Comparison of Composition, Free-Radical-Scavenging Capacity, and Antibiosis of Fresh and Dry Leave Aqueous Extract from <i>Michelia shiluensis</i>
Numerous plants of medicinal value grow on Hainan Island (China). Given the lack of knowledge on the phytochemical and pharmacological properties of <i>Michelia shiluensis</i> Chun and Y. F. Wu (<i>M. shiluensis</i>), the application of natural antioxidants and antimicrobials...
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MDPI AG
2023-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/16/5935 |
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| author | Wentao Wu Gaoyu Li Weijuan Zhou Enbo Wang Xia Zhao Xiqiang Song Ying Zhao |
| author_facet | Wentao Wu Gaoyu Li Weijuan Zhou Enbo Wang Xia Zhao Xiqiang Song Ying Zhao |
| author_sort | Wentao Wu |
| collection | DOAJ |
| description | Numerous plants of medicinal value grow on Hainan Island (China). Given the lack of knowledge on the phytochemical and pharmacological properties of <i>Michelia shiluensis</i> Chun and Y. F. Wu (<i>M. shiluensis</i>), the application of natural antioxidants and antimicrobials in the food industry has attracted increasing interest. This study aimed to compare the chemical composition, free-radical-scavenging capacity, and antibiosis of aqueous extracts of the fresh and dried leaves of <i>M. shiluensis.</i> The aqueous extract of the leaves of <i>M. shiluensis</i> was obtained using steam distillation, and its chemical components were separated and identified via gas chromatography–mass spectrometry (GC-MS). The free-radical-scavenging capacity and antibiosis were determined. Further, 28 and 20 compounds were isolated from the fresh leaf aqueous extract of <i>M. shiluensis</i> (MSFLAE) and dried leaf aqueous extract of <i>M. shiluensis</i> (MSDLAE), respectively. The free-radical-scavenging capacity of MSFLAE and MSDLAE was determined by the 2,2-diphenyl-1 picrylhydrazyl (DPPH) method, which was 43.43% and 38.74%, respectively. The scavenging capacity of MSFLAE and MSDLAE determined by the 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonate (ABTS)) method was 46.90% and 25.99%, respectively. The iron ion reduction capacity of MSFLAE and MSDLAE was determined by the ferric-reducing antioxidant power (FRAP) method as 94.7 and 62.9 μmol Fe<sup>2</sup>⁺/L, respectively. This indicated that the two leaf aqueous extracts had a certain free-radical-scavenging capacity, and the capacity of MSFLAE was higher than that of MSDLAE. The antibiosis of the two leaf aqueous extracts on the three foodborne pathogenic bacteria was low, but the antimicrobial effects on Gram-positive bacteria were better than those on Gram-negative bacteria. The antibiosis of MSFLAE on <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> was greater than that of MSDLAE. Finally, MSFLAE and MSDLAE both had certain free-radical-scavenging capacities and antibiosis, confirming that the use of this plant in the research and development of natural antioxidants and antibacterial agents was reasonable. Plant aqueous extracts are an essential source of related phytochemistry and have immense pharmacological potential. |
| first_indexed | 2024-03-10T23:42:57Z |
| format | Article |
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| language | English |
| last_indexed | 2024-03-10T23:42:57Z |
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| spelling | doaj.art-b33ca7ac3854473183a5da0e43c501f32023-11-19T02:22:04ZengMDPI AGMolecules1420-30492023-08-012816593510.3390/molecules28165935Comparison of Composition, Free-Radical-Scavenging Capacity, and Antibiosis of Fresh and Dry Leave Aqueous Extract from <i>Michelia shiluensis</i>Wentao Wu0Gaoyu Li1Weijuan Zhou2Enbo Wang3Xia Zhao4Xiqiang Song5Ying Zhao6Hainan Key Laboratory of Biology of Tropical Flowers and Trees Resources, Forestry Institute, Hainan University, Haikou 570228, ChinaHainan Key Laboratory of Biology of Tropical Flowers and Trees Resources, Forestry Institute, Hainan University, Haikou 570228, ChinaHainan Key Laboratory of Biology of Tropical Flowers and Trees Resources, Forestry Institute, Hainan University, Haikou 570228, ChinaHainan Key Laboratory of Biology of Tropical Flowers and Trees Resources, Forestry Institute, Hainan University, Haikou 570228, ChinaHainan Key Laboratory of Biology of Tropical Flowers and Trees