Pathways Activated by Infected and Bystander Chondrocytes in Response to Ross River Virus Infection
Old world alphaviruses, such as Ross River virus (RRV), cause debilitating arthralgia during acute and chronic stages of the disease. RRV-induced cartilage degradation has been implicated as a cause of joint pain felt by RRV patients. Chondrocytes are a major cell type of cartilage and are involved...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/1/136 |
| _version_ | 1827621358246297600 |
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| author | Elisa X. Y. Lim Julie A. Webster Penny A. Rudd Lara J. Herrero |
| author_facet | Elisa X. Y. Lim Julie A. Webster Penny A. Rudd Lara J. Herrero |
| author_sort | Elisa X. Y. Lim |
| collection | DOAJ |
| description | Old world alphaviruses, such as Ross River virus (RRV), cause debilitating arthralgia during acute and chronic stages of the disease. RRV-induced cartilage degradation has been implicated as a cause of joint pain felt by RRV patients. Chondrocytes are a major cell type of cartilage and are involved in the production and maintenance of the cartilage matrix. It is thought that these cells may play a vital role in RRV disease pathogenesis. In this study, we used RNA-sequencing (RNA-Seq) to examine the transcriptomes of RRV-infected and bystander chondrocytes in the same environment. RRV containing green fluorescent protein (GFP) allowed for the separation of RRV-infected (GFP+) and bystander uninfected cells (GFP−). We found that whereas GFP+ and GFP− populations commonly presented similar gene expression profiles during infection, there were also unique signatures. For example, <i>RIMS2</i> and <i>FOXJ1</i> were unique to GFP+ cells, whilst <i>Aim2</i> and <i>CCL8</i> were only found in bystander chondrocytes. This indicates that careful selection of potential therapeutic targets is important to minimise adverse effects to the neighbouring uninfected cell populations. Our study serves as a resource to provide more information about the pathways and responses elicited by RRV in cells which are both infected and stimulated because of neighbouring infected cells. |
| first_indexed | 2024-03-09T11:02:28Z |
| format | Article |
| id | doaj.art-b337907797764b798854fd00c8d98a9d |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T11:02:28Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-b337907797764b798854fd00c8d98a9d2023-12-01T01:10:57ZengMDPI AGViruses1999-49152022-12-0115113610.3390/v15010136Pathways Activated by Infected and Bystander Chondrocytes in Response to Ross River Virus InfectionElisa X. Y. Lim0Julie A. Webster1Penny A. Rudd2Lara J. Herrero3Institute for Glycomics, Gold Coast Campus, Griffith University, Southport, QLD 4222, AustraliaInstitute for Glycomics, Gold Coast Campus, Griffith University, Southport, QLD 4222, AustraliaInstitute for Glycomics, Gold Coast Campus, Griffith University, Southport, QLD 4222, AustraliaInstitute for Glycomics, Gold Coast Campus, Griffith University, Southport, QLD 4222, AustraliaOld world alphaviruses, such as Ross River virus (RRV), cause debilitating arthralgia during acute and chronic stages of the disease. RRV-induced cartilage degradation has been implicated as a cause of joint pain felt by RRV patients. Chondrocytes are a major cell type of cartilage and are involved in the production and maintenance of the cartilage matrix. It is thought that these cells may play a vital role in RRV disease pathogenesis. In this study, we used RNA-sequencing (RNA-Seq) to examine the transcriptomes of RRV-infected and bystander chondrocytes in the same environment. RRV containing green fluorescent protein (GFP) allowed for the separation of RRV-infected (GFP+) and bystander uninfected cells (GFP−). We found that whereas GFP+ and GFP− populations commonly presented similar gene expression profiles during infection, there were also unique signatures. For example, <i>RIMS2</i> and <i>FOXJ1</i> were unique to GFP+ cells, whilst <i>Aim2</i> and <i>CCL8</i> were only found in bystander chondrocytes. This indicates that careful selection of potential therapeutic targets is important to minimise adverse effects to the neighbouring uninfected cell populations. Our study serves as a resource to provide more information about the pathways and responses elicited by RRV in cells which are both infected and stimulated because of neighbouring infected cells.https://www.mdpi.com/1999-4915/15/1/136cartilagealphavirusextracellular matrixarthritis |
| spellingShingle | Elisa X. Y. Lim Julie A. Webster Penny A. Rudd Lara J. Herrero Pathways Activated by Infected and Bystander Chondrocytes in Response to Ross River Virus Infection Viruses cartilage alphavirus extracellular matrix arthritis |
| title | Pathways Activated by Infected and Bystander Chondrocytes in Response to Ross River Virus Infection |
| title_full | Pathways Activated by Infected and Bystander Chondrocytes in Response to Ross River Virus Infection |
| title_fullStr | Pathways Activated by Infected and Bystander Chondrocytes in Response to Ross River Virus Infection |
| title_full_unstemmed | Pathways Activated by Infected and Bystander Chondrocytes in Response to Ross River Virus Infection |
| title_short | Pathways Activated by Infected and Bystander Chondrocytes in Response to Ross River Virus Infection |
| title_sort | pathways activated by infected and bystander chondrocytes in response to ross river virus infection |
| topic | cartilage alphavirus extracellular matrix arthritis |
| url | https://www.mdpi.com/1999-4915/15/1/136 |
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