Characterizations of a Class-I BASIC PENTACYSTEINE Gene Reveal Conserved Roles in the Transcriptional Repression of Genes Involved in Seed Development
The developmental regulation of flower organs involves the spatio-temporal regulation of floral homeotic genes. <i>BASIC PENTACYSTEINE</i> genes are plant-specific transcription factors that is involved in many aspects of plant development through gene transcriptional regulation. Althoug...
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MDPI AG
2022-09-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/44/9/278 |
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| author | Xianjin Ma Yifan Yu Zhikang Hu Hu Huang Sijia Li Hengfu Yin |
| author_facet | Xianjin Ma Yifan Yu Zhikang Hu Hu Huang Sijia Li Hengfu Yin |
| author_sort | Xianjin Ma |
| collection | DOAJ |
| description | The developmental regulation of flower organs involves the spatio-temporal regulation of floral homeotic genes. <i>BASIC PENTACYSTEINE</i> genes are plant-specific transcription factors that is involved in many aspects of plant development through gene transcriptional regulation. Although studies have shown that the <i>BPC</i> genes are involved in the developmental regulation of flower organs, little is known about their role in the formation of double-flower due. Here we characterized a Class I BPC gene (<i>CjBPC1</i>) from an ornamental flower—<i>Camellia japonica</i>. We showed that <i>CjBPC1</i> is highly expressed in the central whorls of flowers in both single and doubled varieties. Overexpression of <i>CjBPC1</i> in <i>Arabidopsis thaliana</i> caused severe defects in siliques and seeds. We found that genes involved in ovule and seed development, including <i>SEEDSTICK</i>, <i>LEAFY COTYLEDON2</i>, <i>ABSCISIC ACID INSENSITIVE 3</i> and <i>FUSCA3</i>, were significantly down-regulated in transgenic lines. We showed that the histone 3 lysine 27 methylation levels of these downstream genes were enhanced in the transgenic plants, indicating conserved roles of <i>CjBPC1</i> in recruiting the Polycomb Repression Complex for gene suppression. |
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| language | English |
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| series | Current Issues in Molecular Biology |
| spelling | doaj.art-b32eaacc38e14dfcb1d300befb003ad92023-11-23T15:39:12ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452022-09-014494059406910.3390/cimb44090278Characterizations of a Class-I BASIC PENTACYSTEINE Gene Reveal Conserved Roles in the Transcriptional Repression of Genes Involved in Seed DevelopmentXianjin Ma0Yifan Yu1Zhikang Hu2Hu Huang3Sijia Li4Hengfu Yin5State Key Laboratory of Tree Genetics and Breeding, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, ChinaState Key Laboratory of Tree Genetics and Breeding, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, ChinaState Key Laboratory of Tree Genetics and Breeding, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, ChinaState Key Laboratory of Tree Genetics and Breeding, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, ChinaState Key Laboratory of Tree Genetics and Breeding, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, ChinaState Key Laboratory of Tree Genetics and Breeding, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, ChinaThe developmental regulation of flower organs involves the spatio-temporal regulation of floral homeotic genes. <i>BASIC PENTACYSTEINE</i> genes are plant-specific transcription factors that is involved in many aspects of plant development through gene transcriptional regulation. Although studies have shown that the <i>BPC</i> genes are involved in the developmental regulation of flower organs, little is known about their role in the formation of double-flower due. Here we characterized a Class I BPC gene (<i>CjBPC1</i>) from an ornamental flower—<i>Camellia japonica</i>. We showed that <i>CjBPC1</i> is highly expressed in the central whorls of flowers in both single and doubled varieties. Overexpression of <i>CjBPC1</i> in <i>Arabidopsis thaliana</i> caused severe defects in siliques and seeds. We found that genes involved in ovule and seed development, including <i>SEEDSTICK</i>, <i>LEAFY COTYLEDON2</i>, <i>ABSCISIC ACID INSENSITIVE 3</i> and <i>FUSCA3</i>, were significantly down-regulated in transgenic lines. We showed that the histone 3 lysine 27 methylation levels of these downstream genes were enhanced in the transgenic plants, indicating conserved roles of <i>CjBPC1</i> in recruiting the Polycomb Repression Complex for gene suppression.https://www.mdpi.com/1467-3045/44/9/278BPC transcription factorflower developmenthistone methylationovuleseed |
| spellingShingle | Xianjin Ma Yifan Yu Zhikang Hu Hu Huang Sijia Li Hengfu Yin Characterizations of a Class-I BASIC PENTACYSTEINE Gene Reveal Conserved Roles in the Transcriptional Repression of Genes Involved in Seed Development Current Issues in Molecular Biology BPC transcription factor flower development histone methylation ovule seed |
| title | Characterizations of a Class-I BASIC PENTACYSTEINE Gene Reveal Conserved Roles in the Transcriptional Repression of Genes Involved in Seed Development |
| title_full | Characterizations of a Class-I BASIC PENTACYSTEINE Gene Reveal Conserved Roles in the Transcriptional Repression of Genes Involved in Seed Development |
| title_fullStr | Characterizations of a Class-I BASIC PENTACYSTEINE Gene Reveal Conserved Roles in the Transcriptional Repression of Genes Involved in Seed Development |
| title_full_unstemmed | Characterizations of a Class-I BASIC PENTACYSTEINE Gene Reveal Conserved Roles in the Transcriptional Repression of Genes Involved in Seed Development |
| title_short | Characterizations of a Class-I BASIC PENTACYSTEINE Gene Reveal Conserved Roles in the Transcriptional Repression of Genes Involved in Seed Development |
| title_sort | characterizations of a class i basic pentacysteine gene reveal conserved roles in the transcriptional repression of genes involved in seed development |
| topic | BPC transcription factor flower development histone methylation ovule seed |
| url | https://www.mdpi.com/1467-3045/44/9/278 |
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