Direct-Current Electrical Field Stimulation of Patient-Derived Colorectal Cancer Cells
Several cues for a directional migration of colorectal cancer cells were identified as being crucial in tumor progression. However, galvanotaxis, the directional migration in direct-current electrical fields, has not been investigated so far. Therefore, we asked whether direct-current electrical fie...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/12/7/1032 |
| _version_ | 1827733889847656448 |
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| author | Falko Lange Katrin Porath Tina Sellmann Anne Einsle Robert Jaster Michael Linnebacher Rüdiger Köhling Timo Kirschstein |
| author_facet | Falko Lange Katrin Porath Tina Sellmann Anne Einsle Robert Jaster Michael Linnebacher Rüdiger Köhling Timo Kirschstein |
| author_sort | Falko Lange |
| collection | DOAJ |
| description | Several cues for a directional migration of colorectal cancer cells were identified as being crucial in tumor progression. However, galvanotaxis, the directional migration in direct-current electrical fields, has not been investigated so far. Therefore, we asked whether direct-current electrical fields could be used to mobilize colorectal cancer cells along field vectors. For this purpose, five patient-derived low-passage cell lines were exposed to field strengths of 150–250 V/m in vitro, and migration along the field vectors was investigated. To further study the role of voltage-gated calcium channels on galvanotaxis and intracellular signaling pathways that are associated with migration of colorectal cancer cells, the cultures were exposed to selective inhibitors. In three out of five colorectal cancer cell lines, we found a preferred cathodal migration. The cellular integrity of the cells was not impaired by exposure of the cells to the selected field strengths. Galvanotaxis was sensitive to inhibition of voltage-gated calcium channels. Furthermore, signaling pathways such as AKT and MEK, but not STAT3, were also found to contribute to galvanotaxis in our in vitro model system. Overall, we identify electrical fields as an important contributor to the directional migration of colorectal cancer cells. |
| first_indexed | 2024-03-11T01:16:56Z |
| format | Article |
| id | doaj.art-b33524b735304477832211c4cfb216e8 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-11T01:16:56Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-b33524b735304477832211c4cfb216e82023-11-18T18:24:38ZengMDPI AGBiology2079-77372023-07-01127103210.3390/biology12071032Direct-Current Electrical Field Stimulation of Patient-Derived Colorectal Cancer CellsFalko Lange0Katrin Porath1Tina Sellmann2Anne Einsle3Robert Jaster4Michael Linnebacher5Rüdiger Köhling6Timo Kirschstein7Oscar-Langendorff-Institute of Physiology, Rostock University Medical Center, 18057 Rostock, GermanyOscar-Langendorff-Institute of Physiology, Rostock University Medical Center, 18057 Rostock, GermanyOscar-Langendorff-Institute of Physiology, Rostock University Medical Center, 18057 Rostock, GermanyOscar-Langendorff-Institute of Physiology, Rostock University Medical Center, 18057 Rostock, GermanyDivision of Gastroenterology and Endocrinology, Department of Medicine II, Rostock University Medical Center, 18057 Rostock, GermanyMolecular Oncology and Immunotherapy, Clinic of General Surgery, Rostock University Medical Center, 18057 Rostock, GermanyOscar-Langendorff-Institute of Physiology, Rostock University Medical Center, 18057 Rostock, GermanyOscar-Langendorff-Institute of Physiology, Rostock University Medical Center, 18057 Rostock, GermanySeveral cues for a directional migration of colorectal cancer cells were identified as being crucial in tumor progression. However, galvanotaxis, the directional migration in direct-current electrical fields, has not been investigated so far. Therefore, we asked whether direct-current electrical fields could be used to mobilize colorectal cancer cells along field vectors. For this purpose, five patient-derived low-passage cell lines were exposed to field strengths of 150–250 V/m in vitro, and migration along the field vectors was investigated. To further study the role of voltage-gated calcium channels on galvanotaxis and intracellular signaling pathways that are associated with migration of colorectal cancer cells, the cultures were exposed to selective inhibitors. In three out of five colorectal cancer cell lines, we found a preferred cathodal migration. The cellular integrity of the cells was not impaired by exposure of the cells to the selected field strengths. Galvanotaxis was sensitive to inhibition of voltage-gated calcium channels. Furthermore, signaling pathways such as AKT and MEK, but not STAT3, were also found to contribute to galvanotaxis in our in vitro model system. Overall, we identify electrical fields as an important contributor to the directional migration of colorectal cancer cells.https://www.mdpi.com/2079-7737/12/7/1032galvanotaxisdirect-current electrical fieldcolorectal cancerpatient-derived low-passage cell linescalcium influxPI3K/AKT pathway |
| spellingShingle | Falko Lange Katrin Porath Tina Sellmann Anne Einsle Robert Jaster Michael Linnebacher Rüdiger Köhling Timo Kirschstein Direct-Current Electrical Field Stimulation of Patient-Derived Colorectal Cancer Cells Biology galvanotaxis direct-current electrical field colorectal cancer patient-derived low-passage cell lines calcium influx PI3K/AKT pathway |
| title | Direct-Current Electrical Field Stimulation of Patient-Derived Colorectal Cancer Cells |
| title_full | Direct-Current Electrical Field Stimulation of Patient-Derived Colorectal Cancer Cells |
| title_fullStr | Direct-Current Electrical Field Stimulation of Patient-Derived Colorectal Cancer Cells |
| title_full_unstemmed | Direct-Current Electrical Field Stimulation of Patient-Derived Colorectal Cancer Cells |
| title_short | Direct-Current Electrical Field Stimulation of Patient-Derived Colorectal Cancer Cells |
| title_sort | direct current electrical field stimulation of patient derived colorectal cancer cells |
| topic | galvanotaxis direct-current electrical field colorectal cancer patient-derived low-passage cell lines calcium influx PI3K/AKT pathway |
| url | https://www.mdpi.com/2079-7737/12/7/1032 |
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