Detection of a 4 bp Mutation in the 3′UTR Region of Goat <i>Sox9</i> Gene and Its Effect on the Growth Traits
The SRY-type HMG box 9 (<i>Sox9</i>) gene plays an important role in chondrocyte development as well as changes in hypertrophic chondrocytes, indicating that <i>Sox9</i> can regulate growth in animals. However, no studies to date have examined the correlation between variatio...
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MDPI AG
2020-04-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/4/672 |
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| author | Libang He Yi Bi Ruolan Wang Chuanying Pan Hong Chen Xianyong Lan Lei Qu |
| author_facet | Libang He Yi Bi Ruolan Wang Chuanying Pan Hong Chen Xianyong Lan Lei Qu |
| author_sort | Libang He |
| collection | DOAJ |
| description | The SRY-type HMG box 9 (<i>Sox9</i>) gene plays an important role in chondrocyte development as well as changes in hypertrophic chondrocytes, indicating that <i>Sox9</i> can regulate growth in animals. However, no studies to date have examined the correlation between variations in <i>Sox9</i> and growth traits in goats. Here, we found a 4 bp indel in the 3′UTR of <i>Sox9</i> and verified its association with growth traits in Shaanbei white cashmere goats (<i>n</i> = 1109). The frequencies of two genotypes (ID and II) were 0.397 and 0.603, respectively, and polymorphic information content (PIC) values showed that the indel had a medium PIC (PIC > 0.25). The 4 bp indel was significantly correlated with body length (<i>p</i> = 0.006), heart girth (<i>p</i> = 0.001), and hip width (<i>p</i> = 4.37 × 10 <sup>−4</sup>). Notably, individuals with the ID genotype had significantly superior phenotypic traits compared with individuals bearing the II genotype. Hence, we speculated that the 4 bp indel is an important mutation affecting growth traits in goat, and may serve as an effective DNA molecular marker for marker-assisted selection in goat breeding programs. |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T20:31:16Z |
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| spelling | doaj.art-b33843f243e9421fb5f381cbd66f64042023-11-19T21:26:33ZengMDPI AGAnimals2076-26152020-04-0110467210.3390/ani10040672Detection of a 4 bp Mutation in the 3′UTR Region of Goat <i>Sox9</i> Gene and Its Effect on the Growth TraitsLibang He0Yi Bi1Ruolan Wang2Chuanying Pan3Hong Chen4Xianyong Lan5Lei Qu6College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, ChinaShaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin 719000, Shaanxi, ChinaThe SRY-type HMG box 9 (<i>Sox9</i>) gene plays an important role in chondrocyte development as well as changes in hypertrophic chondrocytes, indicating that <i>Sox9</i> can regulate growth in animals. However, no studies to date have examined the correlation between variations in <i>Sox9</i> and growth traits in goats. Here, we found a 4 bp indel in the 3′UTR of <i>Sox9</i> and verified its association with growth traits in Shaanbei white cashmere goats (<i>n</i> = 1109). The frequencies of two genotypes (ID and II) were 0.397 and 0.603, respectively, and polymorphic information content (PIC) values showed that the indel had a medium PIC (PIC > 0.25). The 4 bp indel was significantly correlated with body length (<i>p</i> = 0.006), heart girth (<i>p</i> = 0.001), and hip width (<i>p</i> = 4.37 × 10 <sup>−4</sup>). Notably, individuals with the ID genotype had significantly superior phenotypic traits compared with individuals bearing the II genotype. Hence, we speculated that the 4 bp indel is an important mutation affecting growth traits in goat, and may serve as an effective DNA molecular marker for marker-assisted selection in goat breeding programs.https://www.mdpi.com/2076-2615/10/4/672growth performancechondrocyte<i>Sox9</i>polymorphismgoat |
| spellingShingle | Libang He Yi Bi Ruolan Wang Chuanying Pan Hong Chen Xianyong Lan Lei Qu Detection of a 4 bp Mutation in the 3′UTR Region of Goat <i>Sox9</i> Gene and Its Effect on the Growth Traits Animals growth performance chondrocyte <i>Sox9</i> polymorphism goat |
| title | Detection of a 4 bp Mutation in the 3′UTR Region of Goat <i>Sox9</i> Gene and Its Effect on the Growth Traits |
| title_full | Detection of a 4 bp Mutation in the 3′UTR Region of Goat <i>Sox9</i> Gene and Its Effect on the Growth Traits |
| title_fullStr | Detection of a 4 bp Mutation in the 3′UTR Region of Goat <i>Sox9</i> Gene and Its Effect on the Growth Traits |
| title_full_unstemmed | Detection of a 4 bp Mutation in the 3′UTR Region of Goat <i>Sox9</i> Gene and Its Effect on the Growth Traits |
| title_short | Detection of a 4 bp Mutation in the 3′UTR Region of Goat <i>Sox9</i> Gene and Its Effect on the Growth Traits |
| title_sort | detection of a 4 bp mutation in the 3 utr region of goat i sox9 i gene and its effect on the growth traits |
| topic | growth performance chondrocyte <i>Sox9</i> polymorphism goat |
| url | https://www.mdpi.com/2076-2615/10/4/672 |
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