Resources, Forestry Institute, Hainan University, Haikou 570228, ChinaHainan Key Laboratory of Biology of Tropical Flowers and Trees Resources, Forestry Institute, Hainan University, Haikou 570228, ChinaHainan Key Laboratory of Biology of Tropical Flowers and Trees Resources, Forestry Institute, Hainan University, Haikou 570228, ChinaNumerous plants of medicinal value grow on Hainan Island (China). Given the lack of knowledge on the phytochemical and pharmacological properties of <i>Michelia shiluensis</i> Chun and Y. F. Wu (<i>M. shiluensis</i>), the application of natural antioxidants and antimicrobials in the food industry has attracted increasing interest. This study aimed to compare the chemical composition, free-radical-scavenging capacity, and antibiosis of aqueous extracts of the fresh and dried leaves of <i>M. shiluensis.</i> The aqueous extract of the leaves of <i>M. shiluensis</i> was obtained using steam distillation, and its chemical components were separated and identified via gas chromatography–mass spectrometry (GC-MS). The free-radical-scavenging capacity and antibiosis were determined. Further, 28 and 20 compounds were isolated from the fresh leaf aqueous extract of <i>M. shiluensis</i> (MSFLAE) and dried leaf aqueous extract of <i>M. shiluensis</i> (MSDLAE), respectively. The free-radical-scavenging capacity of MSFLAE and MSDLAE was determined by the 2,2-diphenyl-1 picrylhydrazyl (DPPH) method, which was 43.43% and 38.74%, respectively. The scavenging capacity of MSFLAE and MSDLAE determined by the 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonate (ABTS)) method was 46.90% and 25.99%, respectively. The iron ion reduction capacity of MSFLAE and MSDLAE was determined by the ferric-reducing antioxidant power (FRAP) method as 94.7 and 62.9 μmol Fe<sup>2</sup>⁺/L, respectively. This indicated that the two leaf aqueous extracts had a certain free-radical-scavenging capacity, and the capacity of MSFLAE was higher than that of MSDLAE. The antibiosis of the two leaf aqueous extracts on the three foodborne pathogenic bacteria was low, but the antimicrobial effects on Gram-positive bacteria were better than those on Gram-negative bacteria. The antibiosis of MSFLAE on <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> was greater than that of MSDLAE. Finally, MSFLAE and MSDLAE both had certain free-radical-scavenging capacities and antibiosis, confirming that the use of this plant in the research and development of natural antioxidants and antibacterial agents was reasonable. Plant aqueous extracts are an essential source of related phytochemistry and have immense pharmacological potential.https://www.mdpi.com/1420-3049/28/16/5935<i>Michelia shiluensis</i>antibiosisfree-radical-scavenging capacitygas chromatography–mass spectrometry |
| spellingShingle | Wentao Wu Gaoyu Li Weijuan Zhou Enbo Wang Xia Zhao Xiqiang Song Ying Zhao Comparison of Composition, Free-Radical-Scavenging Capacity, and Antibiosis of Fresh and Dry Leave Aqueous Extract from <i>Michelia shiluensis</i> Molecules <i>Michelia shiluensis</i> antibiosis free-radical-scavenging capacity gas chromatography–mass spectrometry |
| title | Comparison of Composition, Free-Radical-Scavenging Capacity, and Antibiosis of Fresh and Dry Leave Aqueous Extract from <i>Michelia shiluensis</i> |
| title_full | Comparison of Composition, Free-Radical-Scavenging Capacity, and Antibiosis of Fresh and Dry Leave Aqueous Extract from <i>Michelia shiluensis</i> |
| title_fullStr | Comparison of Composition, Free-Radical-Scavenging Capacity, and Antibiosis of Fresh and Dry Leave Aqueous Extract from <i>Michelia shiluensis</i> |
| title_full_unstemmed | Comparison of Composition, Free-Radical-Scavenging Capacity, and Antibiosis of Fresh and Dry Leave Aqueous Extract from <i>Michelia shiluensis</i> |
| title_short | Comparison of Composition, Free-Radical-Scavenging Capacity, and Antibiosis of Fresh and Dry Leave Aqueous Extract from <i>Michelia shiluensis</i> |
| title_sort | comparison of composition free radical scavenging capacity and antibiosis of fresh and dry leave aqueous extract from i michelia shiluensis i |
| topic | <i>Michelia shiluensis</i> antibiosis free-radical-scavenging capacity gas chromatography–mass spectrometry |
| url | https://www.mdpi.com/1420-3049/28/16/5935 |
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