Efficient CRISPR/Cas9-Mediated Gene Knockin in Mouse Hematopoietic Stem and Progenitor Cells

Efficient CRISPR/Cas9-Mediated Gene Knockin in Mouse Hematopoietic Stem and Progenitor Cells

Summary: Mutations accumulating in hematopoietic stem and progenitor cells (HSPCs) during development can cause severe hematological disorders. Modeling these mutations in mice is essential for understanding their functional consequences. Here, we describe an efficient CRISPR/Cas9-based system to kn...

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Bibliographic Details
Main Authors: Ngoc Tung Tran, Thomas Sommermann, Robin Graf, Janine Trombke, Jenniffer Pempe, Kerstin Petsch, Ralf Kühn, Klaus Rajewsky, Van Trung Chu
Format: Article
Language:English
Published: Elsevier 2019-09-01
Series:Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124719311209

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