Simultaneous Determination of 85 Acid Synthetic Pigments in Sauce by Liquid Chromatography Tandem Mass Spectrometry
A high-throughput liquid chromatography tandem mass spectrometry method was developed for the determination of 85 acid synthetic pigments in sauce. The acid synthetic pigments were extracted with methanol, purified with C18 and diluted with water. Subsequently, the sample was analyzed by liquid chro...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-03-01
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| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021060010 |
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| author | Zihao LIN Xinwu MAO Qingqiong ZHOU Shibo CUI Yuzhong CHEN Ping QI Zhenjie QIAN Wei ZENG |
| author_facet | Zihao LIN Xinwu MAO Qingqiong ZHOU Shibo CUI Yuzhong CHEN Ping QI Zhenjie QIAN Wei ZENG |
| author_sort | Zihao LIN |
| collection | DOAJ |
| description | A high-throughput liquid chromatography tandem mass spectrometry method was developed for the determination of 85 acid synthetic pigments in sauce. The acid synthetic pigments were extracted with methanol, purified with C18 and diluted with water. Subsequently, the sample was analyzed by liquid chromatography-tandem mass spectrometry under multiple reaction monitoring (MRM) mode, and quantified by external standard method. The results showed that the method had a good linearity in the range of 10~1000 ng/mL which correlation coefficients were greater than 0.99. The detection limit of the method was 8.08~885.2 μg/kg, and the quantitative limit was 26.94~2950.7 μg/kg. The average recoveries of synthetic pigments from spiked samples ranged from 70.2% to 116.5%, and the relative standard deviations were between 0.1% and 15.0%. This method was applied to analyze 50 batches of sauce such as tomato sauce and chili sauce, acid red 13 and acid orange II were screened out. The method has strong universality, wide range of detection objects, short analysis time and high sensitivity. It is suitable for the determination of 85 acid synthetic pigments in sauce. |
| first_indexed | 2024-04-11T16:07:42Z |
| format | Article |
| id | doaj.art-b31856dd1a984de8b93ad3b97eeb90c1 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T16:07:42Z |
| publishDate | 2022-03-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-b31856dd1a984de8b93ad3b97eeb90c12022-12-22T04:14:47ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-03-0143527027910.13386/j.issn1002-0306.20210600102021060010-5Simultaneous Determination of 85 Acid Synthetic Pigments in Sauce by Liquid Chromatography Tandem Mass SpectrometryZihao LIN0Xinwu MAO1Qingqiong ZHOU2Shibo CUI3Yuzhong CHEN4Ping QI5Zhenjie QIAN6Wei ZENG7Guangzhou Institute for Food Control, Guangzhou 511400, ChinaGuangzhou Institute for Food Control, Guangzhou 511400, ChinaGuangzhou Institute for Food Control, Guangzhou 511400, ChinaSchool of Food Science & Engineering, South China University of Technology, Guangzhou 510641, ChinaGuangzhou Institute for Food Control, Guangzhou 511400, ChinaGuangzhou Institute for Food Control, Guangzhou 511400, ChinaGuangzhou Institute for Food Control, Guangzhou 511400, ChinaWaters Technologies (Shanghai) Ltd., Shanghai 201203, ChinaA high-throughput liquid chromatography tandem mass spectrometry method was developed for the determination of 85 acid synthetic pigments in sauce. The acid synthetic pigments were extracted with methanol, purified with C18 and diluted with water. Subsequently, the sample was analyzed by liquid chromatography-tandem mass spectrometry under multiple reaction monitoring (MRM) mode, and quantified by external standard method. The results showed that the method had a good linearity in the range of 10~1000 ng/mL which correlation coefficients were greater than 0.99. The detection limit of the method was 8.08~885.2 μg/kg, and the quantitative limit was 26.94~2950.7 μg/kg. The average recoveries of synthetic pigments from spiked samples ranged from 70.2% to 116.5%, and the relative standard deviations were between 0.1% and 15.0%. This method was applied to analyze 50 batches of sauce such as tomato sauce and chili sauce, acid red 13 and acid orange II were screened out. The method has strong universality, wide range of detection objects, short analysis time and high sensitivity. It is suitable for the determination of 85 acid synthetic pigments in sauce.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021060010acid synthetic pigmentsliquid chromatography-tandem mass spectrometrysaucehigh-throughput |
| spellingShingle | Zihao LIN Xinwu MAO Qingqiong ZHOU Shibo CUI Yuzhong CHEN Ping QI Zhenjie QIAN Wei ZENG Simultaneous Determination of 85 Acid Synthetic Pigments in Sauce by Liquid Chromatography Tandem Mass Spectrometry Shipin gongye ke-ji acid synthetic pigments liquid chromatography-tandem mass spectrometry sauce high-throughput |
| title | Simultaneous Determination of 85 Acid Synthetic Pigments in Sauce by Liquid Chromatography Tandem Mass Spectrometry |
| title_full | Simultaneous Determination of 85 Acid Synthetic Pigments in Sauce by Liquid Chromatography Tandem Mass Spectrometry |
| title_fullStr | Simultaneous Determination of 85 Acid Synthetic Pigments in Sauce by Liquid Chromatography Tandem Mass Spectrometry |
| title_full_unstemmed | Simultaneous Determination of 85 Acid Synthetic Pigments in Sauce by Liquid Chromatography Tandem Mass Spectrometry |
| title_short | Simultaneous Determination of 85 Acid Synthetic Pigments in Sauce by Liquid Chromatography Tandem Mass Spectrometry |
| title_sort | simultaneous determination of 85 acid synthetic pigments in sauce by liquid chromatography tandem mass spectrometry |
| topic | acid synthetic pigments liquid chromatography-tandem mass spectrometry sauce high-throughput |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021060010 |
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