Insulin deficiency and reduced expression of lipogenic enzymes in cardiomyopathic hamster
Evidence is given that the heart of the cardiomyopathic UM-X7.1 hamster has a lipid composition different from that of the same tissue isolated from animals of the Syrian hamster parent strain. Also, noncardiac tissues from cardiomyopathic and healthy hamsters exhibit significant compositional diffe...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2001-01-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520323403 |
| _version_ | 1818728215932305408 |
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| author | Alba Vecchini Luciano Binaglia Marc Bibeau Marilena Minieri Felicia Carotenuto Paolo Di Nardo |
| author_facet | Alba Vecchini Luciano Binaglia Marc Bibeau Marilena Minieri Felicia Carotenuto Paolo Di Nardo |
| author_sort | Alba Vecchini |
| collection | DOAJ |
| description | Evidence is given that the heart of the cardiomyopathic UM-X7.1 hamster has a lipid composition different from that of the same tissue isolated from animals of the Syrian hamster parent strain. Also, noncardiac tissues from cardiomyopathic and healthy hamsters exhibit significant compositional differences. On the basis of these preliminary observations, a comparative study of the hepatic biosynthesis of lipids in cardiomyopathic and healthy Syrian hamsters was undertaken. The results obtained indicate that the cardiomyopathic hamster is characterized by a generalized disturbance of lipid metabolism. In particular, the fatty acid synthase and stearoyl-CoA desaturase activities were significantly lower in the liver of UM-X7.1 hamsters than in age-matched healthy controls fed the same diet. Northern blot analysis of the mRNAs encoding the two enzymatic proteins and the “lipogenic” S14 nuclear protein indicated that the transcription of the respective genes was impaired in UM-X7.1. Short-term dietary manipulations modulated the expression of the above-mentioned genes both in cardiomyopathic and healthy animals. However, dietary carbohydrates were less effective in inducing the expression of lipogenic enzymes in UM-X7.1 liver than healthy controls. The main determinant of the metabolic defect pointed out in the present work appears to be represented by the low insulin level detectable in the plasma of the cardiomyopathic hamster.—Vecchini, A., L. Binaglia, M. Bibeau, M. Minieri, F. Carotenuto, and P. Di Nardo. Insulin deficiency and reduced expression of lipogenic enzymes in cardiomyopathic hamster. J. Lipid Res. 2001. 42: 96–105. |
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| format | Article |
| id | doaj.art-b32c5a847a924a8d821dc7325da53274 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T22:26:28Z |
| publishDate | 2001-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-b32c5a847a924a8d821dc7325da532742022-12-21T21:30:19ZengElsevierJournal of Lipid Research0022-22752001-01-0142196105Insulin deficiency and reduced expression of lipogenic enzymes in cardiomyopathic hamsterAlba Vecchini0Luciano Binaglia1Marc Bibeau2Marilena Minieri3Felicia Carotenuto4Paolo Di Nardo5Institute of Biochemistry, Faculty of Medicine, University of Perugia, 06126 Perugia, ItalyInstitute of Biochemistry, Faculty of Medicine, University of Perugia, 06126 Perugia, ItalyInstitute of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, University of Manitoba, Winnipeg, Manitoba, Canada R2H 2A6Laboratory of Cellular and Molecular Cardiology, Department of Internal Medicine, University of Rome Tor Vergata, 1-00133 Rome, ItalyLaboratory of Cellular and Molecular Cardiology, Department of Internal Medicine, University of Rome Tor Vergata, 1-00133 Rome, ItalyLaboratory of Cellular and Molecular Cardiology, Department of Internal Medicine, University of Rome Tor Vergata, 1-00133 Rome, ItalyEvidence is given that the heart of the cardiomyopathic UM-X7.1 hamster has a lipid composition different from that of the same tissue isolated from animals of the Syrian hamster parent strain. Also, noncardiac tissues from cardiomyopathic and healthy hamsters exhibit significant compositional differences. On the basis of these preliminary observations, a comparative study of the hepatic biosynthesis of lipids in cardiomyopathic and healthy Syrian hamsters was undertaken. The results obtained indicate that the cardiomyopathic hamster is characterized by a generalized disturbance of lipid metabolism. In particular, the fatty acid synthase and stearoyl-CoA desaturase activities were significantly lower in the liver of UM-X7.1 hamsters than in age-matched healthy controls fed the same diet. Northern blot analysis of the mRNAs encoding the two enzymatic proteins and the “lipogenic” S14 nuclear protein indicated that the transcription of the respective genes was impaired in UM-X7.1. Short-term dietary manipulations modulated the expression of the above-mentioned genes both in cardiomyopathic and healthy animals. However, dietary carbohydrates were less effective in inducing the expression of lipogenic enzymes in UM-X7.1 liver than healthy controls. The main determinant of the metabolic defect pointed out in the present work appears to be represented by the low insulin level detectable in the plasma of the cardiomyopathic hamster.—Vecchini, A., L. Binaglia, M. Bibeau, M. Minieri, F. Carotenuto, and P. Di Nardo. Insulin deficiency and reduced expression of lipogenic enzymes in cardiomyopathic hamster. J. Lipid Res. 2001. 42: 96–105.http://www.sciencedirect.com/science/article/pii/S0022227520323403fatty acid synthasestearoyl-CoA desaturasegene expressiondiet |
| spellingShingle | Alba Vecchini Luciano Binaglia Marc Bibeau Marilena Minieri Felicia Carotenuto Paolo Di Nardo Insulin deficiency and reduced expression of lipogenic enzymes in cardiomyopathic hamster Journal of Lipid Research fatty acid synthase stearoyl-CoA desaturase gene expression diet |
| title | Insulin deficiency and reduced expression of lipogenic enzymes in cardiomyopathic hamster |
| title_full | Insulin deficiency and reduced expression of lipogenic enzymes in cardiomyopathic hamster |
| title_fullStr | Insulin deficiency and reduced expression of lipogenic enzymes in cardiomyopathic hamster |
| title_full_unstemmed | Insulin deficiency and reduced expression of lipogenic enzymes in cardiomyopathic hamster |
| title_short | Insulin deficiency and reduced expression of lipogenic enzymes in cardiomyopathic hamster |
| title_sort | insulin deficiency and reduced expression of lipogenic enzymes in cardiomyopathic hamster |
| topic | fatty acid synthase stearoyl-CoA desaturase gene expression diet |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520323403 |
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