Organ geometry channels reproductive cell fate in the Arabidopsis ovule primordium
In multicellular organisms, sexual reproduction requires the separation of the germline from the soma. In flowering plants, the female germline precursor differentiates as a single spore mother cell (SMC) as the ovule primordium forms. Here, we explored how organ growth contributes to SMC differenti...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2021-05-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/66031 |
| _version_ | 1818026880598540288 |
|---|---|
| author | Elvira Hernandez-Lagana Gabriella Mosca Ethel Mendocilla-Sato Nuno Pires Anja Frey Alejandro Giraldo-Fonseca Caroline Michaud Ueli Grossniklaus Olivier Hamant Christophe Godin Arezki Boudaoud Daniel Grimanelli Daphné Autran Célia Baroux |
| author_facet | Elvira Hernandez-Lagana Gabriella Mosca Ethel Mendocilla-Sato Nuno Pires Anja Frey Alejandro Giraldo-Fonseca Caroline Michaud Ueli Grossniklaus Olivier Hamant Christophe Godin Arezki Boudaoud Daniel Grimanelli Daphné Autran Célia Baroux |
| author_sort | Elvira Hernandez-Lagana |
| collection | DOAJ |
| description | In multicellular organisms, sexual reproduction requires the separation of the germline from the soma. In flowering plants, the female germline precursor differentiates as a single spore mother cell (SMC) as the ovule primordium forms. Here, we explored how organ growth contributes to SMC differentiation. We generated 92 annotated 3D images at cellular resolution in Arabidopsis. We identified the spatio-temporal pattern of cell division that acts in a domain-specific manner as the primordium forms. Tissue growth models uncovered plausible morphogenetic principles involving a spatially confined growth signal, differential mechanical properties, and cell growth anisotropy. Our analysis revealed that SMC characteristics first arise in more than one cell but SMC fate becomes progressively restricted to a single cell during organ growth. Altered primordium geometry coincided with a delay in the fate restriction process in katanin mutants. Altogether, our study suggests that tissue geometry channels reproductive cell fate in the Arabidopsis ovule primordium. |
| first_indexed | 2024-12-10T04:39:02Z |
| format | Article |
| id | doaj.art-b31fd24348464445b8e9342d4481f2d1 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-12-10T04:39:02Z |
| publishDate | 2021-05-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-b31fd24348464445b8e9342d4481f2d12022-12-22T02:01:56ZengeLife Sciences Publications LtdeLife2050-084X2021-05-011010.7554/eLife.66031Organ geometry channels reproductive cell fate in the Arabidopsis ovule primordiumElvira Hernandez-Lagana0https://orcid.org/0000-0002-2645-3783Gabriella Mosca1https://orcid.org/0000-0003-4509-498XEthel Mendocilla-Sato2https://orcid.org/0000-0003-0339-3535Nuno Pires3https://orcid.org/0000-0002-7113-3519Anja Frey4Alejandro Giraldo-Fonseca5Caroline Michaud6https://orcid.org/0000-0002-0620-2442Ueli Grossniklaus7https://orcid.org/0000-0002-0522-8974Olivier Hamant8https://orcid.org/0000-0001-6906-6620Christophe Godin9https://orcid.org/0000-0002-1202-8460Arezki Boudaoud10https://orcid.org/0000-0002-2780-4717Daniel Grimanelli11https://orcid.org/0000-0001-5424-114XDaphné Autran12https://orcid.org/0000-0002-5227-8966Célia Baroux13https://orcid.org/0000-0001-6307-2229DIADE, University of Montpellier, CIRAD, IRD, Montpellier, FranceDepartment of Plant and Microbial Biology and Zurich-Basel Plant Science Center, University of Zürich, Zürich, SwitzerlandDepartment of Plant and Microbial Biology and Zurich-Basel Plant Science Center, University of Zürich, Zürich, SwitzerlandDepartment of Plant and Microbial Biology and Zurich-Basel Plant Science Center, University of Zürich, Zürich, SwitzerlandDepartment of Plant and Microbial Biology and Zurich-Basel Plant Science Center, University of Zürich, Zürich, SwitzerlandDepartment of Plant and Microbial Biology and Zurich-Basel Plant Science Center, University of Zürich, Zürich, SwitzerlandDIADE, University of Montpellier, CIRAD, IRD, Montpellier, FranceDepartment of Plant and Microbial Biology and Zurich-Basel Plant Science Center, University of Zürich, Zürich, SwitzerlandLaboratoire Reproduction et Développement des Plantes, University of Lyon, ENS Lyon, UCB