Analysis of cross neutralizing activity of antibodies from sera of severe fever with thrombocytopenia syndrome patients to deal with different genotype strains
BackgroundSevere fever with thrombocytopenia syndrome bunyavirus (SFTSV) is a tick-borne virus that causes severe communicable fever with thrombocytopenia syndrome (SFTS) with an average case fatality rate of 10%. In the study, we aimed to identify the cross-neutralizing antibody (nAb) against diffe...
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1020545/full |
| _version_ | 1817978846291427328 |
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| author | Jiaxin Xu Yuanni Liu Fan Zhang Xin Wang Weijin Huang Yulong Wu Boqing Li Jiapeng Zhuang Yixing Bing Youchun Wang Yuanyuan Qiao |
| author_facet | Jiaxin Xu Yuanni Liu Fan Zhang Xin Wang Weijin Huang Yulong Wu Boqing Li Jiapeng Zhuang Yixing Bing Youchun Wang Yuanyuan Qiao |
| author_sort | Jiaxin Xu |
| collection | DOAJ |
| description | BackgroundSevere fever with thrombocytopenia syndrome bunyavirus (SFTSV) is a tick-borne virus that causes severe communicable fever with thrombocytopenia syndrome (SFTS) with an average case fatality rate of 10%. In the study, we aimed to identify the cross-neutralizing antibody (nAb) against different genotype strains from sera of SFTSV infected patients.MethodsFirstly the genotype of SFTSV was identified by constructing a phylogenetic tree based on the M segments epidemic in the Jiaodong area of Shandong province, then different sera of subjects cross reactive with recombinant Gn (rGn-Fc) or recombinant Gc (rGc-Fc) of 0921 strain were examined. The levels of polyclonal nAbs from sera of 25 convalescents were measured by a pseudovirus-based neutralizing experiment.ResultsWe found local endemic strains were mainly C2 and C3 isolates of SFTSV. 14 of 15 sera from donors reacted with 0921 rGn-Fc, and 9 of 15 sera from donors reacted with 0921 rGc-Fc. Cross nAbs were produced by 10 of 25 sera from donors during the period of 2019–2021. Among these, five nAbs (A2, A4, A5, L9, and L10) neutralized the pseudoviruses of HB29, Gangwon, HN13, HN20, SPL030A, and SD4 strains.ConclusionOur data suggested that epidemic strains showed relatively stable heredity. Some blood sources from patients produced cross nAbs that could neutralize all of the strains examined. These findings highlight the important role played by humoral immunity in combatting SFTSV. |
| first_indexed | 2024-04-13T22:35:34Z |
| format | Article |
| id | doaj.art-b345eb6565844bf6886022a98b0c1fa9 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T22:35:34Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-b345eb6565844bf6886022a98b0c1fa92022-12-22T02:26:47ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-10-011310.3389/fmicb.2022.10205451020545Analysis of cross neutralizing activity of antibodies from sera of severe fever with thrombocytopenia syndrome patients to deal with different genotype strainsJiaxin Xu0Yuanni Liu1Fan Zhang2Xin Wang3Weijin Huang4Yulong Wu5Boqing Li6Jiapeng Zhuang7Yixing Bing8Youchun Wang9Yuanyuan Qiao10Department of Pathogenic Biology, Binzhou Medical University, Yantai, ChinaDepartment of Infectious Diseases and Clinical Laboratory, Yantai Qishan Hospital, Yantai, ChinaDepartment of Pathogenic Biology, