ekedahl Sanjana Parameshwaran Anna Lindblom Christina Åhrén Fredrik Westerlund Identity of <i>bla</i><sub>CTX-M</sub> Carrying Plasmids in Sequential ESBL-<i>E. coli</i> Isolates from Patients with Recurrent Urinary Tract Infections Microorganisms extended-spectrum beta-lactamase (ESBL) recurrent urinary tract infection plasmids horizontal transfer <i>E. coli</i> optical DNA mapping (ODM) |
| title | Identity of <i>bla</i><sub>CTX-M</sub> Carrying Plasmids in Sequential ESBL-<i>E. coli</i> Isolates from Patients with Recurrent Urinary Tract Infections |
| title_full | Identity of <i>bla</i><sub>CTX-M</sub> Carrying Plasmids in Sequential ESBL-<i>E. coli</i> Isolates from Patients with Recurrent Urinary Tract Infections |
| title_fullStr | Identity of <i>bla</i><sub>CTX-M</sub> Carrying Plasmids in Sequential ESBL-<i>E. coli</i> Isolates from Patients with Recurrent Urinary Tract Infections |
| title_full_unstemmed | Identity of <i>bla</i><sub>CTX-M</sub> Carrying Plasmids in Sequential ESBL-<i>E. coli</i> Isolates from Patients with Recurrent Urinary Tract Infections |
| title_short | Identity of <i>bla</i><sub>CTX-M</sub> Carrying Plasmids in Sequential ESBL-<i>E. coli</i> Isolates from Patients with Recurrent Urinary Tract Infections |
| title_sort | identity of i bla i sub ctx m sub carrying plasmids in sequential esbl i e coli i isolates from patients with recurrent urinary tract infections |
| topic | extended-spectrum beta-lactamase (ESBL) recurrent urinary tract infection plasmids horizontal transfer <i>E. coli</i> optical DNA mapping (ODM) |
| url | https://www.mdpi.com/2076-2607/9/6/1138 |
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