Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm
Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis in small ruminants, a chronic disease characterized by the development of granulomas in superficial and visceral lymph nodes as well as in several organs. An important characteristic of the infection with this bacte...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2021.700030/full |
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| author | Laerte Marlon Santos Laerte Marlon Santos Daniela Méria Rodrigues Maurício Alcantara Kalil Vasco Azevedo Roberto Meyer Marcelo Andres Umsza-Guez Bruna Aparecida Machado Nubia Seyffert Ricardo Wagner Portela |
| author_facet | Laerte Marlon Santos Laerte Marlon Santos Daniela Méria Rodrigues Maurício Alcantara Kalil Vasco Azevedo Roberto Meyer Marcelo Andres Umsza-Guez Bruna Aparecida Machado Nubia Seyffert Ricardo Wagner Portela |
| author_sort | Laerte Marlon Santos |
| collection | DOAJ |
| description | Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis in small ruminants, a chronic disease characterized by the development of granulomas in superficial and visceral lymph nodes as well as in several organs. An important characteristic of the infection with this bacterium is the formation of a biofilm and the absence of effective antibiotic therapy against the disease. From this scenario, the objective of this study was to evaluate the susceptibility of C. pseudotuberculosis to conventional antibiotics and to red, green, and brown propolis extracts obtained by the supercritical and ethanolic extraction methods as well as its activity in the bacterial biofilm. The results of the sensitivity test using antibiotics indicated a sensitivity of C. pseudotuberculosis strains to the antimicrobial agents. The ethanolic extract of green propolis and the supercritical red propolis extract showed the best antibacterial activities against planktonic C. pseudotuberculosis. A lower antimicrobial activity of the brown propolis extract was identified. Propolis extracts were effective in interfering with the formation of the C. pseudotuberculosis biofilm but had little activity on the consolidated biofilm. In conclusion, propolis extracts are more effective against C. pseudotuberculosis in the planktonic stage, being able to interfere with the formation of bacterial biofilm. However, the action of propolis extracts in a sessile and structured microbial biofilm is reduced. |
| first_indexed | 2024-12-22T13:49:55Z |
| format | Article |
| id | doaj.art-b33ca65b3c904859965717ecc8647303 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-22T13:49:55Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-b33ca65b3c904859965717ecc86473032022-12-21T18:23:41ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-09-01810.3389/fvets.2021.700030700030Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated BiofilmLaerte Marlon Santos0Laerte Marlon Santos1Daniela Méria Rodrigues2Maurício Alcantara Kalil3Vasco Azevedo4Roberto Meyer5Marcelo Andres Umsza-Guez6Bruna Aparecida Machado7Nubia Seyffert8Ricardo Wagner Portela9Laboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BrazilInstituto de Tecnologia em Saúde, CIMATEC-SENAI, Salvador, BrazilLaboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BrazilLaboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BrazilLaboratório de Genética Celular e Molecular, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, BrazilLaboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BrazilInstituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BrazilInstituto de Tecnologia em Saúde, CIMATEC-SENAI, Salvador, BrazilLaboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BrazilLaboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BrazilCorynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis in small ruminants, a chronic disease characterized by the development of granulomas in superficial and visceral lymph nodes as well as in several organs. An important characteristic of the infection with this bacterium is the formation of a biofilm and the absence of effective antibiotic therapy against the disease. From this scenario, the objective of this study was to evaluate the susceptibility of C. pseudotuberculosis to conventional antibiotics and to red, green, and brown propolis extracts obtained by the supercritical and ethanolic extraction methods as well as its activity in the bacterial biofilm. The results of the sensitivity test using antibiotics indicated a sensitivity of C. pseudotuberculosis strains to the antimicrobial agents. The ethanolic extract of green propolis and the supercritical red propolis extract showed the best antibacterial activities against planktonic C. pseudotuberculosis. A lower antimicrobial activity of the brown propolis extract was identified. Propolis extracts were effective in interfering with the formation of the C. pseudotuberculosis biofilm but had little activity on the consolidated biofilm. In conclusion, propolis extracts are more effective against C. pseudotuberculosis in the planktonic stage, being able to interfere with the formation of bacterial biofilm. However, the action of propolis extracts in a sessile and structured microbial biofilm is reduced.https://www.frontiersin.org/articles/10.3389/fvets.2021.700030/fullcaseous lymphadenitissmall ruminantsmicrobial resistancenatural extractssupercritical extraction |
| spellingShingle | Laerte Marlon Santos Laerte Marlon Santos Daniela Méria Rodrigues Maurício Alcantara Kalil Vasco Azevedo Roberto Meyer Marcelo Andres Umsza-Guez Bruna Aparecida Machado Nubia Seyffert Ricardo Wagner Portela Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm Frontiers in Veterinary Science caseous lymphadenitis small ruminants microbial resistance natural extracts supercritical extraction |
| title | Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm |
| title_full | Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm |
| title_fullStr | Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm |
| title_full_unstemmed | Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm |
| title_short | Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm |
| title_sort | activity of ethanolic and supercritical propolis extracts in corynebacterium pseudotuberculosis and its associated biofilm |
| topic | caseous lymphadenitis small ruminants microbial resistance natural extracts supercritical extraction |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2021.700030/full |
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