Cloning, Expression and Characterization of a Thermostable Esterase HydS14 from Actinomadura sp. Strain S14 in Pichia pastoris
A thermostable esterase gene (hydS14) was cloned from an Actinomadura sp. S14 gene library. The gene is 777 bp in length and encodes a polypeptide of 258 amino acid residues with no signal peptide, no N-glycosylation site and a predicted molecular mass of 26,604 Da. The encoded protein contains the...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2015-06-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1422-0067/16/6/13579 |
| _version_ | 1811263175915470848 |
|---|---|
| author | Pichapak Sriyapai Fusako Kawai Somjai Siripoke Kosum Chansiri Thayat Sriyapai |
| author_facet | Pichapak Sriyapai Fusako Kawai Somjai Siripoke Kosum Chansiri Thayat Sriyapai |
| author_sort | Pichapak Sriyapai |
| collection | DOAJ |
| description | A thermostable esterase gene (hydS14) was cloned from an Actinomadura sp. S14 gene library. The gene is 777 bp in length and encodes a polypeptide of 258 amino acid residues with no signal peptide, no N-glycosylation site and a predicted molecular mass of 26,604 Da. The encoded protein contains the pentapeptide motif (GYSLG) and catalytic triad (Ser88-Asp208-His235) of the esterase/lipase superfamily. The HydS14 sequence shows 46%–64% identity to 23 sequences from actinomycetes (23 α/β-hydrolases), has three conserved regions, and contains the novel motif (GY(F)SLG), which distinguishes it from other clusters in the α/β-hydrolase structural superfamily. A plasmid containing the coding region (pPICZαA-hydS14) was used to express HydS14 in Pichia pastoris under the control of the AOXI promoter. The recombinant HydS14 collected from the supernatant had a molecular mass of ~30 kDa, which agrees with its predicted molecular mass without N-glycosylation. HydS14 had an optimum temperature of approximately 70 °C and an optimum pH of 8.0. HydS14 was stable at 50 and 60 °C for 120 min, with residual activities of above 80% and above 90%, respectively, as well as 50% activity at pH 6.0–8.0 and pH 9.0, respectively. The enzyme showed higher activity with p-nitrophenyl-C2 and C4. The Km and Vmax values for p-nitrophenyl-C4 were 0.21 ± 0.02 mM and 37.07 ± 1.04 μmol/min/mg, respectively. The enzyme was active toward short-chain p-nitrophenyl ester (C2–C6), displaying optimal activity with p-nitrophenyl-C4 (Kcat/Km = 11.74 mM−1·S−1). In summary, HydS14 is a thermostable esterase from Actinomadura sp. S14 that has been cloned and expressed for the first time in Pichia pastoris. |
| first_indexed | 2024-04-12T19:39:58Z |
| format | Article |
| id | doaj.art-b3427009ed8e422e971f2c316632e48a |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T19:39:58Z |
| publishDate | 2015-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b3427009ed8e422e971f2c316632e48a2022-12-22T03:19:06ZengMDPI AGInternational Journal of Molecular Sciences1422-00672015-06-01166135791359410.3390/ijms160613579ijms160613579Cloning, Expression and Characterization of a Thermostable Esterase HydS14 from Actinomadura sp. Strain S14 in Pichia pastorisPichapak Sriyapai0Fusako Kawai1Somjai Siripoke2Kosum Chansiri3Thayat Sriyapai4Department of Biology, Faculty of Sciences, Srinakharinwirot University, Bangkok 10110, ThailandCenter for Nanomaterials and Devices, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, JapanInnovative Learning Center, Srinakharinwirot University, Bangkok 10110, ThailandDepartment of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, ThailandFaculty of Environmental Culture and Ecotourism, Srinakharinwirot University, Bangkok 10110, ThailandA thermostable esterase gene (hydS14) was cloned from an Actinomadura sp. S14 gene library. The