REBELOTE, a regulator of floral determinacy in Arabidopsis thaliana, interacts with both nucleolar and nucleoplasmic proteins
The nucleoplasm and nucleolus are the two main territories of the nucleus. While specific functions are associated with each of these territories (such as mRNA synthesis in the nucleoplasm and ribosomal rRNA synthesis in the nucleolus), some proteins are known to be located in both. Here, we investi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-10-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.12504 |
| _version_ | 1828330552361484288 |
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| author | Stève de Bossoreille Patrice Morel Christophe Trehin Ioan Negrutiu |
| author_facet | Stève de Bossoreille Patrice Morel Christophe Trehin Ioan Negrutiu |
| author_sort | Stève de Bossoreille |
| collection | DOAJ |
| description | The nucleoplasm and nucleolus are the two main territories of the nucleus. While specific functions are associated with each of these territories (such as mRNA synthesis in the nucleoplasm and ribosomal rRNA synthesis in the nucleolus), some proteins are known to be located in both. Here, we investigated the molecular function of REBELOTE (RBL), an Arabidopsis thaliana protein previously characterized as a regulator of floral meristem termination. We show that RBL displays a dual localization, in the nucleolus and nucleoplasm. Moreover, we used direct and global approaches to demonstrate that RBL interacts with nucleic acid‐binding proteins. It binds to the NOC proteins SWA2, AtNOC2 and AtNOC3 in both the nucleolus and nucleoplasm, and also to OBE1 and VFP3/ENAP1. Taking into account the identities of these RBL interactors, we hypothesize that RBL acts both in ribosomal biogenesis and in the regulation of gene expression. |
| first_indexed | 2024-04-13T20:40:56Z |
| format | Article |
| id | doaj.art-b3349033d6964174b1417d59e3b212ff |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-04-13T20:40:56Z |
| publishDate | 2018-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-b3349033d6964174b1417d59e3b212ff2022-12-22T02:30:51ZengWileyFEBS Open Bio2211-54632018-10-018101636164810.1002/2211-5463.12504REBELOTE, a regulator of floral determinacy in Arabidopsis thaliana, interacts with both nucleolar and nucleoplasmic proteinsStève de Bossoreille0Patrice Morel1Christophe Trehin2Ioan Negrutiu3Laboratoire Reproduction et Développement des Plantes Univ Lyon ENS de Lyon UCB Lyon 1 CNRS, INRA Lyon FranceLaboratoire Reproduction et Développement des Plantes Univ Lyon ENS de Lyon UCB Lyon 1 CNRS, INRA Lyon FranceLaboratoire Reproduction et Développement des Plantes Univ Lyon ENS de Lyon UCB Lyon 1 CNRS, INRA Lyon FranceLaboratoire Reproduction et Développement des Plantes Univ Lyon ENS de Lyon UCB Lyon 1 CNRS, INRA Lyon FranceThe nucleoplasm and nucleolus are the two main territories of the nucleus. While specific functions are associated with each of these territories (such as mRNA synthesis in the nucleoplasm and ribosomal rRNA synthesis in the nucleolus), some proteins are known to be located in both. Here, we investigated the molecular function of REBELOTE (RBL), an Arabidopsis thaliana protein previously characterized as a regulator of floral meristem termination. We show that RBL displays a dual localization, in the nucleolus and nucleoplasm. Moreover, we used direct and global approaches to demonstrate that RBL interacts with nucleic acid‐binding proteins. It binds to the NOC proteins SWA2, AtNOC2 and AtNOC3 in both the nucleolus and nucleoplasm, and also to OBE1 and VFP3/ENAP1. Taking into account the identities of these RBL interactors, we hypothesize that RBL acts both in ribosomal biogenesis and in the regulation of gene expression.https://doi.org/10.1002/2211-5463.12504Arabidopsis thalianaENAP1NOCnucleolusnucleoplasmOBE1 |
| spellingShingle | Stève de Bossoreille Patrice Morel Christophe Trehin Ioan Negrutiu REBELOTE, a regulator of floral determinacy in Arabidopsis thaliana, interacts with both nucleolar and nucleoplasmic proteins FEBS Open Bio Arabidopsis thaliana ENAP1 NOC nucleolus nucleoplasm OBE1 |
| title | REBELOTE, a regulator of floral determinacy in Arabidopsis thaliana, interacts with both nucleolar and nucleoplasmic proteins |
| title_full | REBELOTE, a regulator of floral determinacy in Arabidopsis thaliana, interacts with both nucleolar and nucleoplasmic proteins |
| title_fullStr | REBELOTE, a regulator of floral determinacy in Arabidopsis thaliana, interacts with both nucleolar and nucleoplasmic proteins |
| title_full_unstemmed | REBELOTE, a regulator of floral determinacy in Arabidopsis thaliana, interacts with both nucleolar and nucleoplasmic proteins |
| title_short | REBELOTE, a regulator of floral determinacy in Arabidopsis thaliana, interacts with both nucleolar and nucleoplasmic proteins |
| title_sort | rebelote a regulator of floral determinacy in arabidopsis thaliana interacts with both nucleolar and nucleoplasmic proteins |
| topic | Arabidopsis thaliana ENAP1 NOC nucleolus nucleoplasm OBE1 |
| url | https://doi.org/10.1002/2211-5463.12504 |
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