First Reported Circulation of Equine Influenza H3N8 Florida Clade 1 Virus in Horses in Italy
Background: Equine influenza (EI) is a highly contagious viral disease of equids characterized by pyrexia and respiratory signs. Like other influenza A viruses, antigenic drift or shift could lead to a vaccine-induced immunity breakdown if vaccine strains are not updated. The aim of this study was t...
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| Format: | Article |
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MDPI AG
2024-02-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/14/4/598 |
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| author | Ida Ricci Silvia Tofani Davide Lelli Giacomo Vincifori Francesca Rosone Andrea Carvelli Elena Lavinia Diaconu Davide La Rocca Giuseppe Manna Samanta Sabatini Donatella Costantini Raffaella Conti Giulia Pacchiarotti Maria Teresa Scicluna |
| author_facet | Ida Ricci Silvia Tofani Davide Lelli Giacomo Vincifori Francesca Rosone Andrea Carvelli Elena Lavinia Diaconu Davide La Rocca Giuseppe Manna Samanta Sabatini Donatella Costantini Raffaella Conti Giulia Pacchiarotti Maria Teresa Scicluna |
| author_sort | Ida Ricci |
| collection | DOAJ |
| description | Background: Equine influenza (EI) is a highly contagious viral disease of equids characterized by pyrexia and respiratory signs. Like other influenza A viruses, antigenic drift or shift could lead to a vaccine-induced immunity breakdown if vaccine strains are not updated. The aim of this study was to genetically characterize EIV strains circulating in Italy, detected in PCR-positive samples collected from suspected cases, especially in the absence of formal active surveillance. Methods: Between February and April 2019, blood samples and nasal swabs collected from each of the 20 symptomatic horses from North and Central Italy were submitted to the National Reference Centre for Equine Diseases in Italy to confirm preliminary analysis performed by other laboratories. Results: None of the sera analysed using haemagglutination inhibition and single radial haemolysis presented a predominant serological reactivity pattern for any antigen employed. All nasal swabs were positive with IAV RRT-PCR. Only one strain, isolated in an embryonated chicken egg from a sample collected from a horse of a stable located in Brescia, Lombardy, was identified as H3N8 Florida lineage clade 1 (FC1). In the constructed phylogenetic trees, this strain is located within the FC1, together with the virus isolated in France in 2018 (MK501761). Conclusions: This study reports the first detection of H3N8 FC1 in Italy, highlighting the importance of monitoring circulating EIV strains to verify the vaccine composition appropriateness for maximum efficacy. |
| first_indexed | 2024-03-07T22:45:56Z |
| format | Article |
| id | doaj.art-b32d8674dcf24e4186135bfb7998e4e2 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-07T22:45:56Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-b32d8674dcf24e4186135bfb7998e4e22024-02-23T15:04:46ZengMDPI AGAnimals2076-26152024-02-0114459810.3390/ani14040598First Reported Circulation of Equine Influenza H3N8 Florida Clade 1 Virus in Horses in ItalyIda Ricci0Silvia Tofani1Davide Lelli2Giacomo Vincifori3Francesca Rosone4Andrea Carvelli5Elena Lavinia Diaconu6Davide La Rocca7Giuseppe Manna8Samanta Sabatini9Donatella Costantini10Raffaella Conti11Giulia Pacchiarotti12Maria Teresa Scicluna13Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini”, Via Bianchi, 9, 25124 Brescia, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, Campo Boario, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, ItalyBackground: Equine influenza (EI) is a highly contagious viral disease of equids characterized by pyrexia and respiratory signs. Like other influenza A viruses, antigenic drift or shift could lead to a vaccine-induced immunity breakdown if vaccine strains are not updated. The aim of this study was to genetically characterize EIV strains circulating in Italy, detected in PCR-positive samples collected from suspected cases, especially in the absence of formal active surveillance. Methods: Between February and April 2019, blood samples and nasal swabs collected from each of the 20 symptomatic horses from North and Central Italy were submitted to the National Reference Centre for Equine Diseases in Italy to confirm preliminary analysis performed by other laboratories. Results: None of the sera analysed using haemagglutination inhibition and single radial haemolysis presented a predominant serological reactivity pattern for any antigen employed. All nasal swabs were positive with IAV RRT-PCR. Only one strain, isolated in an embryonated chicken egg from a sample collected from a horse of a stable located in Brescia, Lombardy, was identified as H3N8 Florida lineage clade 1 (FC1). In the constructed phylogenetic trees, this strain is located within the FC1, together with the virus isolated in France in 2018 (MK501761). Conclusions: This study reports the first detection of H3N8 FC1 in Italy, highlighting the importance of monitoring circulating EIV strains to verify the vaccine composition appropriateness for maximum efficacy.https://www.mdpi.com/2076-2615/14/4/598equine influenzaFlorida clade 1horsegenetic characterization HA and NA |
| spellingShingle | Ida Ricci Silvia Tofani Davide Lelli Giacomo Vincifori Francesca Rosone Andrea Carvelli Elena Lavinia Diaconu Davide La Rocca Giuseppe Manna Samanta Sabatini Donatella Costantini Raffaella Conti Giulia Pacchiarotti Maria Teresa Scicluna First Reported Circulation of Equine Influenza H3N8 Florida Clade 1 Virus in Horses in Italy Animals equine influenza Florida clade 1 horse genetic characterization HA and NA |
| title | First Reported Circulation of Equine Influenza H3N8 Florida Clade 1 Virus in Horses in Italy |
| title_full | First Reported Circulation of Equine Influenza H3N8 Florida Clade 1 Virus in Horses in Italy |
| title_fullStr | First Reported Circulation of Equine Influenza H3N8 Florida Clade 1 Virus in Horses in Italy |
| title_full_unstemmed | First Reported Circulation of Equine Influenza H3N8 Florida Clade 1 Virus in Horses in Italy |
| title_short | First Reported Circulation of Equine Influenza H3N8 Florida Clade 1 Virus in Horses in Italy |
| title_sort | first reported circulation of equine influenza h3n8 florida clade 1 virus in horses in italy |
| topic | equine influenza Florida clade 1 horse genetic characterization HA and NA |
| url | https://www.mdpi.com/2076-2615/14/4/598 |
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