Transcriptomic and metabolic studies on the role of inorganic and organic iodine compounds in lettuce plants
Abstract Iodine (I) is considered a beneficial element or even micronutrient for plants. The aim of this study was to determine the molecular and physiological processes of uptake, transport, and metabolism of I applied to lettuce plants. KIO3, KIO3 + salicylic acid, 5-iodosalicylic acid and 3,5-dii...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-34873-7 |
| _version_ | 1797818086284328960 |
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| author | Sylwester Smoleń Małgorzata Czernicka Kinga Kęska-Izworska Iwona Kowalska Dariusz Grzebelus Joanna Pitala Mariya Halka Łukasz Skoczylas Małgorzata Tabaszewska Marta Liszka-Skoczylas Marlena Grzanka Iwona Ledwożyw-Smoleń Aneta Koronowicz Joanna Krzemińska Olga Sularz Daniel Kiełbasa Jakub Neupauer Peter Kováčik |
| author_facet | Sylwester Smoleń Małgorzata Czernicka Kinga Kęska-Izworska Iwona Kowalska Dariusz Grzebelus Joanna Pitala Mariya Halka Łukasz Skoczylas Małgorzata Tabaszewska Marta Liszka-Skoczylas Marlena Grzanka Iwona Ledwożyw-Smoleń Aneta Koronowicz Joanna Krzemińska Olga Sularz Daniel Kiełbasa Jakub Neupauer Peter Kováčik |
| author_sort | Sylwester Smoleń |
| collection | DOAJ |
| description | Abstract Iodine (I) is considered a beneficial element or even micronutrient for plants. The aim of this study was to determine the molecular and physiological processes of uptake, transport, and metabolism of I applied to lettuce plants. KIO3, KIO3 + salicylic acid, 5-iodosalicylic acid and 3,5-diiodosalicylic acid were applied. RNA-sequencing was executed using 18 cDNA libraries constructed separately for leaves and roots from KIO3, SA and control plants. De novo transcriptome assembly generated 1937.76 million sequence reads resulting in 27,163 transcripts with N50 of 1638 bp. 329 differentially expressed genes (DEGs) in roots were detected after application of KIO3, out of which 252 genes were up-regulated, and 77 were down-regulated. In leaves, 9 genes revealed differential expression pattern. DEGs analysis indicated its involvement in such metabolic pathways and processes as: chloride transmembrane transport, phenylpropanoid metabolism, positive regulation of defense response and leaf abscission, and also ubiquinone and other terpenoid-quinone biosynthesis, protein processing in endoplasmic reticulum, circadian rhythm including flowering induction as well as a putative PDTHA (i.e. Plant Derived Thyroid Hormone Analogs) metabolic pathway. qRT-PCR of selected genes suggested their participation in the transport and metabolism of iodine compounds, biosynthesis of primary and secondary metabolites, PDTHA pathway and flowering induction. |
| first_indexed | 2024-03-13T09:02:57Z |
| format | Article |
| id | doaj.art-b33a2935541a45379c9b048ba51ceca9 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T09:02:57Z |
| publishDate | 2023-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b33a2935541a45379c9b048ba51ceca92023-05-28T11:13:25ZengNature PortfolioScientific Reports2045-23222023-05-0113111410.1038/s41598-023-34873-7Transcriptomic and metabolic studies on the role of inorganic and organic iodine compounds in lettuce plantsSylwester Smoleń0Małgorzata Czernicka1Kinga Kęska-Izworska2Iwona Kowalska3Dariusz Grzebelus4Joanna Pitala5Mariya Halka6Łukasz Skoczylas7Małgorzata Tabaszewska8Marta Liszka-Skoczylas9Marlena Grzanka10Iwona Ledwożyw-Smoleń11Aneta Koronowicz12Joanna Krzemińska13Olga Sularz14Daniel Kiełbasa15Jakub Neupauer16Peter Kováčik17Department of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in KrakowDepartment of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in KrakowDepartment of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in KrakowDepartment of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in KrakowDepartment of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in KrakowLaboratory of Mass Spectrometry, Faculty of Biotechnology and Horticulture, University