Epigenetic modifications acetylation and deacetylation play important roles in juvenile hormone action
Abstract Background Epigenetic modifications including DNA methylation and post-translational modifications of histones are known to regulate gene expression. Antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs) mediate transcriptional reprogramming during in...
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| Format: | Article |
| Language: | English |
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BMC
2018-12-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12864-018-5323-4 |
| _version_ | 1828763136546570240 |
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| author | Amit Roy Subba Reddy Palli |
| author_facet | Amit Roy Subba Reddy Palli |
| author_sort | Amit Roy |
| collection | DOAJ |
| description | Abstract Background Epigenetic modifications including DNA methylation and post-translational modifications of histones are known to regulate gene expression. Antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs) mediate transcriptional reprogramming during insect development as shown in Drosophila melanogaster and other insects. Juvenile hormones (JH) play vital roles in the regulation of growth, development, metamorphosis, reproduction and other physiological processes. However, our current understanding of epigenetic regulation of JH action is still limited. Hence, we studied the role of CREB binding protein (CBP, contains HAT domain) and Trichostatin A (TSA, HDAC inhibitor) on JH action. Results Exposure of Tribolium castaneum cells (TcA cells) to JH or TSA caused an increase in expression of Kr-h1 (a known JH-response gene) and 31 or 698 other genes respectively. Knockdown of the gene coding for CBP caused a decrease in the expression of 456 genes including Kr-h1. Interestingly, the expression of several genes coding for transcription factors, nuclear receptors, P450 and fatty acid synthase family members that are known to mediate JH action were affected by CBP knockdown or TSA treatment. Conclusions These data suggest that acetylation and deacetylation mediated by HATs and HDACs play an important role in JH action. |
| first_indexed | 2024-12-11T01:56:00Z |
| format | Article |
| id | doaj.art-b328b4dacc4c4a0cb4da927ed9c2a7c1 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-11T01:56:00Z |
| publishDate | 2018-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-b328b4dacc4c4a0cb4da927ed9c2a7c12022-12-22T01:24:38ZengBMCBMC Genomics1471-21642018-12-0119111510.1186/s12864-018-5323-4Epigenetic modifications acetylation and deacetylation play important roles in juvenile hormone actionAmit Roy0Subba Reddy Palli1Department of Entomology, College of Agriculture, Food and Environment, University of KentuckyDepartment of Entomology, College of Agriculture, Food and Environment, University of KentuckyAbstract Background Epigenetic modifications including DNA methylation and post-translational modifications of histones are known to regulate gene expression. Antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs) mediate transcriptional reprogramming during insect development as shown in Drosophila melanogaster and other insects. Juvenile hormones (JH) play vital roles in the regulation of growth, development, metamorphosis, reproduction and other physiological processes. However, our current understanding of epigenetic regulation of JH action is still limited. Hence, we studied the role of CREB binding protein (CBP, contains HAT domain) and Trichostatin A (TSA, HDAC inhibitor) on JH action. Results Exposure of Tribolium castaneum cells (TcA cells) to JH or TSA caused an increase in expression of Kr-h1 (a known JH-response gene) and 31 or 698 other genes respectively. Knockdown of the gene coding for CBP caused a decrease in the expression of 456 genes including Kr-h1. Interestingly, the expression of several genes coding for transcription factors, nuclear receptors, P450 and fatty acid synthase family members that are known to mediate JH action were affected by CBP knockdown or TSA treatment. Conclusions These data suggest that acetylation and deacetylation mediated by HATs and HDACs play an important role in JH action.http://link.springer.com/article/10.1186/s12864-018-5323-4HATHDACKr-h1FOXO Tribolium and TcA cells |
| spellingShingle | Amit Roy Subba Reddy Palli Epigenetic modifications acetylation and deacetylation play important roles in juvenile hormone action BMC Genomics HAT HDAC Kr-h1 FOXO Tribolium and TcA cells |
| title | Epigenetic modifications acetylation and deacetylation play important roles in juvenile hormone action |
| title_full | Epigenetic modifications acetylation and deacetylation play important roles in juvenile hormone action |
| title_fullStr | Epigenetic modifications acetylation and deacetylation play important roles in juvenile hormone action |
| title_full_unstemmed | Epigenetic modifications acetylation and deacetylation play important roles in juvenile hormone action |
| title_short | Epigenetic modifications acetylation and deacetylation play important roles in juvenile hormone action |
| title_sort | epigenetic modifications acetylation and deacetylation play important roles in juvenile hormone action |
| topic | HAT HDAC Kr-h1 FOXO Tribolium and TcA cells |
| url | http://link.springer.com/article/10.1186/s12864-018-5323-4 |
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