UPLC Quantitative Analysis of Multi-Components by Single Marker and Quality Evaluation of Polygala tenuifolia Wild. Extracts
The quality control of Polygala tenuifolia Wild. is a major challenge in its clinical application. In this paper, a new strategy for the quality evaluation of P. tenuifolia extracts was verified through reverse-phase ultra-performance liquid chromatography (UPLC). The quantitative analysis of multi-...
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MDPI AG
2017-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/22/12/2276 |
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| author | Rui Xu Fuying Mao Yunsheng Zhao Wenping Wang Lingling Fan Xiaojuan Gao Jianjun Zhao Hongling Tian |
| author_facet | Rui Xu Fuying Mao Yunsheng Zhao Wenping Wang Lingling Fan Xiaojuan Gao Jianjun Zhao Hongling Tian |
| author_sort | Rui Xu |
| collection | DOAJ |
| description | The quality control of Polygala tenuifolia Wild. is a major challenge in its clinical application. In this paper, a new strategy for the quality evaluation of P. tenuifolia extracts was verified through reverse-phase ultra-performance liquid chromatography (UPLC). The quantitative analysis of multi-components by a single marker (QAMS) was conducted with 3,6′-disinapoyl sucrose as an internal reference substance. Eight components (i.e., sibiricose A5, sibiricose A6, glomeratose A, tenuifoliside A, tenuifoliside B, tenuifoliside C, sibiricaxanthone B, and polygalaxanthone III) were determined based on the relative correction factors. The concentrations of these components were also determined by applying a conventional external standard method. The cosine value confirmed the consistency of the two methods (cosine ratio value >0.999920). Hierarchical cluster analysis, radar plots, and discriminant analysis were performed to classify 23 batches of P. tenuifolia extracts from Shanxi, Hebei, and Shaanxi in China. Results revealed that QAMS combined with radar plots and multivariate data analysis could accurately measure and clearly distinguish the different quality samples of P. tenuifolia. Hence, QAMS is a feasible and promising method for the quality control of P. tenuifolia. |
| first_indexed | 2024-12-19T23:14:01Z |
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| language | English |
| last_indexed | 2024-12-19T23:14:01Z |
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| spelling | doaj.art-b341865965a64b21b45e8c566dafd7912022-12-21T20:02:08ZengMDPI AGMolecules1420-30492017-12-012212227610.3390/molecules22122276molecules22122276UPLC Quantitative Analysis of Multi-Components by Single Marker and Quality Evaluation of Polygala tenuifolia Wild. ExtractsRui Xu0Fuying Mao1Yunsheng Zhao2Wenping Wang3Lingling Fan4Xiaojuan Gao5Jianjun Zhao6Hongling Tian7School of Pharmacy, Ningxia Medical University, Yinchuan 750004, Ningxia, ChinaSchool of Pharmacy, Ningxia Medical University, Yinchuan 750004, Ningxia, ChinaSchool of Pharmacy, Ningxia Medical University, Yinchuan 750004, Ningxia, ChinaSchool of Pharmacy, Ningxia Medical University, Yinchuan 750004, Ningxia, ChinaSchool of Pharmacy, Ningxia Medical University, Yinchuan 750004, Ningxia, ChinaSchool of Pharmacy, Ningxia Medical University, Yinchuan 750004, Ningxia, ChinaSchool of Pharmacy, Ningxia Medical University, Yinchuan 750004, Ningxia, ChinaInstitute of Industrial Crop Research, Shanxi Academy of Agricultural Sciences, Fenyang 032200, Shanxi, ChinaThe quality control of Polygala tenuifolia Wild. is a major challenge in its clinical application. In this paper, a new strategy for the quality evaluation of P. tenuifolia extracts was verified through reverse-phase ultra-performance liquid chromatography (UPLC). The quantitative analysis of multi-components by a single marker (QAMS) was conducted with 3,6′-disinapoyl sucrose as an internal reference substance. Eight components (i.e., sibiricose A5, sibiricose A6, glomeratose A, tenuifoliside A, tenuifoliside B, tenuifoliside C, sibiricaxanthone B, and polygalaxanthone III) were determined based on the relative correction factors. The concentrations of these components were also determined by applying a conventional external standard method. The cosine value confirmed the consistency of the two methods (cosine ratio value >0.999920). Hierarchical cluster analysis, radar plots, and discriminant analysis were performed to classify 23 batches of P. tenuifolia extracts from Shanxi, Hebei, and Shaanxi in China. Results revealed that QAMS combined with radar plots and multivariate data analysis could accurately measure and clearly distinguish the different quality samples of P. tenuifolia. Hence, QAMS is a feasible and promising method for the quality control of P. tenuifolia.https://www.mdpi.com/1420-3049/22/12/2276Polygala tenuifolia Wild.multi-components by single markerultra-performance liquid chromatographyquality evaluation |
| spellingShingle | Rui Xu Fuying Mao Yunsheng Zhao Wenping Wang Lingling Fan Xiaojuan Gao Jianjun Zhao Hongling Tian UPLC Quantitative Analysis of Multi-Components by Single Marker and Quality Evaluation of Polygala tenuifolia Wild. Extracts Molecules Polygala tenuifolia Wild. multi-components by single marker ultra-performance liquid chromatography quality evaluation |
| title | UPLC Quantitative Analysis of Multi-Components by Single Marker and Quality Evaluation of Polygala tenuifolia Wild. Extracts |
| title_full | UPLC Quantitative Analysis of Multi-Components by Single Marker and Quality Evaluation of Polygala tenuifolia Wild. Extracts |
| title_fullStr | UPLC Quantitative Analysis of Multi-Components by Single Marker and Quality Evaluation of Polygala tenuifolia Wild. Extracts |
| title_full_unstemmed | UPLC Quantitative Analysis of Multi-Components by Single Marker and Quality Evaluation of Polygala tenuifolia Wild. Extracts |
| title_short | UPLC Quantitative Analysis of Multi-Components by Single Marker and Quality Evaluation of Polygala tenuifolia Wild. Extracts |
| title_sort | uplc quantitative analysis of multi components by single marker and quality evaluation of polygala tenuifolia wild extracts |
| topic | Polygala tenuifolia Wild. multi-components by single marker ultra-performance liquid chromatography quality evaluation |
| url | https://www.mdpi.com/1420-3049/22/12/2276 |
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