Biomarkers of inflammation in infants with cystic fibrosis
Abstract Background There are urgent needs for clinically relevant biomarkers to identify children with cystic fibrosis (CF) at risk for more progressive lung disease and to serve as outcome measures for clinical trials. Our objective was to investigate three targeted biomarkers in a population of a...
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| Format: | Article |
| Language: | English |
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BMC
2018-01-01
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| Series: | Respiratory Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12931-017-0713-8 |
| _version_ | 1811320212355547136 |
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| author | Theresa A. Laguna Cynthia B. Williams Myra G. Nunez Cole Welchlin-Bradford Catherine E. Moen Cavan S. Reilly Chris H. Wendt |
| author_facet | Theresa A. Laguna Cynthia B. Williams Myra G. Nunez Cole Welchlin-Bradford Catherine E. Moen Cavan S. Reilly Chris H. Wendt |
| author_sort | Theresa A. Laguna |
| collection | DOAJ |
| description | Abstract Background There are urgent needs for clinically relevant biomarkers to identify children with cystic fibrosis (CF) at risk for more progressive lung disease and to serve as outcome measures for clinical trials. Our objective was to investigate three targeted biomarkers in a population of asymptomatic CF infants. Methods Urine, blood and lung function data were collected for 2 years from clinically stable infants diagnosed with CF by newborn screening. A subset of CF infants had bronchoscopy with lavage performed at 6 months and 1 year. Urine was collected quarterly from healthy control infants. Expectorated sputum and urine were collected quarterly for 2 years from clinically stable CF adults. Desmosine, club cell secretory protein (CCSP) and cathepsin B concentrations were measured and compared. Mixed effects models were used to identify associations between biomarker concentrations and clinical characteristics. Receiver operator characteristic curves were generated to investigate the sensitivity and specificity of the biomarkers. Results Urinary cathepsin B was significantly higher in CF infants compared to healthy infants (p = 0.005). CF infant airway and urinary cathepsin B concentrations were significantly lower compared to adult CF subjects (p = 0.002 & p = 0.022, respectively). CF infant airway CCSP was significantly higher than adult CF subjects (p < 0.001). There was a significant correlation between CF infant plasma CCSP and BALF CCSP (p = 0.046). BALF CCSP was negatively associated with IL-8 (p = 0.017). There was no correlation between biomarker concentration and FEV0.5. Conclusions Cathepsin B and CCSP show promise as biomarkers of inflammation in CF infants. Further study is needed. |
| first_indexed | 2024-04-13T12:55:06Z |
| format | Article |
| id | doaj.art-b32dcc037e1940a5b7902b7b7dba7302 |
| institution | Directory Open Access Journal |
| issn | 1465-993X |
| language | English |
| last_indexed | 2024-04-13T12:55:06Z |
| publishDate | 2018-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj.art-b32dcc037e1940a5b7902b7b7dba73022022-12-22T02:46:05ZengBMCRespiratory Research1465-993X2018-01-011911910.1186/s12931-017-0713-8Biomarkers of inflammation in infants with cystic fibrosisTheresa A. Laguna0Cynthia B. Williams1Myra G. Nunez2Cole Welchlin-Bradford3Catherine E. Moen4Cavan S. Reilly5Chris H. Wendt6Minnesota CF Center, Department of Pediatrics, University of Minnesota Masonic Children’s HospitalMinnesota CF Center, Department of Pediatrics, University of Minnesota Masonic Children’s HospitalMinnesota CF Center, Department of Pediatrics, University of Minnesota Masonic Children’s HospitalMinnesota CF Center, Department of Pediatrics, University of Minnesota Masonic Children’s HospitalMinnesota CF Center, Department of Pediatrics, University of Minnesota Masonic Children’s HospitalSchool of Public Health, Division of Biostatistics, University of MinnesotaDepartment of Medicine, Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, University of Minnesota and Veterans Administration Medical CenterAbstract Background There are urgent needs for clinically relevant biomarkers to identify children with cystic fibrosis (CF) at risk for more progressive lung disease and to serve as outcome measures for clinical trials. Our objective was to investigate three targeted biomarkers in a population of asymptomatic CF infants. Methods Urine, blood and lung function data were collected for 2 years from clinically stable infants diagnosed with CF by newborn screening. A subset of CF infants had bronchoscopy with lavage performed at 6 months and 1 year. Urine was collected quarterly from healthy control infants. Expectorated sputum and urine were collected quarterly for 2 years from clinically stable CF adults. Desmosine, club cell secretory protein (CCSP) and cathepsin B concentrations were measured and compared. Mixed effects models were used to identify associations between biomarker concentrations and clinical characteristics. Receiver operator characteristic curves were generated to investigate the sensitivity and specificity of the biomarkers. Results Urinary cathepsin B was significantly higher in CF infants compared to healthy infants (p = 0.005). CF infant airway and urinary cathepsin B concentrations were significantly lower compared to adult CF subjects (p = 0.002 & p = 0.022, respectively). CF infant airway CCSP was significantly higher than adult CF subjects (p < 0.001). There was a significant correlation between CF infant plasma CCSP and BALF CCSP (p = 0.046). BALF CCSP was negatively associated with IL-8 (p = 0.017). There was no correlation between biomarker concentration and FEV0.5. Conclusions Cathepsin B and CCSP show promise as biomarkers of inflammation in CF infants. Further study is needed.http://link.springer.com/article/10.1186/s12931-017-0713-8InflammationLung functionInfectionPediatricsBronchoalveolar lavage fluid |
| spellingShingle | Theresa A. Laguna Cynthia B. Williams Myra G. Nunez Cole Welchlin-Bradford Catherine E. Moen Cavan S. Reilly Chris H. Wendt Biomarkers of inflammation in infants with cystic fibrosis Respiratory Research Inflammation Lung function Infection Pediatrics Bronchoalveolar lavage fluid |
| title | Biomarkers of inflammation in infants with cystic fibrosis |
| title_full | Biomarkers of inflammation in infants with cystic fibrosis |
| title_fullStr | Biomarkers of inflammation in infants with cystic fibrosis |
| title_full_unstemmed | Biomarkers of inflammation in infants with cystic fibrosis |
| title_short | Biomarkers of inflammation in infants with cystic fibrosis |
| title_sort | biomarkers of inflammation in infants with cystic fibrosis |
| topic | Inflammation Lung function Infection Pediatrics Bronchoalveolar lavage fluid |
| url | http://link.springer.com/article/10.1186/s12931-017-0713-8 |
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