Increasing Gene Editing Efficiency via CRISPR/Cas9- or Cas12a-Mediated Knock-In in Primary Human T Cells
T lymphocytes represent a promising target for genome editing. They are primarily modified to recognize and kill tumor cells or to withstand HIV infection. In most studies, T cell genome editing is performed using the CRISPR/Cas technology. Although this technology is easily programmable and widely...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/12/1/119 |
| _version_ | 1797340099243933696 |
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| author | Natalia Kruglova Mikhail Shepelev |
| author_facet | Natalia Kruglova Mikhail Shepelev |
| author_sort | Natalia Kruglova |
| collection | DOAJ |
| description | T lymphocytes represent a promising target for genome editing. They are primarily modified to recognize and kill tumor cells or to withstand HIV infection. In most studies, T cell genome editing is performed using the CRISPR/Cas technology. Although this technology is easily programmable and widely accessible, its efficiency of T cell genome editing was initially low. Several crucial improvements were made in the components of the CRISPR/Cas technology and their delivery methods, as well as in the culturing conditions of T cells, before a reasonable editing level suitable for clinical applications was achieved. In this review, we summarize and describe the aforementioned parameters that affect human T cell editing efficiency using the CRISPR/Cas technology, with a special focus on gene knock-in. |
| first_indexed | 2024-03-08T09:58:05Z |
| format | Article |
| id | doaj.art-b318ab43fa004fa3addc2cc119155c18 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-08T09:58:05Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-b318ab43fa004fa3addc2cc119155c182024-01-29T13:47:18ZengMDPI AGBiomedicines2227-90592024-01-0112111910.3390/biomedicines12010119Increasing Gene Editing Efficiency via CRISPR/Cas9- or Cas12a-Mediated Knock-In in Primary Human T CellsNatalia Kruglova0Mikhail Shepelev1Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology RAS, 119334 Moscow, RussiaCenter for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology RAS, 119334 Moscow, RussiaT lymphocytes represent a promising target for genome editing. They are primarily modified to recognize and kill tumor cells or to withstand HIV infection. In most studies, T cell genome editing is performed using the CRISPR/Cas technology. Although this technology is easily programmable and widely accessible, its efficiency of T cell genome editing was initially low. Several crucial improvements were made in the components of the CRISPR/Cas technology and their delivery methods, as well as in the culturing conditions of T cells, before a reasonable editing level suitable for clinical applications was achieved. In this review, we summarize and describe the aforementioned parameters that affect human T cell editing efficiency using the CRISPR/Cas technology, with a special focus on gene knock-in.https://www.mdpi.com/2227-9059/12/1/119CRISPR/Casgenome editingprimary T cellsknock-inHDRCAR T |
| spellingShingle | Natalia Kruglova Mikhail Shepelev Increasing Gene Editing Efficiency via CRISPR/Cas9- or Cas12a-Mediated Knock-In in Primary Human T Cells Biomedicines CRISPR/Cas genome editing primary T cells knock-in HDR CAR T |
| title | Increasing Gene Editing Efficiency via CRISPR/Cas9- or Cas12a-Mediated Knock-In in Primary Human T Cells |
| title_full | Increasing Gene Editing Efficiency via CRISPR/Cas9- or Cas12a-Mediated Knock-In in Primary Human T Cells |
| title_fullStr | Increasing Gene Editing Efficiency via CRISPR/Cas9- or Cas12a-Mediated Knock-In in Primary Human T Cells |
| title_full_unstemmed | Increasing Gene Editing Efficiency via CRISPR/Cas9- or Cas12a-Mediated Knock-In in Primary Human T Cells |
| title_short | Increasing Gene Editing Efficiency via CRISPR/Cas9- or Cas12a-Mediated Knock-In in Primary Human T Cells |
| title_sort | increasing gene editing efficiency via crispr cas9 or cas12a mediated knock in in primary human t cells |
| topic | CRISPR/Cas genome editing primary T cells knock-in HDR CAR T |
| url | https://www.mdpi.com/2227-9059/12/1/119 |
| work_keys_str_mv | AT nataliakruglova increasinggeneeditingefficiencyviacrisprcas9orcas12amediatedknockininprimaryhumantcells AT mikhailshepelev increasinggeneeditingefficiencyviacrisprcas9orcas12amediatedknockininprimaryhumantcells |