Effect of Etching Procedures on the Adhesion of Biofilm-Coated Dentin
Oral biofilms coat all surfaces in the oral cavity including the exposed dentin surface. This study aimed to investigate biofilm removal by acid etching procedures and the effects of the residual biofilm on dentin surfaces on composite–dentin adhesion. Dentin discs were assigned to five groups: no b...
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2020-06-01
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| Online Access: | https://www.mdpi.com/1996-1944/13/12/2762 |
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| author | Bo-Kyung Jeon Chang-Ha Lee A Reum Kim Seung Hyun Han Hyun-Jung Kim Sibel A. Antonson Sun-Young Kim |
| author_facet | Bo-Kyung Jeon Chang-Ha Lee A Reum Kim Seung Hyun Han Hyun-Jung Kim Sibel A. Antonson Sun-Young Kim |
| author_sort | Bo-Kyung Jeon |
| collection | DOAJ |
| description | Oral biofilms coat all surfaces in the oral cavity including the exposed dentin surface. This study aimed to investigate biofilm removal by acid etching procedures and the effects of the residual biofilm on dentin surfaces on composite–dentin adhesion. Dentin discs were assigned to five groups: no biofilm formation (C); biofilm formation and no surface treatment (BF); biofilm formation and acid etching (BF-E); biofilm formation and acid etching followed by chlorhexidine soaking (BF-EC); and biofilm formation and rubbing with pumice, followed by acid etching (BF-RE). Biofilms were formed on saliva-precoated dentin discs by soaking the discs in <i>Streptococcus mutans</i> (<i>S. mutans</i>) suspension. Biofilm removal from the dentin surface was evaluated quantitatively and qualitatively by confocal laser scanning microscopy and scanning electron microscopy, respectively. To compare the bond strength of the biofilm-coated dentin discs with the surface treatments, specimens were assigned to four groups: no biofilm formation and acid etching (C-E); BF-E; BF-EC; and BF-RE. Assessments of the micro-shear bond strength and subsequent failure modes were performed. BF-E and BF-EC did not remove the biofilm, whereas BF-RE partially removed the biofilm attached to the dentin (<i>p</i> < 0.05). The bond strength of BF-RE was significantly higher than those of BF-E and BF-EC, but lower than that of C-E (<i>p</i> < 0.05). In conclusion, mechanical biofilm removal is recommended before etching procedures to enhance adhesion to the biofilm-coated dentin. |
| first_indexed | 2024-03-10T19:04:26Z |
| format | Article |
| id | doaj.art-b32e83931e1b475e81ad034feb11f898 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T19:04:26Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-b32e83931e1b475e81ad034feb11f8982023-11-20T04:13:28ZengMDPI AGMaterials1996-19442020-06-011312276210.3390/ma13122762Effect of Etching Procedures on the Adhesion of Biofilm-Coated DentinBo-Kyung Jeon0Chang-Ha Lee1A Reum Kim2Seung Hyun Han3Hyun-Jung Kim4Sibel A. Antonson5Sun-Young Kim6Department of Conservative Dentistry and Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, KoreaDepartment of Conservative Dentistry and Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, KoreaDepartment of Oral Microbiology and Immunology, School of Dentistry, Seoul National University, Seoul 08826, KoreaDepartment of Oral Microbiology and Immunology, School of Dentistry, Seoul National University, Seoul 08826, KoreaDepartment of Conservative Dentistry, Kyung Hee University Dental Hospital, Seoul 02454, KoreaDepartment of Cariology and Restorative Dentistry, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, FL 33314, USADepartment of Conservative Dentistry and Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, KoreaOral biofilms coat all surfaces in the oral cavity including the exposed dentin surface. This study aimed to investigate biofilm removal by acid etching procedures and the effects of the residual biofilm on dentin surfaces on composite–dentin adhesion. Dentin discs were assigned to five groups: no biofilm formation (C); biofilm formation and no surface treatment (BF); biofilm formation and acid etching (BF-E); biofilm formation and acid etching followed by chlorhexidine soaking (BF-EC); and biofilm formation and rubbing with pumice, followed by acid etching (BF-RE). Biofilms were formed on saliva-precoated dentin discs by soaking the discs in <i>Streptococcus mutans</i> (<i>S. mutans</i>) suspension. Biofilm removal from the dentin surface was evaluated quantitatively and qualitatively by confocal laser scanning microscopy and scanning electron microscopy, respectively. To compare the bond strength of the biofilm-coated dentin discs with the surface treatments, specimens were assigned to four groups: no biofilm formation and acid etching (C-E); BF-E; BF-EC; and BF-RE. Assessments of the micro-shear bond strength and subsequent failure modes were performed. BF-E and BF-EC did not remove the biofilm, whereas BF-RE partially removed the biofilm attached to the dentin (<i>p</i> < 0.05). The bond strength of BF-RE was significantly higher than those of BF-E and BF-EC, but lower than that of C-E (<i>p</i> < 0.05). In conclusion, mechanical biofilm removal is recommended before etching procedures to enhance adhesion to the biofilm-coated dentin.https://www.mdpi.com/1996-1944/13/12/2762acid etchingbiofilmconfocal laser scanning microscopymicro-shear bond strength<i>S. mutans</i> |
| spellingShingle | Bo-Kyung Jeon Chang-Ha Lee A Reum Kim Seung Hyun Han Hyun-Jung Kim Sibel A. Antonson Sun-Young Kim Effect of Etching Procedures on the Adhesion of Biofilm-Coated Dentin Materials acid etching biofilm confocal laser scanning microscopy micro-shear bond strength <i>S. mutans</i> |
| title | Effect of Etching Procedures on the Adhesion of Biofilm-Coated Dentin |
| title_full | Effect of Etching Procedures on the Adhesion of Biofilm-Coated Dentin |
| title_fullStr | Effect of Etching Procedures on the Adhesion of Biofilm-Coated Dentin |
| title_full_unstemmed | Effect of Etching Procedures on the Adhesion of Biofilm-Coated Dentin |
| title_short | Effect of Etching Procedures on the Adhesion of Biofilm-Coated Dentin |
| title_sort | effect of etching procedures on the adhesion of biofilm coated dentin |
| topic | acid etching biofilm confocal laser scanning microscopy micro-shear bond strength <i>S. mutans</i> |
| url | https://www.mdpi.com/1996-1944/13/12/2762 |
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