Characterizing the impact of Enterococcus cecorum infection during late embryogenesis on disease progression, cecal microbiome composition, and early performance in broiler chickens
ABSTRACT: Enterococcus cecorum (EC) has been associated with septicemia and early mortality in broiler chickens. There is limited research investigating the pathogenicity of EC field strains obtained from affected birds. The purpose of this study was to evaluate the effect of in-ovo administration i...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123005783 |
| _version_ | 1827793909636399104 |
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| author | Marcela Arango Aaron Forga Jing Liu Guolong Zhang Latasha Gray Randy Moore Makenly Coles Abdiel Atencio Carolina Trujillo Juan David Latorre Guillermo Tellez-Isaias Billy Hargis Danielle Graham |
| author_facet | Marcela Arango Aaron Forga Jing Liu Guolong Zhang Latasha Gray Randy Moore Makenly Coles Abdiel Atencio Carolina Trujillo Juan David Latorre Guillermo Tellez-Isaias Billy Hargis Danielle Graham |
| author_sort | Marcela Arango |
| collection | DOAJ |
| description | ABSTRACT: Enterococcus cecorum (EC) has been associated with septicemia and early mortality in broiler chickens. There is limited research investigating the pathogenicity of EC field strains obtained from affected birds. The purpose of this study was to evaluate the effect of in-ovo administration into the amnion with different EC field isolates at d 18 of embryogenesis (DOE18). In Exp 1, 7 EC field isolates alone or in combination (EC1–EC3, EC4–EC5, EC6, and EC7) were selected based on phenotypic characteristics and evaluated at different concentrations (1 × 102, 1 × 104, and 1 × 106 CFU/200 µL/embryo) to assess the impact on early performance and macroscopic lesions. Three isolates (n = 3; EC2, EC5, EC7) were selected for additional evaluation based on the significant (P < 0.05) BWG reduction (d 0–21) compared to the negative control (NC) and the presence of macroscopic lesions observed during posting sessions at d 14 and d 21. An additional isolate associated with enterococcal spondylitis was included in Exp 2 (EC11B). Treatment groups for Exp 2 include: 1) NC, 2) EC2, 3) EC5, 4) EC7, and 5) EC11B (n = 90–120/embryos/group). Groups 2 to 5 were challenged at 1 × 102 CFU/200 µL/embryo by in-ovo injection into the amnion at DOE18. Chicks were placed in battery cages for the duration of the study (21 d), and pen weights were recorded at d 0, d 7, d 14, and d 21 to calculate average BW and BWG. At d 14 and d 21 posthatch, liver, spleen, free thoracic vertebrae (FTV), and femoral head (FH) were aseptically collected to enumerate Enterococcus spp. using Chromagar Orientation as the selective media. Cecal contents were collected at d 21 to evaluate the effect of EC challenge on the cecal microbiome composition. There was a significant (P < 0.05) reduction in BW at d 21, and BWG from d 14 to 21 and d 0 to 21, for EC7 and EC11B. Enterococcus cecorum was recovered from the FTV of all challenged groups at d 14 and d 21. The most representative lesions were pericarditis, hydropericardium, focal heart necrosis, and FH osteomyelitis. However, lesions were not uniform across challenged groups or ages (d 14 and d 21). Alpha diversity of the cecal contents was markedly lower in EC5 and EC11B compared to all treatment groups suggesting that EC exposure during late embryogenesis affect the cecal microbiome up to 21 d posthatch. Additionally, these results highlight the differences in pathogenicity of EC strains isolated from field cases and suggest that hatchery exposure to EC during late embryogenesis is a potential route of introduction into a flock. |
| first_indexed | 2024-03-11T18:25:19Z |
| format | Article |
| id | doaj.art-b3202cca51484ea5b6adac808a2e1fef |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-11T18:25:19Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-b3202cca51484ea5b6adac808a2e1fef2023-10-14T04:43:57ZengElsevierPoultry Science0032-57912023-11-0110211103059Characterizing the impact of Enterococcus cecorum infection during late embryogenesis on disease progression, cecal microbiome composition, and early performance in broiler chickensMarcela Arango0Aaron Forga1Jing Liu2Guolong Zhang3Latasha Gray4Randy Moore5Makenly Coles6Abdiel Atencio7Carolina Trujillo8Juan David Latorre9Guillermo Tellez-Isaias10Billy Hargis11Danielle Graham12Division of Agriculture, Department of Poultry Science, University of Arkansas, Fayetteville AR 72701, USADivision of Agriculture, Department of Poultry Science, University of Arkansas, Fayetteville AR 72701, USADepartment of Animal Science, Oklahoma State University, Stillwater, OK 74078, USADepartment of Animal Science, Oklahoma State University, Stillwater, OK 74078, USADivision of Agriculture, Department of Poultry Science, University of Arkansas, Fayetteville AR 72701, USAUADA-Veterinary Diagnostic Laboratory, University of Arkansas, Fayetteville AR 72701, USADivision of Agriculture, Department of Poultry Science, University of Arkansas, Fayetteville AR 72701, USADivision of Agriculture, Department of Poultry Science, University