PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei
The genetic variation in two farmed strains (F3-Panama and F17-Venezuela) of the shrimp Litopenaeus vannamei was examined based on DNA multiloci analyses. Eighteen adults of each strain were analyzed by PCR using a set of VNTR core sequence primers. Genetic similarity, mean allele frequency, mean he...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2002-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400012 |
| _version_ | 1818339955610484736 |
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| author | Patrícia Domingues de Freitas Pedro Manoel Galetti Junior |
| author_facet | Patrícia Domingues de Freitas Pedro Manoel Galetti Junior |
| author_sort | Patrícia Domingues de Freitas |
| collection | DOAJ |
| description | The genetic variation in two farmed strains (F3-Panama and F17-Venezuela) of the shrimp Litopenaeus vannamei was examined based on DNA multiloci analyses. Eighteen adults of each strain were analyzed by PCR using a set of VNTR core sequence primers. Genetic similarity, mean allele frequency, mean heterozygosity and the frequency of polymorphic loci were determined for both strains. A dendrogram of genetic similarity was produced by UPGMA clustering. The results for three primers (INS, M13, YN73) revealed different levels of genetic variation within the strains. The higher genetic similarity seen within strain F17 was apparently related to inbreeding, although a bottleneck effect could not be discarded. The low level of genetic variability of this strain could account for the reduced adaptive advantage of these animals and their inability to adjust to breeding conditions in Brazil. |
| first_indexed | 2024-12-13T15:35:14Z |
| format | Article |
| id | doaj.art-b344b32f5dc14602b582b7ba98ba7069 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-13T15:35:14Z |
| publishDate | 2002-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-b344b32f5dc14602b582b7ba98ba70692022-12-21T23:40:04ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852002-01-0125443143410.1590/S1415-47572002000400012PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannameiPatrícia Domingues de FreitasPedro Manoel Galetti JuniorThe genetic variation in two farmed strains (F3-Panama and F17-Venezuela) of the shrimp Litopenaeus vannamei was examined based on DNA multiloci analyses. Eighteen adults of each strain were analyzed by PCR using a set of VNTR core sequence primers. Genetic similarity, mean allele frequency, mean heterozygosity and the frequency of polymorphic loci were determined for both strains. A dendrogram of genetic similarity was produced by UPGMA clustering. The results for three primers (INS, M13, YN73) revealed different levels of genetic variation within the strains. The higher genetic similarity seen within strain F17 was apparently related to inbreeding, although a bottleneck effect could not be discarded. The low level of genetic variability of this strain could account for the reduced adaptive advantage of these animals and their inability to adjust to breeding conditions in Brazil.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400012Litopenaeus vannameigenetic diversityshrimpVNTRPCR |
| spellingShingle | Patrícia Domingues de Freitas Pedro Manoel Galetti Junior PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei Genetics and Molecular Biology Litopenaeus vannamei genetic diversity shrimp VNTR PCR |
| title | PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei |
| title_full | PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei |
| title_fullStr | PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei |
| title_full_unstemmed | PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei |
| title_short | PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei |
| title_sort | pcr based vntr core sequence analysis for inferring genetic diversity in the shrimp litopenaeus vannamei |
| topic | Litopenaeus vannamei genetic diversity shrimp VNTR PCR |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400012 |
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