Investigating the Effect of Nano-Curcumin on the Expression of Biofilm Regulatory Genes of Pseudomonas aeruginosa
Parastoo Sharifian,1 Somayeh Yaslianifard,2 Parviz Fallah,3 Siavash Aynesazi,4 Mahmood Bakhtiyari,5 Mohammad Mohammadzadeh2 1Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran; 2Department of Microbiology, School of Medicine, Dietary Supplements and Pr...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2020-07-01
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| Series: | Infection and Drug Resistance |
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| Online Access: | https://www.dovepress.com/investigating-the-effect-of-nano-curcumin-on-the-expression-of-biofilm-peer-reviewed-article-IDR |
| _version_ | 1819016993666236416 |
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| author | Sharifian P Yaslianifard S Fallah P Aynesazi S Bakhtiyari M Mohammadzadeh M |
| author_facet | Sharifian P Yaslianifard S Fallah P Aynesazi S Bakhtiyari M Mohammadzadeh M |
| author_sort | Sharifian P |
| collection | DOAJ |
| description | Parastoo Sharifian,1 Somayeh Yaslianifard,2 Parviz Fallah,3 Siavash Aynesazi,4 Mahmood Bakhtiyari,5 Mohammad Mohammadzadeh2 1Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran; 2Department of Microbiology, School of Medicine, Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran; 3Department of Laboratory Science, Faculty of Allied Medicine, Alborz University of Medical Sciences, Karaj, Iran; 4Department of Microbiology, Faculty of Science, North Branch, Islamic Azad, Tehran, Iran; 5Department of Community Medicine and Epidemiology, School of Medicine, Non-Communicable Diseases Research Center, Alborz University of Medical Sciences, Karaj, IranCorrespondence: Mohammad Mohammadzadeh Tel +989126767995Email M.mohammadzadeh84@yahoo.comBackground: Pseudomonas aeruginosa is an opportunistic pathogen that causes serious nosocomial infections, especially in immunodeficient patients and cystic fibrosis, cancer, and burned individuals. The biofilm that plays an important role in the virulence of P. aeruginosa is under the regulation of quorum sensing and two-component regulatory systems of bacteria. Curcumin, an active phenolic extract of turmeric has shown an inhibitory effect on the biofilm formation of some pathogenic bacteria. Thus, the present study aims to evaluate the effect of Nano-Curcumin on the expression of major regulatory genes involved in biofilm formation of P. aeruginosa.Materials and Methods: The biofilm formation of P. aeruginosa ATCC 10145 was assessed in the presence of 15, 20, and 25 μg/mL concentrations of Nano-Curcumin using the microplate titer method. The effect of Nano-Curcumin on the expression level of regulatory genes were determined by relative reverse transcriptase-realtime PCR.Results: In the absence of Nano-Curcumin, P. aeruginosa strain ATCC 10145 strongly produced biofilm (3+) and in the presence of 15 and 20 μg/mL, biofilm formation was reduced to moderate (2+) and weak biofilm producer (1+), respectively. Nano-Curcumin at a concentration of 25μg/mL inhibited biofilm formation in P. aeruginosa. The expression of regulatory genes was not affected by biofilm inhibitory concentrations of Nano-Curcumin.Conclusion: The antibiofilm mechanism of Curcumin is not related to the downregulation of regulatory systems of P. aeruginosa and probably it prevents the formation of a complete biofilm structure.Keywords: Pseudomonas aeruginosa, biofilm formation, Nano-Curcumin |
| first_indexed | 2024-12-21T02:56:27Z |
| format | Article |
| id | doaj.art-b33e0996c3c742f7baf23ff3da68dcf3 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-12-21T02:56:27Z |