Lyon 1, CNRS, INRAE, INRIA, Lyon, FranceLaboratoire Reproduction et Développement des Plantes, University of Lyon, ENS Lyon, UCB Lyon 1, CNRS, INRAE, INRIA, Lyon, FranceLaboratoire Reproduction et Développement des Plantes, University of Lyon, ENS Lyon, UCB Lyon 1, CNRS, INRAE, INRIA, Lyon, FranceDIADE, University of Montpellier, CIRAD, IRD, Montpellier, FranceDIADE, University of Montpellier, CIRAD, IRD, Montpellier, France; Laboratoire Reproduction et Développement des Plantes, University of Lyon, ENS Lyon, UCB Lyon 1, CNRS, INRAE, INRIA, Lyon, FranceDepartment of Plant and Microbial Biology and Zurich-Basel Plant Science Center, University of Zürich, Zürich, SwitzerlandIn multicellular organisms, sexual reproduction requires the separation of the germline from the soma. In flowering plants, the female germline precursor differentiates as a single spore mother cell (SMC) as the ovule primordium forms. Here, we explored how organ growth contributes to SMC differentiation. We generated 92 annotated 3D images at cellular resolution in Arabidopsis. We identified the spatio-temporal pattern of cell division that acts in a domain-specific manner as the primordium forms. Tissue growth models uncovered plausible morphogenetic principles involving a spatially confined growth signal, differential mechanical properties, and cell growth anisotropy. Our analysis revealed that SMC characteristics first arise in more than one cell but SMC fate becomes progressively restricted to a single cell during organ growth. Altered primordium geometry coincided with a delay in the fate restriction process in katanin mutants. Altogether, our study suggests that tissue geometry channels reproductive cell fate in the Arabidopsis ovule primordium.https://elifesciences.org/articles/66031ovule primordiumgermlinecell fateplasticitygrowthtissue geometry |
| spellingShingle | Elvira Hernandez-Lagana Gabriella Mosca Ethel Mendocilla-Sato Nuno Pires Anja Frey Alejandro Giraldo-Fonseca Caroline Michaud Ueli Grossniklaus Olivier Hamant Christophe Godin Arezki Boudaoud Daniel Grimanelli Daphné Autran Célia Baroux Organ geometry channels reproductive cell fate in the Arabidopsis ovule primordium eLife ovule primordium germline cell fate plasticity growth tissue geometry |
| title | Organ geometry channels reproductive cell fate in the Arabidopsis ovule primordium |
| title_full | Organ geometry channels reproductive cell fate in the Arabidopsis ovule primordium |
| title_fullStr | Organ geometry channels reproductive cell fate in the Arabidopsis ovule primordium |
| title_full_unstemmed | Organ geometry channels reproductive cell fate in the Arabidopsis ovule primordium |
| title_short | Organ geometry channels reproductive cell fate in the Arabidopsis ovule primordium |
| title_sort | organ geometry channels reproductive cell fate in the arabidopsis ovule primordium |
| topic | ovule primordium germline cell fate plasticity growth tissue geometry |
| url | https://elifesciences.org/articles/66031 |
| work_keys_str_mv | AT elvirahernandezlagana organgeometrychannelsreproductivecellfateinthearabidopsisovuleprimordium AT gabriellamosca organgeometrychannelsreproductivecellfateinthearabidopsisovuleprimordium AT ethelmendocillasato organgeometrychannelsreproductivecellfateinthearabidopsisovuleprimordium AT nunopires organgeometrychannelsreproductivecellfateinthearabidopsisovuleprimordium AT anjafrey organgeometrychannelsreproductivecellfateinthearabidopsisovuleprimordium AT alejandrogiraldofonseca organgeometrychannelsreproductivecellfateinthearabidopsisovuleprimordium AT carolinemichaud organgeometrychannelsreproductivecellfateinthearabidopsisovuleprimordium AT ueligrossniklaus organgeometrychannelsreproductivecellfateinthearabidopsisovuleprimordium AT olivierhamant organgeometrychannelsreproductivecellfateinthearabidopsisovuleprimordium AT christophegodin organgeometrychannelsreproductivecellfateinthearabidopsisovuleprimordium AT arezkiboudaoud organgeometrychannelsreproductivecellfateinthearabidopsisovuleprimordium AT danielgrimanelli organgeometrychannelsreproductivecellfateinthearabidopsisovuleprimordium AT daphneautran organgeometrychannelsreproductivecellfateinthearabidopsisovuleprimordium AT celiabaroux organgeometrychannelsreproductivecellfateinthearabidopsisovuleprimordium |