Comparison of Intestinal Microbiota Between Healthy and MMVD Chihuahuas Using 16S rRNA Gene Amplicon Sequencing
Myxomatous mitral valve disease (MMVD) is the most common cause of congestive heart failure in dogs, and although complications of MMVD to the lungs and kidneys have been identified, complications to the gut are less well understood. The intestinal microbiota is an important factor in the gut, and a...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.846492/full |
| _version_ | 1818393641557688320 |
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| author | Ryuji Araki Ryuji Araki Ryuji Araki Koji Iwanaga Kazunori Ueda Ayaka Shima Genki Ishihara Mitsuhiro Aizu Toshiharu Fukayama Mitsuhiro Isaka |
| author_facet | Ryuji Araki Ryuji Araki Ryuji Araki Koji Iwanaga Kazunori Ueda Ayaka Shima Genki Ishihara Mitsuhiro Aizu Toshiharu Fukayama Mitsuhiro Isaka |
| author_sort | Ryuji Araki |
| collection | DOAJ |
| description | Myxomatous mitral valve disease (MMVD) is the most common cause of congestive heart failure in dogs, and although complications of MMVD to the lungs and kidneys have been identified, complications to the gut are less well understood. The intestinal microbiota is an important factor in the gut, and although the association between heart disease and the intestinal microbiota has been shown in human medicine, it is unknown in dogs. The study aimed to evaluate the relationship between MMVD and gut microbiota. A total of 69 healthy Chihuahuas and Chihuahuas with MMVD were evaluated for cardiac health by echocardiography and chest radiography and grouped according to ACVIM guidelines. Fecal samples were collected from all cases and 16S rRNA sequencing was used to reveal the intestinal microbiota. There were significant differences in LA/Ao, LVIDd, E vel, VHS, and VLAS with the severity of ACVIM. On the other hand, there were no significant differences in the diversity and composition of gut microbiota among the groups. The present study did not identify the effects of MMVD on the gut microbiota. |
| first_indexed | 2024-12-14T05:48:33Z |
| format | Article |
| id | doaj.art-b31acc435df34f939ab715ac58aa808f |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-14T05:48:33Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-b31acc435df34f939ab715ac58aa808f2022-12-21T23:14:48ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-03-01910.3389/fvets.2022.846492846492Comparison of Intestinal Microbiota Between Healthy and MMVD Chihuahuas Using 16S rRNA Gene Amplicon SequencingRyuji Araki0Ryuji Araki1Ryuji Araki2Koji Iwanaga3Kazunori Ueda4Ayaka Shima5Genki Ishihara6Mitsuhiro Aizu7Toshiharu Fukayama8Mitsuhiro Isaka9Yokohama Yamate Dog & Cat Medical Center, Yokohama, JapanTokyo Veterinary Cardiology Center, Tokyo, JapanDepartment of Small Animal Clinical Sciences, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, JapanTokyo Veterinary Cardiology Center, Tokyo, JapanYokohama Yamate Dog & Cat Medical Center, Yokohama, JapanAnicom Insurance, Inc., Tokyo, JapanAnicom Insurance, Inc., Tokyo, JapanAnicom Insurance, Inc., Tokyo, JapanAnicom Insurance, Inc., Tokyo, JapanDepartment of Small Animal Clinical Sciences, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, JapanMyxomatous mitral valve disease (MMVD) is the most common cause of congestive heart failure in dogs, and although complications of MMVD to the lungs and kidneys have been identified, complications to the gut are less well understood. The intestinal microbiota is an important factor in the gut, and although the association between heart disease and the intestinal microbiota has been shown in human medicine, it is unknown in dogs. The study aimed to evaluate the relationship between MMVD and gut microbiota. A total of 69 healthy Chihuahuas and Chihuahuas with MMVD were evaluated for cardiac health by echocardiography and chest radiography and grouped according to ACVIM guidelines. Fecal samples were collected from all cases and 16S rRNA sequencing was used to reveal the intestinal microbiota. There were significant differences in LA/Ao, LVIDd, E vel, VHS, and VLAS with the severity of ACVIM. On the other hand, there were no significant differences in the diversity and composition of gut microbiota among the groups. The present study did not identify the effects of MMVD on the gut microbiota.https://www.frontiersin.org/articles/10.3389/fvets.2022.846492/fullChihuahuafractional shorteningintestinal complicationintestinal microbiotamyxomatous mitral valve disease |
| spellingShingle | Ryuji Araki Ryuji Araki Ryuji Araki Koji Iwanaga Kazunori Ueda Ayaka Shima Genki Ishihara Mitsuhiro Aizu Toshiharu Fukayama Mitsuhiro Isaka Comparison of Intestinal Microbiota Between Healthy and MMVD Chihuahuas Using 16S rRNA Gene Amplicon Sequencing Frontiers in Veterinary Science Chihuahua fractional shortening intestinal complication intestinal microbiota myxomatous mitral valve disease |
| title | Comparison of Intestinal Microbiota Between Healthy and MMVD Chihuahuas Using 16S rRNA Gene Amplicon Sequencing |
| title_full | Comparison of Intestinal Microbiota Between Healthy and MMVD Chihuahuas Using 16S rRNA Gene Amplicon Sequencing |
| title_fullStr | Comparison of Intestinal Microbiota Between Healthy and MMVD Chihuahuas Using 16S rRNA Gene Amplicon Sequencing |
| title_full_unstemmed | Comparison of Intestinal Microbiota Between Healthy and MMVD Chihuahuas Using 16S rRNA Gene Amplicon Sequencing |
| title_short | Comparison of Intestinal Microbiota Between Healthy and MMVD Chihuahuas Using 16S rRNA Gene Amplicon Sequencing |
| title_sort | comparison of intestinal microbiota between healthy and mmvd chihuahuas using 16s rrna gene amplicon sequencing |
| topic | Chihuahua fractional shortening intestinal complication intestinal microbiota myxomatous mitral valve disease |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.846492/full |
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