| publishDate | 2020-07-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-b33e0996c3c742f7baf23ff3da68dcf32022-12-21T19:18:19ZengDove Medical PressInfection and Drug Resistance1178-69732020-07-01Volume 132477248455500Investigating the Effect of Nano-Curcumin on the Expression of Biofilm Regulatory Genes of Pseudomonas aeruginosaSharifian PYaslianifard SFallah PAynesazi SBakhtiyari MMohammadzadeh MParastoo Sharifian,1 Somayeh Yaslianifard,2 Parviz Fallah,3 Siavash Aynesazi,4 Mahmood Bakhtiyari,5 Mohammad Mohammadzadeh2 1Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran; 2Department of Microbiology, School of Medicine, Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran; 3Department of Laboratory Science, Faculty of Allied Medicine, Alborz University of Medical Sciences, Karaj, Iran; 4Department of Microbiology, Faculty of Science, North Branch, Islamic Azad, Tehran, Iran; 5Department of Community Medicine and Epidemiology, School of Medicine, Non-Communicable Diseases Research Center, Alborz University of Medical Sciences, Karaj, IranCorrespondence: Mohammad Mohammadzadeh Tel +989126767995Email M.mohammadzadeh84@yahoo.comBackground: Pseudomonas aeruginosa is an opportunistic pathogen that causes serious nosocomial infections, especially in immunodeficient patients and cystic fibrosis, cancer, and burned individuals. The biofilm that plays an important role in the virulence of P. aeruginosa is under the regulation of quorum sensing and two-component regulatory systems of bacteria. Curcumin, an active phenolic extract of turmeric has shown an inhibitory effect on the biofilm formation of some pathogenic bacteria. Thus, the present study aims to evaluate the effect of Nano-Curcumin on the expression of major regulatory genes involved in biofilm formation of P. aeruginosa.Materials and Methods: The biofilm formation of P. aeruginosa ATCC 10145 was assessed in the presence of 15, 20, and 25 μg/mL concentrations of Nano-Curcumin using the microplate titer method. The effect of Nano-Curcumin on the expression level of regulatory genes were determined by relative reverse transcriptase-realtime PCR.Results: In the absence of Nano-Curcumin, P. aeruginosa strain ATCC 10145 strongly produced biofilm (3+) and in the presence of 15 and 20 μg/mL, biofilm formation was reduced to moderate (2+) and weak biofilm producer (1+), respectively. Nano-Curcumin at a concentration of 25μg/mL inhibited biofilm formation in P. aeruginosa. The expression of regulatory genes was not affected by biofilm inhibitory concentrations of Nano-Curcumin.Conclusion: The antibiofilm mechanism of Curcumin is not related to the downregulation of regulatory systems of P. aeruginosa and probably it prevents the formation of a complete biofilm structure.Keywords: Pseudomonas aeruginosa, biofilm formation, Nano-Curcuminhttps://www.dovepress.com/investigating-the-effect-of-nano-curcumin-on-the-expression-of-biofilm-peer-reviewed-article-IDRpseudomonas aeruginosabiofilm formationnano-curcumin |
| spellingShingle | Sharifian P Yaslianifard S Fallah P Aynesazi S Bakhtiyari M Mohammadzadeh M Investigating the Effect of Nano-Curcumin on the Expression of Biofilm Regulatory Genes of Pseudomonas aeruginosa Infection and Drug Resistance pseudomonas aeruginosa biofilm formation nano-curcumin |
| title | Investigating the Effect of Nano-Curcumin on the Expression of Biofilm Regulatory Genes of Pseudomonas aeruginosa |
| title_full | Investigating the Effect of Nano-Curcumin on the Expression of Biofilm Regulatory Genes of Pseudomonas aeruginosa |
| title_fullStr | Investigating the Effect of Nano-Curcumin on the Expression of Biofilm Regulatory Genes of Pseudomonas aeruginosa |
| title_full_unstemmed | Investigating the Effect of Nano-Curcumin on the Expression of Biofilm Regulatory Genes of Pseudomonas aeruginosa |
| title_short | Investigating the Effect of Nano-Curcumin on the Expression of Biofilm Regulatory Genes of Pseudomonas aeruginosa |
| title_sort | investigating the effect of nano curcumin on the expression of biofilm regulatory genes of pseudomonas aeruginosa |
| topic | pseudomonas aeruginosa biofilm formation nano-curcumin |
| url | https://www.dovepress.com/investigating-the-effect-of-nano-curcumin-on-the-expression-of-biofilm-peer-reviewed-article-IDR |
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