Hepatic morphological changes and oxidative stress in chronic streptozotocin-diabetic rats
Oxidative stress (OS) is a biological entity quoted as responsible for several pathologies including diabetes. Diabetes mellitus (DM) has been also associated to human cirrhosis. The present work was designed to study the occurrence of OS as well as morphologic alterations in rat livers following in...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2005-04-01
|
| Series: | Annals of Hepatology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1665268119320745 |
| _version_ | 1818596661614608384 |
|---|---|
| author | Pablo Evelson Carlota Susemihl Irene Villarreal Susana Llesuy Ricardo Rodríguez Horacio Peredo Abraham Lemberg Juan Perazzo Ester Filinger |
| author_facet | Pablo Evelson Carlota Susemihl Irene Villarreal Susana Llesuy Ricardo Rodríguez Horacio Peredo Abraham Lemberg Juan Perazzo Ester Filinger |
| author_sort | Pablo Evelson |
| collection | DOAJ |
| description | Oxidative stress (OS) is a biological entity quoted as responsible for several pathologies including diabetes. Diabetes mellitus (DM) has been also associated to human cirrhosis. The present work was designed to study the occurrence of OS as well as morphologic alterations in rat livers following induction of DM. Two groups of rats were used: Control and Diabetic. DM was induced in the second group by streptozotocin (STZ) in a single dose of 60 mg/kg, injected i.p. The occurrence of OS was determined in liver homogenates by measuring the hydroperoxide-initiated chemiluminescence and the activity of antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase). Liver sinusoids were morphometrically analyzed. In conclusion, livers from the diabetic group did not show evidence of the occurrence of OS, as it would be expected, but dilation of hepatic sinusoids was documented and it was significantly different from control group. |
| first_indexed | 2024-12-16T11:35:28Z |
| format | Article |
| id | doaj.art-b33e0444eaba442bab776d4d1ec4b3cd |
| institution | Directory Open Access Journal |
| issn | 1665-2681 |
| language | English |
| last_indexed | 2024-12-16T11:35:28Z |
| publishDate | 2005-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Annals of Hepatology |
| spelling | doaj.art-b33e0444eaba442bab776d4d1ec4b3cd2022-12-21T22:33:08ZengElsevierAnnals of Hepatology1665-26812005-04-0142115120Hepatic morphological changes and oxidative stress in chronic streptozotocin-diabetic ratsPablo Evelson0Carlota Susemihl1Irene Villarreal2Susana Llesuy3Ricardo Rodríguez4Horacio Peredo5Abraham Lemberg6Juan Perazzo7Ester Filinger8Laboratorio de Estrés Oxidativo en Patologías Humanas (LEOPH). Cátedra de Química General e Inorgánica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, ArgentinaCátedra de Farmacia Clínica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina, CONICETCátedra de Farmacia Clínica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina, CONICETLaboratorio de Estrés Oxidativo en Patologías Humanas (LEOPH). Cátedra de Química General e Inorgánica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, ArgentinaFacultad de Medicina. Universidad de El Salvador, Buenos Aires, ArgentinaCátedra de Farmacotecnia I. Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina, CONICETCátedra de Farmacotecnia I. Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina, CONICETCátedra de Fisiopatología. Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, ArgentinaCátedra de Farmacia Clínica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina, CONICET; Address for correspondence:Oxidative stress (OS) is a biological entity quoted as responsible for several pathologies including diabetes. Diabetes mellitus (DM) has been also associated to human cirrhosis. The present work was designed to study the occurrence of OS as well as morphologic alterations in rat livers following induction of DM. Two groups of rats were used: Control and Diabetic. DM was induced in the second group by streptozotocin (STZ) in a single dose of 60 mg/kg, injected i.p. The occurrence of OS was determined in liver homogenates by measuring the hydroperoxide-initiated chemiluminescence and the activity of antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase). Liver sinusoids were morphometrically analyzed. In conclusion, livers from the diabetic group did not show evidence of the occurrence of OS, as it would be expected, but dilation of hepatic sinusoids was documented and it was significantly different from control group.http://www.sciencedirect.com/science/article/pii/S1665268119320745Diabetesliverchemiluminescencefree radicalssinusoids |
| spellingShingle | Pablo Evelson Carlota Susemihl Irene Villarreal Susana Llesuy Ricardo Rodríguez Horacio Peredo Abraham Lemberg Juan Perazzo Ester Filinger Hepatic morphological changes and oxidative stress in chronic streptozotocin-diabetic rats Annals of Hepatology Diabetes liver chemiluminescence free radicals sinusoids |
| title | Hepatic morphological changes and oxidative stress in chronic streptozotocin-diabetic rats |
| title_full | Hepatic morphological changes and oxidative stress in chronic streptozotocin-diabetic rats |
| title_fullStr | Hepatic morphological changes and oxidative stress in chronic streptozotocin-diabetic rats |
| title_full_unstemmed | Hepatic morphological changes and oxidative stress in chronic streptozotocin-diabetic rats |
| title_short | Hepatic morphological changes and oxidative stress in chronic streptozotocin-diabetic rats |
| title_sort | hepatic morphological changes and oxidative stress in chronic streptozotocin diabetic rats |
| topic | Diabetes liver chemiluminescence free radicals sinusoids |
| url | http://www.sciencedirect.com/science/article/pii/S1665268119320745 |
| work_keys_str_mv | AT pabloevelson hepaticmorphologicalchangesandoxidativestressinchronicstreptozotocindiabeticrats AT carlotasusemihl hepaticmorphologicalchangesandoxidativestressinchronicstreptozotocindiabeticrats AT irenevillarreal hepaticmorphologicalchangesandoxidativestressinchronicstreptozotocindiabeticrats AT susanallesuy hepaticmorphologicalchangesandoxidativestressinchronicstreptozotocindiabeticrats AT ricardorodriguez hepaticmorphologicalchangesandoxidativestressinchronicstreptozotocindiabeticrats AT horacioperedo hepaticmorphologicalchangesandoxidativestressinchronicstreptozotocindiabeticrats AT abrahamlemberg hepaticmorphologicalchangesandoxidativestressinchronicstreptozotocindiabeticrats AT juanperazzo hepaticmorphologicalchangesandoxidativestressinchronicstreptozotocindiabeticrats AT esterfilinger hepaticmorphologicalchangesandoxidativestressinchronicstreptozotocindiabeticrats |