Facile Fabrication of 1-Methylimidazole/Cu Nanozyme with Enhanced Laccase Activity for Fast Degradation and Sensitive Detection of Phenol Compounds
Facile construction of functional nanomaterials with laccase-like activity is important in sustainable chemistry since laccase is featured as an efficient and promising catalyst especially for phenolic degradation but still has the challenges of high cost, low activity, poor stability and unsatisfie...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/15/4712 |
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| author | Yu Lei Bin He Shujun Huang Xinyan Chen Jian Sun |
| author_facet | Yu Lei Bin He Shujun Huang Xinyan Chen Jian Sun |
| author_sort | Yu Lei |
| collection | DOAJ |
| description | Facile construction of functional nanomaterials with laccase-like activity is important in sustainable chemistry since laccase is featured as an efficient and promising catalyst especially for phenolic degradation but still has the challenges of high cost, low activity, poor stability and unsatisfied recyclability. In this paper, we report a simple method to synthesize nanozymes with enhanced laccase-like activity by the self-assembly of copper ions with various imidazole derivatives. In the case of 1-methylimidazole as the ligand, the as-synthesized nanozyme (denoted as Cu-MIM) has the highest yield and best activity among the nanozymes prepared. Compared to laccase, the <i>K</i><sub>m</sub> of Cu-MIM nanozyme to phenol is much lower, and the <i>v</i><sub>max</sub> is 6.8 times higher. In addition, Cu-MIM maintains excellent stability in a variety of harsh environments, such as high pH, high temperature, high salt concentration, organic solvents and long-term storage. Based on the Cu-MIM nanozyme, we established a method for quantitatively detecting phenol concentration through a smartphone, which is believed to have important applications in environmental protection, pollutant detection and other fields. |
| first_indexed | 2024-03-09T10:06:47Z |
| format | Article |
| id | doaj.art-b338172ec7f74a31bf157898eb348814 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T10:06:47Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b338172ec7f74a31bf157898eb3488142023-12-01T23:02:47ZengMDPI AGMolecules1420-30492022-07-012715471210.3390/molecules27154712Facile Fabrication of 1-Methylimidazole/Cu Nanozyme with Enhanced Laccase Activity for Fast Degradation and Sensitive Detection of Phenol CompoundsYu Lei0Bin He1Shujun Huang2Xinyan Chen3Jian Sun4Key Laboratory of Molecular Medicine and Biotherapy in the Ministry of Industry and Information Technology, School of Life Science, Beijing Institute of Technology, Beijing 100081, ChinaKey Laboratory of Molecular Medicine and Biotherapy in the Ministry of Industry and Information Technology, School of Life Science, Beijing Institute of Technology, Beijing 100081, ChinaKey Laboratory of Molecular Medicine and Biotherapy in the Ministry of Industry and Information Technology, School of Life Science, Beijing Institute of Technology, Beijing 100081, ChinaKey Laboratory of Molecular Medicine and Biotherapy in the Ministry of Industry and Information Technology, School of Life Science, Beijing Institute of Technology, Beijing 100081, ChinaKey Laboratory of Molecular Medicine and Biotherapy in the Ministry of Industry and Information Technology, School of Life Science, Beijing Institute of Technology, Beijing 100081, ChinaFacile construction of functional nanomaterials with laccase-like activity is important in sustainable chemistry since laccase is featured as an efficient and promising catalyst especially for phenolic degradation but still has the challenges of high cost, low activity, poor stability and unsatisfied recyclability. In this paper, we report a simple method to synthesize nanozymes with enhanced laccase-like activity by the self-assembly of copper ions with various imidazole derivatives. In the case of 1-methylimidazole as the ligand, the as-synthesized nanozyme (denoted as Cu-MIM) has the highest yield and best activity among the nanozymes prepared. Compared to laccase, the <i>K</i><sub>m</sub> of Cu-MIM nanozyme to phenol is much lower, and the <i>v</i><sub>max</sub> is 6.8 times higher. In addition, Cu-MIM maintains excellent stability in a variety of harsh environments, such as high pH, high temperature, high salt concentration, organic solvents and long-term storage. Based on the Cu-MIM nanozyme, we established a method for quantitatively detecting phenol concentration through a smartphone, which is believed to have important applications in environmental protection, pollutant detection and other fields.https://www.mdpi.com/1420-3049/27/15/4712laccasephenolic oxidationnanozymeintelligent detectionself-assembly synthesis |
| spellingShingle | Yu Lei Bin He Shujun Huang Xinyan Chen Jian Sun Facile Fabrication of 1-Methylimidazole/Cu Nanozyme with Enhanced Laccase Activity for Fast Degradation and Sensitive Detection of Phenol Compounds Molecules laccase phenolic oxidation nanozyme intelligent detection self-assembly synthesis |
| title | Facile Fabrication of 1-Methylimidazole/Cu Nanozyme with Enhanced Laccase Activity for Fast Degradation and Sensitive Detection of Phenol Compounds |
| title_full | Facile Fabrication of 1-Methylimidazole/Cu Nanozyme with Enhanced Laccase Activity for Fast Degradation and Sensitive Detection of Phenol Compounds |
| title_fullStr | Facile Fabrication of 1-Methylimidazole/Cu Nanozyme with Enhanced Laccase Activity for Fast Degradation and Sensitive Detection of Phenol Compounds |
| title_full_unstemmed | Facile Fabrication of 1-Methylimidazole/Cu Nanozyme with Enhanced Laccase Activity for Fast Degradation and Sensitive Detection of Phenol Compounds |
| title_short | Facile Fabrication of 1-Methylimidazole/Cu Nanozyme with Enhanced Laccase Activity for Fast Degradation and Sensitive Detection of Phenol Compounds |
| title_sort | facile fabrication of 1 methylimidazole cu nanozyme with enhanced laccase activity for fast degradation and sensitive detection of phenol compounds |
| topic | laccase phenolic oxidation nanozyme intelligent detection self-assembly synthesis |
| url | https://www.mdpi.com/1420-3049/27/15/4712 |
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