Characterisation of actinomycetes community from the heavy metals polluted soil
The isolation of actinomycetes was performed from soil samples influenced by car-traffic. The acute toxicity of soil leaches was tested by the help of Microtox® bioassay testing system which uses freeze dried luminescent bacteria Photobacterium phosphoreum as the test organisms. The content of heavy...
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| Format: | Article |
| Language: | English |
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Mendel University Press
2013-01-01
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| Series: | Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis |
| Subjects: | |
| Online Access: | https://acta.mendelu.cz/61/5/1471/ |
| _version_ | 1818296000979140608 |
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| author | Monika Vítězová |
| author_facet | Monika Vítězová |
| author_sort | Monika Vítězová |
| collection | DOAJ |
| description | The isolation of actinomycetes was performed from soil samples influenced by car-traffic. The acute toxicity of soil leaches was tested by the help of Microtox® bioassay testing system which uses freeze dried luminescent bacteria Photobacterium phosphoreum as the test organisms. The content of heavy metals in biomass of soil microorganisms and in whole soil samples was determinate. 115 strains of actinomycetes were isolated and their total numbers in soil samples were estimated. The acute toxicity of soil influenced the total numbers of actinomycetes. By the help of DNA-DNA reassociation procedure the generic diversity of bacteria was estimated. The identification and differentiation of streptomycetes from the total isolated actinomycetes was made using specific morphological criteria and the gas chromatography-fatty acid methyl ester (GC-FAME) analysis. FAME method is adequate only for differentiation of members of genus Streptomyces from other actinomycetes because of their characteristical profile of fatty acids. |
| first_indexed | 2024-12-13T03:56:35Z |
| format | Article |
| id | doaj.art-b324f002964841bfa7f1cf3cf602ccb8 |
| institution | Directory Open Access Journal |
| issn | 1211-8516 2464-8310 |
| language | English |
| last_indexed | 2024-12-13T03:56:35Z |
| publishDate | 2013-01-01 |
| publisher | Mendel University Press |
| record_format | Article |
| series | Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis |
| spelling | doaj.art-b324f002964841bfa7f1cf3cf602ccb82022-12-22T00:00:36ZengMendel University PressActa Universitatis Agriculturae et Silviculturae Mendelianae Brunensis1211-85162464-83102013-01-016151471147810.11118/actaun201361051471Characterisation of actinomycetes community from the heavy metals polluted soilMonika Vítězová0Department of Agrochemistry, Soil Science, Microbiology and Plant Nutrition, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech RepublicThe isolation of actinomycetes was performed from soil samples influenced by car-traffic. The acute toxicity of soil leaches was tested by the help of Microtox® bioassay testing system which uses freeze dried luminescent bacteria Photobacterium phosphoreum as the test organisms. The content of heavy metals in biomass of soil microorganisms and in whole soil samples was determinate. 115 strains of actinomycetes were isolated and their total numbers in soil samples were estimated. The acute toxicity of soil influenced the total numbers of actinomycetes. By the help of DNA-DNA reassociation procedure the generic diversity of bacteria was estimated. The identification and differentiation of streptomycetes from the total isolated actinomycetes was made using specific morphological criteria and the gas chromatography-fatty acid methyl ester (GC-FAME) analysis. FAME method is adequate only for differentiation of members of genus Streptomyces from other actinomycetes because of their characteristical profile of fatty acids.https://acta.mendelu.cz/61/5/1471/soil microorganismsactinomycetesstreptomycetesheavy metalsFAME methodAPI ZYM |
| spellingShingle | Monika Vítězová Characterisation of actinomycetes community from the heavy metals polluted soil Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis soil microorganisms actinomycetes streptomycetes heavy metals FAME method API ZYM |
| title | Characterisation of actinomycetes community from the heavy metals polluted soil |
| title_full | Characterisation of actinomycetes community from the heavy metals polluted soil |
| title_fullStr | Characterisation of actinomycetes community from the heavy metals polluted soil |
| title_full_unstemmed | Characterisation of actinomycetes community from the heavy metals polluted soil |
| title_short | Characterisation of actinomycetes community from the heavy metals polluted soil |
| title_sort | characterisation of actinomycetes community from the heavy metals polluted soil |
| topic | soil microorganisms actinomycetes streptomycetes heavy metals FAME method API ZYM |
| url | https://acta.mendelu.cz/61/5/1471/ |
| work_keys_str_mv | AT monikavitezova characterisationofactinomycetescommunityfromtheheavymetalspollutedsoil |