of Arkansas, Fayetteville AR 72701, USADivision of Agriculture, Department of Poultry Science, University of Arkansas, Fayetteville AR 72701, USADivision of Agriculture, Department of Poultry Science, University of Arkansas, Fayetteville AR 72701, USADivision of Agriculture, Department of Poultry Science, University of Arkansas, Fayetteville AR 72701, USADivision of Agriculture, Department of Poultry Science, University of Arkansas, Fayetteville AR 72701, USADivision of Agriculture, Department of Poultry Science, University of Arkansas, Fayetteville AR 72701, USA; Corresponding author:ABSTRACT: Enterococcus cecorum (EC) has been associated with septicemia and early mortality in broiler chickens. There is limited research investigating the pathogenicity of EC field strains obtained from affected birds. The purpose of this study was to evaluate the effect of in-ovo administration into the amnion with different EC field isolates at d 18 of embryogenesis (DOE18). In Exp 1, 7 EC field isolates alone or in combination (EC1–EC3, EC4–EC5, EC6, and EC7) were selected based on phenotypic characteristics and evaluated at different concentrations (1 × 102, 1 × 104, and 1 × 106 CFU/200 µL/embryo) to assess the impact on early performance and macroscopic lesions. Three isolates (n = 3; EC2, EC5, EC7) were selected for additional evaluation based on the significant (P < 0.05) BWG reduction (d 0–21) compared to the negative control (NC) and the presence of macroscopic lesions observed during posting sessions at d 14 and d 21. An additional isolate associated with enterococcal spondylitis was included in Exp 2 (EC11B). Treatment groups for Exp 2 include: 1) NC, 2) EC2, 3) EC5, 4) EC7, and 5) EC11B (n = 90–120/embryos/group). Groups 2 to 5 were challenged at 1 × 102 CFU/200 µL/embryo by in-ovo injection into the amnion at DOE18. Chicks were placed in battery cages for the duration of the study (21 d), and pen weights were recorded at d 0, d 7, d 14, and d 21 to calculate average BW and BWG. At d 14 and d 21 posthatch, liver, spleen, free thoracic vertebrae (FTV), and femoral head (FH) were aseptically collected to enumerate Enterococcus spp. using Chromagar Orientation as the selective media. Cecal contents were collected at d 21 to evaluate the effect of EC challenge on the cecal microbiome composition. There was a significant (P < 0.05) reduction in BW at d 21, and BWG from d 14 to 21 and d 0 to 21, for EC7 and EC11B. Enterococcus cecorum was recovered from the FTV of all challenged groups at d 14 and d 21. The most representative lesions were pericarditis, hydropericardium, focal heart necrosis, and FH osteomyelitis. However, lesions were not uniform across challenged groups or ages (d 14 and d 21). Alpha diversity of the cecal contents was markedly lower in EC5 and EC11B compared to all treatment groups suggesting that EC exposure during late embryogenesis affect the cecal microbiome up to 21 d posthatch. Additionally, these results highlight the differences in pathogenicity of EC strains isolated from field cases and suggest that hatchery exposure to EC during late embryogenesis is a potential route of introduction into a flock.http://www.sciencedirect.com/science/article/pii/S0032579123005783Enterococcus cecorumbroilerbacterial infectionenterococci |
| spellingShingle | Marcela Arango Aaron Forga Jing Liu Guolong Zhang Latasha Gray Randy Moore Makenly Coles Abdiel Atencio Carolina Trujillo Juan David Latorre Guillermo Tellez-Isaias Billy Hargis Danielle Graham Characterizing the impact of Enterococcus cecorum infection during late embryogenesis on disease progression, cecal microbiome composition, and early performance in broiler chickens Poultry Science Enterococcus cecorum broiler bacterial infection enterococci |
| title | Characterizing the impact of Enterococcus cecorum infection during late embryogenesis on disease progression, cecal microbiome composition, and early performance in broiler chickens |
| title_full | Characterizing the impact of Enterococcus cecorum infection during late embryogenesis on disease progression, cecal microbiome composition, and early performance in broiler chickens |
| title_fullStr | Characterizing the impact of Enterococcus cecorum infection during late embryogenesis on disease progression, cecal microbiome composition, and early performance in broiler chickens |
| title_full_unstemmed | Characterizing the impact of Enterococcus cecorum infection during late embryogenesis on disease progression, cecal microbiome composition, and early performance in broiler chickens |
| title_short | Characterizing the impact of Enterococcus cecorum infection during late embryogenesis on disease progression, cecal microbiome composition, and early performance in broiler chickens |
| title_sort | characterizing the impact of enterococcus cecorum infection during late embryogenesis on disease progression cecal microbiome composition and early performance in broiler chickens |
| topic | Enterococcus cecorum broiler bacterial infection enterococci |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123005783 |
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