Characterizations of a Class-I BASIC PENTACYSTEINE Gene Reveal Conserved Roles in the Transcriptional Repression of Genes Involved in Seed Development
The developmental regulation of flower organs involves the spatio-temporal regulation of floral homeotic genes. <i>BASIC PENTACYSTEINE</i> genes are plant-specific transcription factors that is involved in many aspects of plant development through gene transcriptional regulation. Althoug...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
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| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/44/9/278 |
| Summary: | The developmental regulation of flower organs involves the spatio-temporal regulation of floral homeotic genes. <i>BASIC PENTACYSTEINE</i> genes are plant-specific transcription factors that is involved in many aspects of plant development through gene transcriptional regulation. Although studies have shown that the <i>BPC</i> genes are involved in the developmental regulation of flower organs, little is known about their role in the formation of double-flower due. Here we characterized a Class I BPC gene (<i>CjBPC1</i>) from an ornamental flower—<i>Camellia japonica</i>. We showed that <i>CjBPC1</i> is highly expressed in the central whorls of flowers in both single and doubled varieties. Overexpression of <i>CjBPC1</i> in <i>Arabidopsis thaliana</i> caused severe defects in siliques and seeds. We found that genes involved in ovule and seed development, including <i>SEEDSTICK</i>, <i>LEAFY COTYLEDON2</i>, <i>ABSCISIC ACID INSENSITIVE 3</i> and <i>FUSCA3</i>, were significantly down-regulated in transgenic lines. We showed that the histone 3 lysine 27 methylation levels of these downstream genes were enhanced in the transgenic plants, indicating conserved roles of <i>CjBPC1</i> in recruiting the Polycomb Repression Complex for gene suppression. |
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| ISSN: | 1467-3037 1467-3045 |