Antibiotic resistance profiles and activity of clove essential oil (Syzygium aromaticum) against Pseudomonas aeruginosa isolated of canine otitis
Background and Aim: Pseudomonas aeruginosa is often isolated from acute and chronic otitis and deep pyoderma in dogs. The increase in bacterial resistance to antibiotics induced the need for alternative therapies to treat infections, with an emphasis on essential oils (EOs). This study aimed to inve...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Veterinary World
2022-10-01
|
| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.15/October-2022/16.pdf |
| _version_ | 1811257163757125632 |
|---|---|
| author | Larissa Vieira Costa Janaina Marcela Assunção Rosa Moreira Isabela de Godoy Menezes Valéria Dutra Arleana do Bom Parto Ferreira de Almeida |
| author_facet | Larissa Vieira Costa Janaina Marcela Assunção Rosa Moreira Isabela de Godoy Menezes Valéria Dutra Arleana do Bom Parto Ferreira de Almeida |
| author_sort | Larissa Vieira Costa |
| collection | DOAJ |
| description | Background and Aim: Pseudomonas aeruginosa is often isolated from acute and chronic otitis and deep pyoderma in dogs. The increase in bacterial resistance to antibiotics induced the need for alternative therapies to treat infections, with an emphasis on essential oils (EOs). This study aimed to investigate clove oil's in vitro bactericidal action as a therapeutic alternative against strains of P. aeruginosa isolated from canine otitis.
Materials and Methods: The antibacterial activity of clove oil was evaluated by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using the broth microdilution technique in 96-well plates. Serial concentrations of 10–0.31% of the oil were used, equivalent to 104.5–3.26 mg/mL. The susceptibility of isolates to different classes of antibiotics was determined by the disk diffusion technique using 20 antibiotics belonging to eight classes. Isolates resistant to at least one antibiotic of three different classes were considered multidrug-resistant (MDR).
Results: A high occurrence of resistance was observed for three antibiotics belonging to the cephalosporin classes (cefadroxil, cephalexin, and ceftriaxone), namely, sulfamethoxazole + trimethoprime, doxycycline, and enrofloxacin. The lowest resistance rates were observed for meropenem (4.88%), amikacin (12.20%), and tobramycin (12.2%). All isolates were susceptible to clove oil with an equivalent MIC and MBC from 3.26 to 6.53 mg/mL. Eugenol was the major component of the oil.
Conclusion: Clove EO was effective against MDR strains of P. aeruginosa, indicating an alternative for developing an efficient and low-cost antimicrobial agent to treat canine otitis. |
| first_indexed | 2024-04-12T17:53:02Z |
| format | Article |
| id | doaj.art-b33d0075737f4539ad94093dc2861b46 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-04-12T17:53:02Z |
| publishDate | 2022-10-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-b33d0075737f4539ad94093dc2861b462022-12-22T03:22:27ZengVeterinary WorldVeterinary World0972-89882231-09162022-10-0115102499250510.14202/vetworld.2022.2499-2505Antibiotic resistance profiles and activity of clove essential oil (Syzygium aromaticum) against Pseudomonas aeruginosa isolated of canine otitisLarissa Vieira Costa0https://orcid.org/0000-0002-1693-8146Janaina Marcela Assunção Rosa Moreira1https://orcid.org/0000-0001-5397-6064Isabela de Godoy Menezes2https://orcid.org/0000-0003-2577-8719Valéria Dutra3https://orcid.org/0000-0002-6630-2293Arleana do Bom Parto Ferreira de Almeida4https://orcid.org/0000-0002-6694-1407Program of Postgraduate in Veterinary Sciences, Faculty of Veterinary Medicine, Federal University of Mato Grosso, Cuiabá – Mato Grosso, Brazil.Program of Postgraduate in Veterinary Sciences, Faculty of Veterinary Medicine, Federal University of Mato Grosso, Cuiabá – Mato Grosso, Brazil.Medical Sciences College, Federal University of São Paulo, São Paulo, Brazil.Veterinary Hospital, Faculty of Veterinary Medicine, Federal University of Mato Grosso, Cuiabá – Mato Grosso, Brazil.Veterinary Hospital, Faculty of Veterinary Medicine, Federal University of Mato