Transcriptome Profile Changes Associated With Heat Shock Reaction in the Entomopathogenic Nematode, Steinernema carpocapsae
The entomopathogenic nematode Steinernema carpocapsae has been used for control of soil insects. However, S. carpocapse is sensitive to environmental factors, particularly temperature. We studied an S. carpocapse group that was shocked with high temperature. We also studied the transcriptome-level r...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-07-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2020.00721/full |
| _version_ | 1811289916621979648 |
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| author | Yi-Fei Xie Yi-Fei Xie Xiu-Dan Wang Wu-Hong Zhong Dao-Hong Zhu Zhen He |
| author_facet | Yi-Fei Xie Yi-Fei Xie Xiu-Dan Wang Wu-Hong Zhong Dao-Hong Zhu Zhen He |
| author_sort | Yi-Fei Xie |
| collection | DOAJ |
| description | The entomopathogenic nematode Steinernema carpocapsae has been used for control of soil insects. However, S. carpocapse is sensitive to environmental factors, particularly temperature. We studied an S. carpocapse group that was shocked with high temperature. We also studied the transcriptome-level responses associated with temperature stress using a BGIseq sequencing platform. We de novo assembled the reads from the treatment and control groups into one transcriptome consisting of 43.9 and 42.9 million clean reads, respectively. Based on the genome database, we aligned the clean reads to the Nr, Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases and analyzed the differentially expressed genes (DEGs). Compared with the control, the heat-shocked group had significant differential expression of the heat shock protein (HSP) family, antioxidase [glutathione S-transferases (GSTs) and superoxide dismutase (SOD)], monooxygenase (P450), and transcription factor genes (DAF-16 and DAF-2). These DEGs were demonstrated to be part of the Longevity pathway and insulin/insulin-like signaling pathway. The results revealed the potential mechanisms, at the transcriptional level, of S. carpocapsae under thermal stress. |
| first_indexed | 2024-04-13T04:03:38Z |
| format | Article |
| id | doaj.art-b31fe5b361444245ad5ed12581979f13 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-13T04:03:38Z |
| publishDate | 2020-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-b31fe5b361444245ad5ed12581979f132022-12-22T03:03:24ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-07-011110.3389/fphys.2020.00721532176Transcriptome Profile Changes Associated With Heat Shock Reaction in the Entomopathogenic Nematode, Steinernema carpocapsaeYi-Fei Xie0Yi-Fei Xie1Xiu-Dan Wang2Wu-Hong Zhong3Dao-Hong Zhu4Zhen He5Hunan Academy of Forestry, Changsha, ChinaCollege of Life Science, Central South University of Forestry and Technology, Changsha, ChinaCollege of Life Science, Central South University of Forestry and Technology, Changsha, ChinaHunan Academy of Forestry, Changsha, ChinaCollege of Life Science, Central South University of Forestry and Technology, Changsha, ChinaHunan Academy of Forestry, Changsha, ChinaThe entomopathogenic nematode Steinernema carpocapsae has been used for control of soil insects. However, S. carpocapse is sensitive to environmental factors, particularly temperature. We studied an S. carpocapse group that was shocked with high temperature. We also studied the transcriptome-level responses associated with temperature stress using a BGIseq sequencing platform. We de novo assembled the reads from the treatment and control groups into one transcriptome consisting of 43.9 and 42.9 million clean reads, respectively. Based on the genome database, we aligned the clean reads to the Nr, Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases and analyzed the differentially expressed genes (DEGs). Compared with the control, the heat-shocked group had significant differential expression of the heat shock protein (HSP) family, antioxidase [glutathione S-transferases (GSTs) and superoxide dismutase (SOD)], monooxygenase (P450), and transcription factor genes (DAF-16 and DAF-2). These DEGs were demonstrated to be part of the Longevity pathway and insulin/insulin-like signaling pathway. The results revealed the potential mechanisms, at the transcriptional level, of S. carpocapsae under thermal stress.https://www.frontiersin.org/article/10.3389/fphys.2020.00721/fullSteinernema carpocapsaetranscriptomeheat shock responsedifferentially expressed geneentomopathogenic nematode |
| spellingShingle | Yi-Fei Xie Yi-Fei Xie Xiu-Dan Wang Wu-Hong Zhong Dao-Hong Zhu Zhen He Transcriptome Profile Changes Associated With Heat Shock Reaction in the Entomopathogenic Nematode, Steinernema carpocapsae Frontiers in Physiology Steinernema carpocapsae transcriptome heat shock response differentially expressed gene entomopathogenic nematode |
| title | Transcriptome Profile Changes Associated With Heat Shock Reaction in the Entomopathogenic Nematode, Steinernema carpocapsae |
| title_full | Transcriptome Profile Changes Associated With Heat Shock Reaction in the Entomopathogenic Nematode, Steinernema carpocapsae |
| title_fullStr | Transcriptome Profile Changes Associated With Heat Shock Reaction in the Entomopathogenic Nematode, Steinernema carpocapsae |
| title_full_unstemmed | Transcriptome Profile Changes Associated With Heat Shock Reaction in the Entomopathogenic Nematode, Steinernema carpocapsae |
| title_short | Transcriptome Profile Changes Associated With Heat Shock Reaction in the Entomopathogenic Nematode, Steinernema carpocapsae |
| title_sort | transcriptome profile changes associated with heat shock reaction in the entomopathogenic nematode steinernema carpocapsae |
| topic | Steinernema carpocapsae transcriptome heat shock response differentially expressed gene entomopathogenic nematode |
| url | https://www.frontiersin.org/article/10.3389/fphys.2020.00721/full |
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