A Comparative Study of Various Pretreatment Approaches for Bio-Ethanol Production from Willow Sawdust, Using Co-Cultures and Mono-Cultures of Different Yeast Strains
The effect of different pretreatment approaches based on alkali (NaOH)/hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) on willow sawdust (WS) biomass, in terms of delignification efficiency, structural changes of lignocellulose and subsequent fermentation toward ethanol, was i...
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MDPI AG
2022-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/4/1344 |
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| author | Imen Ben Atitallah Georgia Antonopoulou Ioanna Ntaikou Amaia Soto Beobide Vassilios Dracopoulos Tahar Mechichi Gerasimos Lyberatos |
| author_facet | Imen Ben Atitallah Georgia Antonopoulou Ioanna Ntaikou Amaia Soto Beobide Vassilios Dracopoulos Tahar Mechichi Gerasimos Lyberatos |
| author_sort | Imen Ben Atitallah |
| collection | DOAJ |
| description | The effect of different pretreatment approaches based on alkali (NaOH)/hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) on willow sawdust (WS) biomass, in terms of delignification efficiency, structural changes of lignocellulose and subsequent fermentation toward ethanol, was investigated. Bioethanol production was carried out using the conventional yeast <i>Saccharomyces cerevisiae,</i> as well as three non-conventional yeasts strains, i.e., <i>Pichia stipitis</i>, <i>Pachysolen tannophilus, Wickerhamomyces anomalus X19</i>, separately and in co-cultures. The experimental results showed that a two-stage pretreatment approach (NaOH (0.5% <i>w</i>/<i>v</i>) for 24 h and H<sub>2</sub>O<sub>2</sub> (0.5% <i>v</i>/<i>v</i>) for 24 h) led to higher delignification (38.3 ± 0.1%) and saccharification efficiency (31.7 ± 0.3%) and higher ethanol concentration and yield. Monocultures of <i>S. cerevisiae</i> or <i>W. anomalus X19</i> and co-cultures with <i>P. stipitis</i> exhibited ethanol yields in the range of 11.67 ± 0.21 to 13.81 ± 0.20 g/100 g total solids (TS). When WS was subjected to H<sub>2</sub>O<sub>2</sub> (0.5% <i>v</i>/<i>v</i>) alone for 24 h, the lowest ethanol yields were observed for all yeast strains, due to the minor impact of this treatment on the main chemical and structural WS characteristics. In order to decide which is the best pretreatment approach, a detailed techno-economical assessment is needed, which will take into account the ethanol yields and the minimum processing cost. |
| first_indexed | 2024-03-09T21:21:05Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T21:21:05Z |
| publishDate | 2022-02-01 |
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| spelling | doaj.art-b320f28c96554d829e6fdfa0ac5e3b862023-11-23T21:22:29ZengMDPI AGMolecules1420-30492022-02-01274134410.3390/molecules27041344A Comparative Study of Various Pretreatment Approaches for Bio-Ethanol Production from Willow Sawdust, Using Co-Cultures and Mono-Cultures of Different Yeast StrainsImen Ben Atitallah0Georgia Antonopoulou1Ioanna Ntaikou2Amaia Soto Beobide3Vassilios Dracopoulos4Tahar Mechichi5Gerasimos Lyberatos6Laboratory of Biochemistry and Enzyme Engineering of Lipases, National School of Engineers of Sfax, University of Sfax, BP 1173, Sfax 3038, TunisiaInstitute of Chemical Engineering Sciences (FORTH/ICE-HT), Stadiou, Platani, GR 26504 Patras, GreeceInstitute of Chemical Engineering Sciences (FORTH/ICE-HT), Stadiou, Platani, GR 26504 Patras, GreeceInstitute of Chemical Engineering Sciences (FORTH/ICE-HT), Stadiou, Platani, GR 26504 Patras, GreeceInstitute of Chemical Engineering Sciences (FORTH/ICE-HT), Stadiou, Platani, GR 26504 Patras, GreeceLaboratory of Biochemistry and Enzyme Engineering of Lipases, National School of Engineers of Sfax, University of Sfax, BP 1173, Sfax 3038, TunisiaInstitute of Chemical Engineering Sciences (FORTH/ICE-HT), Stadiou, Platani, GR 26504 Patras, GreeceThe effect of different pretreatment approaches based on alkali (NaOH)/hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) on willow sawdust (WS) biomass, in terms of delignification efficiency, structural changes of lignocellulose and subsequent fermentation toward ethanol, was investigated. Bioethanol production was carried out using the conventional yeast <i>Saccharomyces cerevisiae,</i> as well as three non-conventional yeasts strains, i.e., <i>Pichia stipitis</i>, <i>Pachysolen tannophilus, Wickerhamomyces anomalus X19</i>, separately and in co-cultures. The experimental results showed that a two-stage pretreatment approach (NaOH (0.5% <i>w</i>/<i>v</i>) for 24 h and H<sub>2</sub>O<sub>2</sub> (0.5% <i>v</i>/<i>v</i>) for 24 h) led to higher delignification (38.3 ± 0.1%) and saccharification efficiency (31.7 ± 0.3%) and higher ethanol concentration and yield. Monocultures of <i>S. cerevisiae</i> or <i>W. anomalus X19</i> and co-cultures with <i>P. stipitis</i> exhibited ethanol yields in the range of 11.67 ± 0.21 to 13.81 ± 0.20 g/100 g total solids (TS). When WS was subjected to H<sub>2</sub>O<sub>2</sub> (0.5% <i>v</i>/<i>v</i>) alone for 24 h, the lowest ethanol yields were observed for all yeast strains, due to the minor impact of this treatment on the main chemical and structural WS characteristics. In order to decide which is the best pretreatment approach, a detailed techno-economical assessment is needed, which will take into account the ethanol yields and the minimum processing cost.https://www.mdpi.com/1420-3049/27/4/1344willow sawdustalkalineperoxidepretreatmentethanol<i>Saccharomyces cerevisiae</i> |
| spellingShingle | Imen Ben Atitallah Georgia Antonopoulou Ioanna Ntaikou Amaia Soto Beobide Vassilios Dracopoulos Tahar Mechichi Gerasimos Lyberatos A Comparative Study of Various Pretreatment Approaches for Bio-Ethanol Production from Willow Sawdust, Using Co-Cultures and Mono-Cultures of Different Yeast Strains Molecules willow sawdust alkaline peroxide pretreatment ethanol <i>Saccharomyces cerevisiae</i> |
| title | A Comparative Study of Various Pretreatment Approaches for Bio-Ethanol Production from Willow Sawdust, Using Co-Cultures and Mono-Cultures of Different Yeast Strains |
| title_full | A Comparative Study of Various Pretreatment Approaches for Bio-Ethanol Production from Willow Sawdust, Using Co-Cultures and Mono-Cultures of Different Yeast Strains |
| title_fullStr | A Comparative Study of Various Pretreatment Approaches for Bio-Ethanol Production from Willow Sawdust, Using Co-Cultures and Mono-Cultures of Different Yeast Strains |
| title_full_unstemmed | A Comparative Study of Various Pretreatment Approaches for Bio-Ethanol Production from Willow Sawdust, Using Co-Cultures and Mono-Cultures of Different Yeast Strains |
| title_short | A Comparative Study of Various Pretreatment Approaches for Bio-Ethanol Production from Willow Sawdust, Using Co-Cultures and Mono-Cultures of Different Yeast Strains |
| title_sort | comparative study of various pretreatment approaches for bio ethanol production from willow sawdust using co cultures and mono cultures of different yeast strains |
| topic | willow sawdust alkaline peroxide pretreatment ethanol <i>Saccharomyces cerevisiae</i> |
| url | https://www.mdpi.com/1420-3049/27/4/1344 |
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