of Agriculture in KrakowDepartment of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in KrakowDepartment of Plant Product Technology and Nutrition Hygiene, Faculty of Food Technology, University of Agriculture in KrakowDepartment of Plant Product Technology and Nutrition Hygiene, Faculty of Food Technology, University of Agriculture in KrakowDepartment of Engineering and Machinery for Food Industry, Faculty of Food Technology, University of Agriculture in KrakowDepartment of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in KrakowDepartment of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in KrakowDepartment of Human Nutrition and Dietetics, Faculty of Food Technology, University of Agriculture in KrakowDepartment of Human Nutrition and Dietetics, Faculty of Food Technology, University of Agriculture in KrakowDepartment of Human Nutrition and Dietetics, Faculty of Food Technology, University of Agriculture in KrakowDepartment of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in KrakowDepartment of Agrochemistry and Plant Nutrition, Slovak University of Agriculture in NitraDepartment of Agrochemistry and Plant Nutrition, Slovak University of Agriculture in NitraAbstract Iodine (I) is considered a beneficial element or even micronutrient for plants. The aim of this study was to determine the molecular and physiological processes of uptake, transport, and metabolism of I applied to lettuce plants. KIO3, KIO3 + salicylic acid, 5-iodosalicylic acid and 3,5-diiodosalicylic acid were applied. RNA-sequencing was executed using 18 cDNA libraries constructed separately for leaves and roots from KIO3, SA and control plants. De novo transcriptome assembly generated 1937.76 million sequence reads resulting in 27,163 transcripts with N50 of 1638 bp. 329 differentially expressed genes (DEGs) in roots were detected after application of KIO3, out of which 252 genes were up-regulated, and 77 were down-regulated. In leaves, 9 genes revealed differential expression pattern. DEGs analysis indicated its involvement in such metabolic pathways and processes as: chloride transmembrane transport, phenylpropanoid metabolism, positive regulation of defense response and leaf abscission, and also ubiquinone and other terpenoid-quinone biosynthesis, protein processing in endoplasmic reticulum, circadian rhythm including flowering induction as well as a putative PDTHA (i.e. Plant Derived Thyroid Hormone Analogs) metabolic pathway. qRT-PCR of selected genes suggested their participation in the transport and metabolism of iodine compounds, biosynthesis of primary and secondary metabolites, PDTHA pathway and flowering induction.https://doi.org/10.1038/s41598-023-34873-7 |
| spellingShingle | Sylwester Smoleń Małgorzata Czernicka Kinga Kęska-Izworska Iwona Kowalska Dariusz Grzebelus Joanna Pitala Mariya Halka Łukasz Skoczylas Małgorzata Tabaszewska Marta Liszka-Skoczylas Marlena Grzanka Iwona Ledwożyw-Smoleń Aneta Koronowicz Joanna Krzemińska Olga Sularz Daniel Kiełbasa Jakub Neupauer Peter Kováčik Transcriptomic and metabolic studies on the role of inorganic and organic iodine compounds in lettuce plants Scientific Reports |
| title | Transcriptomic and metabolic studies on the role of inorganic and organic iodine compounds in lettuce plants |
| title_full | Transcriptomic and metabolic studies on the role of inorganic and organic iodine compounds in lettuce plants |
| title_fullStr | Transcriptomic and metabolic studies on the role of inorganic and organic iodine compounds in lettuce plants |
| title_full_unstemmed | Transcriptomic and metabolic studies on the role of inorganic and organic iodine compounds in lettuce plants |
| title_short | Transcriptomic and metabolic studies on the role of inorganic and organic iodine compounds in lettuce plants |
| title_sort | transcriptomic and metabolic studies on the role of inorganic and organic iodine compounds in lettuce plants |
| url | https://doi.org/10.1038/s41598-023-34873-7 |
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