Grosso, Cuiabá – Mato Grosso, Brazil.Background and Aim: Pseudomonas aeruginosa is often isolated from acute and chronic otitis and deep pyoderma in dogs. The increase in bacterial resistance to antibiotics induced the need for alternative therapies to treat infections, with an emphasis on essential oils (EOs). This study aimed to investigate clove oil's in vitro bactericidal action as a therapeutic alternative against strains of P. aeruginosa isolated from canine otitis. Materials and Methods: The antibacterial activity of clove oil was evaluated by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using the broth microdilution technique in 96-well plates. Serial concentrations of 10–0.31% of the oil were used, equivalent to 104.5–3.26 mg/mL. The susceptibility of isolates to different classes of antibiotics was determined by the disk diffusion technique using 20 antibiotics belonging to eight classes. Isolates resistant to at least one antibiotic of three different classes were considered multidrug-resistant (MDR). Results: A high occurrence of resistance was observed for three antibiotics belonging to the cephalosporin classes (cefadroxil, cephalexin, and ceftriaxone), namely, sulfamethoxazole + trimethoprime, doxycycline, and enrofloxacin. The lowest resistance rates were observed for meropenem (4.88%), amikacin (12.20%), and tobramycin (12.2%). All isolates were susceptible to clove oil with an equivalent MIC and MBC from 3.26 to 6.53 mg/mL. Eugenol was the major component of the oil. Conclusion: Clove EO was effective against MDR strains of P. aeruginosa, indicating an alternative for developing an efficient and low-cost antimicrobial agent to treat canine otitis.http://www.veterinaryworld.org/Vol.15/October-2022/16.pdfessential oilmultidrug resistancepseudomonas aeruginosasusceptibility |
| spellingShingle | Larissa Vieira Costa Janaina Marcela Assunção Rosa Moreira Isabela de Godoy Menezes Valéria Dutra Arleana do Bom Parto Ferreira de Almeida Antibiotic resistance profiles and activity of clove essential oil (Syzygium aromaticum) against Pseudomonas aeruginosa isolated of canine otitis Veterinary World essential oil multidrug resistance pseudomonas aeruginosa susceptibility |
| title | Antibiotic resistance profiles and activity of clove essential oil (Syzygium aromaticum) against Pseudomonas aeruginosa isolated of canine otitis |
| title_full | Antibiotic resistance profiles and activity of clove essential oil (Syzygium aromaticum) against Pseudomonas aeruginosa isolated of canine otitis |
| title_fullStr | Antibiotic resistance profiles and activity of clove essential oil (Syzygium aromaticum) against Pseudomonas aeruginosa isolated of canine otitis |
| title_full_unstemmed | Antibiotic resistance profiles and activity of clove essential oil (Syzygium aromaticum) against Pseudomonas aeruginosa isolated of canine otitis |
| title_short | Antibiotic resistance profiles and activity of clove essential oil (Syzygium aromaticum) against Pseudomonas aeruginosa isolated of canine otitis |
| title_sort | antibiotic resistance profiles and activity of clove essential oil syzygium aromaticum against pseudomonas aeruginosa isolated of canine otitis |
| topic | essential oil multidrug resistance pseudomonas aeruginosa susceptibility |
| url | http://www.veterinaryworld.org/Vol.15/October-2022/16.pdf |
| work_keys_str_mv | AT larissavieiracosta antibioticresistanceprofilesandactivityofcloveessentialoilsyzygiumaromaticumagainstpseudomonasaeruginosaisolatedofcanineotitis AT janainamarcelaassuncaorosamoreira antibioticresistanceprofilesandactivityofcloveessentialoilsyzygiumaromaticumagainstpseudomonasaeruginosaisolatedofcanineotitis AT isabeladegodoymenezes antibioticresistanceprofilesandactivityofcloveessentialoilsyzygiumaromaticumagainstpseudomonasaeruginosaisolatedofcanineotitis AT valeriadutra antibioticresistanceprofilesandactivityofcloveessentialoilsyzygiumaromaticumagainstpseudomonasaeruginosaisolatedofcanineotitis AT arleanadobompartoferreiradealmeida antibioticresistanceprofilesandactivityofcloveessentialoilsyzygiumaromaticumagainstpseudomonasaeruginosaisolatedofcanineotitis |