gene is 777 bp in length and encodes a polypeptide of 258 amino acid residues with no signal peptide, no N-glycosylation site and a predicted molecular mass of 26,604 Da. The encoded protein contains the pentapeptide motif (GYSLG) and catalytic triad (Ser88-Asp208-His235) of the esterase/lipase superfamily. The HydS14 sequence shows 46%–64% identity to 23 sequences from actinomycetes (23 α/β-hydrolases), has three conserved regions, and contains the novel motif (GY(F)SLG), which distinguishes it from other clusters in the α/β-hydrolase structural superfamily. A plasmid containing the coding region (pPICZαA-hydS14) was used to express HydS14 in Pichia pastoris under the control of the AOXI promoter. The recombinant HydS14 collected from the supernatant had a molecular mass of ~30 kDa, which agrees with its predicted molecular mass without N-glycosylation. HydS14 had an optimum temperature of approximately 70 °C and an optimum pH of 8.0. HydS14 was stable at 50 and 60 °C for 120 min, with residual activities of above 80% and above 90%, respectively, as well as 50% activity at pH 6.0–8.0 and pH 9.0, respectively. The enzyme showed higher activity with p-nitrophenyl-C2 and C4. The Km and Vmax values for p-nitrophenyl-C4 were 0.21 ± 0.02 mM and 37.07 ± 1.04 μmol/min/mg, respectively. The enzyme was active toward short-chain p-nitrophenyl ester (C2–C6), displaying optimal activity with p-nitrophenyl-C4 (Kcat/Km = 11.74 mM−1·S−1). In summary, HydS14 is a thermostable esterase from Actinomadura sp. S14 that has been cloned and expressed for the first time in Pichia pastoris.http://www.mdpi.com/1422-0067/16/6/13579Actinomadura sp.esteraseα/β-hydrolase superfamilylipolytic familythermophile |
| spellingShingle | Pichapak Sriyapai Fusako Kawai Somjai Siripoke Kosum Chansiri Thayat Sriyapai Cloning, Expression and Characterization of a Thermostable Esterase HydS14 from Actinomadura sp. Strain S14 in Pichia pastoris International Journal of Molecular Sciences Actinomadura sp. esterase α/β-hydrolase superfamily lipolytic family thermophile |
| title | Cloning, Expression and Characterization of a Thermostable Esterase HydS14 from Actinomadura sp. Strain S14 in Pichia pastoris |
| title_full | Cloning, Expression and Characterization of a Thermostable Esterase HydS14 from Actinomadura sp. Strain S14 in Pichia pastoris |
| title_fullStr | Cloning, Expression and Characterization of a Thermostable Esterase HydS14 from Actinomadura sp. Strain S14 in Pichia pastoris |
| title_full_unstemmed | Cloning, Expression and Characterization of a Thermostable Esterase HydS14 from Actinomadura sp. Strain S14 in Pichia pastoris |
| title_short | Cloning, Expression and Characterization of a Thermostable Esterase HydS14 from Actinomadura sp. Strain S14 in Pichia pastoris |
| title_sort | cloning expression and characterization of a thermostable esterase hyds14 from actinomadura sp strain s14 in pichia pastoris |
| topic | Actinomadura sp. esterase α/β-hydrolase superfamily lipolytic family thermophile |
| url | http://www.mdpi.com/1422-0067/16/6/13579 |
| work_keys_str_mv | AT pichapaksriyapai cloningexpressionandcharacterizationofathermostableesterasehyds14fromactinomaduraspstrains14inpichiapastoris AT fusakokawai cloningexpressionandcharacterizationofathermostableesterasehyds14fromactinomaduraspstrains14inpichiapastoris AT somjaisiripoke cloningexpressionandcharacterizationofathermostableesterasehyds14fromactinomaduraspstrains14inpichiapastoris AT kosumchansiri cloningexpressionandcharacterizationofathermostableesterasehyds14fromactinomaduraspstrains14inpichiapastoris AT thayatsriyapai cloningexpressionandcharacterizationofathermostableesterasehyds14fromactinomaduraspstrains14inpichiapastoris |