Neuroprotective Effects of Cryptotanshinone in a Direct Reprogramming Model of Parkinson’s Disease
Parkinson’s disease (PD) is a well-known age-related neurodegenerative disease. Considering the vital importance of disease modeling based on reprogramming technology, we adopted direct reprogramming to human-induced neuronal progenitor cells (hiNPCs) for in vitro assessment of potential therapeutic...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/16/3602 |
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| author | Joo-Eun Lee Hyuna Sim Hee Min Yoo Minhyung Lee Aruem Baek Young-Joo Jeon Kang-Sik Seo Mi-Young Son Joo Seog Yoon Janghwan Kim |
| author_facet | Joo-Eun Lee Hyuna Sim Hee Min Yoo Minhyung Lee Aruem Baek Young-Joo Jeon Kang-Sik Seo Mi-Young Son Joo Seog Yoon Janghwan Kim |
| author_sort | Joo-Eun Lee |
| collection | DOAJ |
| description | Parkinson’s disease (PD) is a well-known age-related neurodegenerative disease. Considering the vital importance of disease modeling based on reprogramming technology, we adopted direct reprogramming to human-induced neuronal progenitor cells (hiNPCs) for in vitro assessment of potential therapeutics. In this study, we investigated the neuroprotective effects of cryptotanshinone (CTN), which has been reported to have antioxidant properties, through PD patient-derived hiNPCs (PD-iNPCs) model with induced oxidative stress and cell death by the proteasome inhibitor MG132. A cytotoxicity assay showed that CTN possesses anti-apoptotic properties in PD-hiNPCs. CTN treatment significantly reduced cellular apoptosis through mitochondrial restoration, such as the reduction in mitochondrial reactive oxygen species and increments of mitochondrial membrane potential. These effects of CTN are mediated via the nuclear factor erythroid 2-related factor 2 (NRF2) pathway in PD-hiNPCs. Consequently, CTN could be a potential antioxidant reagent for preventing disease-related pathological phenotypes of PD. |
| first_indexed | 2024-03-10T17:48:11Z |
| format | Article |
| id | doaj.art-b33d6504793c43b1a52339a90eeb1433 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T17:48:11Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b33d6504793c43b1a52339a90eeb14332023-11-20T09:28:13ZengMDPI AGMolecules1420-30492020-08-012516360210.3390/molecules25163602Neuroprotective Effects of Cryptotanshinone in a Direct Reprogramming Model of Parkinson’s DiseaseJoo-Eun Lee0Hyuna Sim1Hee Min Yoo2Minhyung Lee3Aruem Baek4Young-Joo Jeon5Kang-Sik Seo6Mi-Young Son7Joo Seog Yoon8Janghwan Kim9Stem Cell Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, KoreaStem Cell Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, KoreaGroup for Biometrology, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, KoreaStem Cell Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, KoreaStem Cell Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, KoreaStem Cell Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, KoreaHuen Co., Ltd., Gwanggyo Business Center 5F (#508), 156, Gwanggyo-ro, Yeongtong-gu, Suwon 16506, KoreaStem Cell Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, KoreaHuen Co., Ltd., Gwanggyo Business Center 5F (#508), 156, Gwanggyo-ro, Yeongtong-gu, Suwon 16506, KoreaStem Cell Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, KoreaParkinson’s disease (PD) is a well-known age-related neurodegenerative disease. Considering the vital importance of disease modeling based on reprogramming technology, we adopted direct reprogramming to human-induced neuronal progenitor cells (hiNPCs) for in vitro assessment of potential therapeutics. In this study, we investigated the neuroprotective effects of cryptotanshinone (CTN), which has been reported to have antioxidant properties, through PD patient-derived hiNPCs (PD-iNPCs) model with induced oxidative stress and cell death by the proteasome inhibitor MG132. A cytotoxicity assay showed that CTN possesses anti-apoptotic properties in PD-hiNPCs. CTN treatment significantly reduced cellular apoptosis through mitochondrial restoration, such as the reduction in mitochondrial reactive oxygen species and increments of mitochondrial membrane potential. These effects of CTN are mediated via the nuclear factor erythroid 2-related factor 2 (NRF2) pathway in PD-hiNPCs. Consequently, CTN could be a potential antioxidant reagent for preventing disease-related pathological phenotypes of PD.https://www.mdpi.com/1420-3049/25/16/3602Parkinson’s diseasecryptotanshinonedisease modelingmitochondrial dysfunctionantioxidant |
| spellingShingle | Joo-Eun Lee Hyuna Sim Hee Min Yoo Minhyung Lee Aruem Baek Young-Joo Jeon Kang-Sik Seo Mi-Young Son Joo Seog Yoon Janghwan Kim Neuroprotective Effects of Cryptotanshinone in a Direct Reprogramming Model of Parkinson’s Disease Molecules Parkinson’s disease cryptotanshinone disease modeling mitochondrial dysfunction antioxidant |
| title | Neuroprotective Effects of Cryptotanshinone in a Direct Reprogramming Model of Parkinson’s Disease |
| title_full | Neuroprotective Effects of Cryptotanshinone in a Direct Reprogramming Model of Parkinson’s Disease |
| title_fullStr | Neuroprotective Effects of Cryptotanshinone in a Direct Reprogramming Model of Parkinson’s Disease |
| title_full_unstemmed | Neuroprotective Effects of Cryptotanshinone in a Direct Reprogramming Model of Parkinson’s Disease |
| title_short | Neuroprotective Effects of Cryptotanshinone in a Direct Reprogramming Model of Parkinson’s Disease |
| title_sort | neuroprotective effects of cryptotanshinone in a direct reprogramming model of parkinson s disease |
| topic | Parkinson’s disease cryptotanshinone disease modeling mitochondrial dysfunction antioxidant |
| url | https://www.mdpi.com/1420-3049/25/16/3602 |
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