A novel sputum transport solution eliminates cold chain and supports routine tuberculosis testing in Nepal
This preliminary study evaluated the transport reagent OMNIgene SPUTUM (OMS) in a real-world, resource-limited setting: a zonal hospital and national tuberculosis (TB) reference laboratory, Nepal. The objectives were to: (1) assess the performance of OMS for transporting sputum from peripheral sites...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2019-04-01
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| Series: | Journal of Epidemiology and Global Health |
| Subjects: | |
| Online Access: | https://www.atlantis-press.com/article/125906070/view |
| _version_ | 1818475385145262080 |
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| author | Bhagwan Maharjan Bhabana Shrestha Alexandra Weirich Andrew Stewart Cassandra D. Kelly-Cirino |
| author_facet | Bhagwan Maharjan Bhabana Shrestha Alexandra Weirich Andrew Stewart Cassandra D. Kelly-Cirino |
| author_sort | Bhagwan Maharjan |
| collection | DOAJ |
| description | This preliminary study evaluated the transport reagent OMNIgene SPUTUM (OMS) in a real-world, resource-limited setting: a zonal hospital and national tuberculosis (TB) reference laboratory, Nepal. The objectives were to: (1) assess the performance of OMS for transporting sputum from peripheral sites without cold chain stabilization; and (2) compare with Nepal’s standard of care (SOC) for Mycobacterium tuberculosis smear and culture diagnostics. Sixty sputa were manually split into a SOC sample (airline-couriered to the laboratory, conventional processing) and an OMS sample (OMS added at collection, no cold chain transport or processing). Smear microscopy and solid culture were performed. Transport was 0–8 days. Forty-one samples (68%) were smear-positive using both methods. Of the OMS cultures, 37 (62%) were positive, 22 (36%) were negative, and one (2%) was contaminated. Corresponding SOC results were 32 (53%), 21 (35%), and seven (12%). OMS “rescued” six (i.e., missed using SOC) compared with one rescue using SOC. Of smear-positives, six SOC samples produced contaminated cultures whereas only one OMS sample was contaminated. OMS reduced culture contamination from 12% to 2%, and improved TB detection by 9%. The results suggest that OMS could perform well as a no cold chain, long-term transport solution for smear and culture testing. The findings provide a basis for larger feasibility studies. |
| first_indexed | 2024-12-10T09:11:52Z |
| format | Article |
| id | doaj.art-b34150995a1044f0ba563eac86079819 |
| institution | Directory Open Access Journal |
| issn | 2210-6006 |
| language | English |
| last_indexed | 2024-12-10T09:11:52Z |
| publishDate | 2019-04-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Epidemiology and Global Health |
| spelling | doaj.art-b34150995a1044f0ba563eac860798192022-12-22T01:55:00ZengSpringerJournal of Epidemiology and Global Health2210-60062019-04-016410.1016/j.jegh.2016.04.002A novel sputum transport solution eliminates cold chain and supports routine tuberculosis testing in NepalBhagwan MaharjanBhabana ShresthaAlexandra WeirichAndrew StewartCassandra D. Kelly-CirinoThis preliminary study evaluated the transport reagent OMNIgene SPUTUM (OMS) in a real-world, resource-limited setting: a zonal hospital and national tuberculosis (TB) reference laboratory, Nepal. The objectives were to: (1) assess the performance of OMS for transporting sputum from peripheral sites without cold chain stabilization; and (2) compare with Nepal’s standard of care (SOC) for Mycobacterium tuberculosis smear and culture diagnostics. Sixty sputa were manually split into a SOC sample (airline-couriered to the laboratory, conventional processing) and an OMS sample (OMS added at collection, no cold chain transport or processing). Smear microscopy and solid culture were performed. Transport was 0–8 days. Forty-one samples (68%) were smear-positive using both methods. Of the OMS cultures, 37 (62%) were positive, 22 (36%) were negative, and one (2%) was contaminated. Corresponding SOC results were 32 (53%), 21 (35%), and seven (12%). OMS “rescued” six (i.e., missed using SOC) compared with one rescue using SOC. Of smear-positives, six SOC samples produced contaminated cultures whereas only one OMS sample was contaminated. OMS reduced culture contamination from 12% to 2%, and improved TB detection by 9%. The results suggest that OMS could perform well as a no cold chain, long-term transport solution for smear and culture testing. The findings provide a basis for larger feasibility studies.https://www.atlantis-press.com/article/125906070/viewCulture contaminationLong-term sputum transportOMNIgene SPUTUM |
| spellingShingle | Bhagwan Maharjan Bhabana Shrestha Alexandra Weirich Andrew Stewart Cassandra D. Kelly-Cirino A novel sputum transport solution eliminates cold chain and supports routine tuberculosis testing in Nepal Journal of Epidemiology and Global Health Culture contamination Long-term sputum transport OMNIgene SPUTUM |
| title | A novel sputum transport solution eliminates cold chain and supports routine tuberculosis testing in Nepal |
| title_full | A novel sputum transport solution eliminates cold chain and supports routine tuberculosis testing in Nepal |
| title_fullStr | A novel sputum transport solution eliminates cold chain and supports routine tuberculosis testing in Nepal |
| title_full_unstemmed | A novel sputum transport solution eliminates cold chain and supports routine tuberculosis testing in Nepal |
| title_short | A novel sputum transport solution eliminates cold chain and supports routine tuberculosis testing in Nepal |
| title_sort | novel sputum transport solution eliminates cold chain and supports routine tuberculosis testing in nepal |
| topic | Culture contamination Long-term sputum transport OMNIgene SPUTUM |
| url | https://www.atlantis-press.com/article/125906070/view |
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