The establishment of a chemically defined serum-free culture system for human dental pulp stem cells
Abstract Background The concept of establishing a dental stem cell (DSC) bank for oral and maxillofacial regeneration has become of great interest but it remains at a primitive stage. The routine application of serum-containing conditions for human DSC (hDSC) culture is in great controversy consider...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-07-01
|
| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13287-018-0928-8 |
| _version_ | 1818562047988727808 |
|---|---|
| author | Jingyi Xiao Dawei Yang Qiwen Li Weidong Tian Weihua Guo |
| author_facet | Jingyi Xiao Dawei Yang Qiwen Li Weidong Tian Weihua Guo |
| author_sort | Jingyi Xiao |
| collection | DOAJ |
| description | Abstract Background The concept of establishing a dental stem cell (DSC) bank for oral and maxillofacial regeneration has become of great interest but it remains at a primitive stage. The routine application of serum-containing conditions for human DSC (hDSC) culture is in great controversy considering that the animal-originated serum can cause serious ethical concerns and lead to increasingly irrelevant variables, errors, and poor repeatability of experiment results. Thus, this study aimed to establish a safe, stable and efficient hDSC serum-free culturing system for future DSC bank usage. Methods Dental pulp stem cells (DPSCs) from human permanent tooth pulp were isolated, expanded, passaged, and divided into two groups according to their culture conditions: group 1 was the serum-containing medium (SCM) group; and group 2 was the serum-free Essential 8 medium (E8) group. DPSCs were characterized first, followed by cell proliferation, pluripotency, and migration study in SCM and E8 medium. Results Human DPSCs (hDPSCs) in E8 medium demonstrated greater proliferation, pluripotency, migration ability and less apoptosis. hDPSCs could be successfully induced to the adipogenic, osteogenic, neurogenic, and chondrogenic lineages in E8 group. Real-time polymerase chain reaction indicated that the expression of PPAR-γ, RUNX2, OCN and MAP-2 was higher in E8 group. Conclusions Compared with serum-containing medium, E8 medium exhitibed higher ability in maintaining the cell proliferation, pluripotency, migration, and stability. This new serum-free culture environment might be applicable for hDSC culture in the future. |
| first_indexed | 2024-12-14T00:58:36Z |
| format | Article |
| id | doaj.art-b337dd978ba84766871bee4a17afda11 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-12-14T00:58:36Z |
| publishDate | 2018-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-b337dd978ba84766871bee4a17afda112022-12-21T23:23:25ZengBMCStem Cell Research & Therapy1757-65122018-07-019111510.1186/s13287-018-0928-8The establishment of a chemically defined serum-free culture system for human dental pulp stem cellsJingyi Xiao0Dawei Yang1Qiwen Li2Weidong Tian3Weihua Guo4State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & National EngineeringState Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & National EngineeringState Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & National EngineeringState Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & National EngineeringState Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & National EngineeringAbstract Background The concept of establishing a dental stem cell (DSC) bank for oral and maxillofacial regeneration has become of great interest but it remains at a primitive stage. The routine application of serum-containing conditions for human DSC (hDSC) culture is in great controversy considering that the animal-originated serum can cause serious ethical concerns and lead to increasingly irrelevant variables, errors, and poor repeatability of experiment results. Thus, this study aimed to establish a safe, stable and efficient hDSC serum-free culturing system for future DSC bank usage. Methods Dental pulp stem cells (DPSCs) from human permanent tooth pulp were isolated, expanded, passaged, and divided into two groups according to their culture conditions: group 1 was the serum-containing medium (SCM) group; and group 2 was the serum-free Essential 8 medium (E8) group. DPSCs were characterized first, followed by cell proliferation, pluripotency, and migration study in SCM and E8 medium. Results Human DPSCs (hDPSCs) in E8 medium demonstrated greater proliferation, pluripotency, migration ability and less apoptosis. hDPSCs could be successfully induced to the adipogenic, osteogenic, neurogenic, and chondrogenic lineages in E8 group. Real-time polymerase chain reaction indicated that the expression of PPAR-γ, RUNX2, OCN and MAP-2 was higher in E8 group. Conclusions Compared with serum-containing medium, E8 medium exhitibed higher ability in maintaining the cell proliferation, pluripotency, migration, and stability. This new serum-free culture environment might be applicable for hDSC culture in the future.http://link.springer.com/article/10.1186/s13287-018-0928-8Essential 8 MediumDental pulp stem cellsSerum-free cultureDental stem cell bank |
| spellingShingle | Jingyi Xiao Dawei Yang Qiwen Li Weidong Tian Weihua Guo The establishment of a chemically defined serum-free culture system for human dental pulp stem cells Stem Cell Research & Therapy Essential 8 Medium Dental pulp stem cells Serum-free culture Dental stem cell bank |
| title | The establishment of a chemically defined serum-free culture system for human dental pulp stem cells |
| title_full | The establishment of a chemically defined serum-free culture system for human dental pulp stem cells |
| title_fullStr | The establishment of a chemically defined serum-free culture system for human dental pulp stem cells |
| title_full_unstemmed | The establishment of a chemically defined serum-free culture system for human dental pulp stem cells |
| title_short | The establishment of a chemically defined serum-free culture system for human dental pulp stem cells |
| title_sort | establishment of a chemically defined serum free culture system for human dental pulp stem cells |
| topic | Essential 8 Medium Dental pulp stem cells Serum-free culture Dental stem cell bank |
| url | http://link.springer.com/article/10.1186/s13287-018-0928-8 |
| work_keys_str_mv | AT jingyixiao theestablishmentofachemicallydefinedserumfreeculturesystemforhumandentalpulpstemcells AT daweiyang theestablishmentofachemicallydefinedserumfreeculturesystemforhumandentalpulpstemcells AT qiwenli theestablishmentofachemicallydefinedserumfreeculturesystemforhumandentalpulpstemcells AT weidongtian theestablishmentofachemicallydefinedserumfreeculturesystemforhumandentalpulpstemcells AT weihuaguo theestablishmentofachemicallydefinedserumfreeculturesystemforhumandentalpulpstemcells AT jingyixiao establishmentofachemicallydefinedserumfreeculturesystemforhumandentalpulpstemcells AT daweiyang establishmentofachemicallydefinedserumfreeculturesystemforhumandentalpulpstemcells AT qiwenli establishmentofachemicallydefinedserumfreeculturesystemforhumandentalpulpstemcells AT weidongtian establishmentofachemicallydefinedserumfreeculturesystemforhumandentalpulpstemcells AT weihuaguo establishmentofachemicallydefinedserumfreeculturesystemforhumandentalpulpstemcells |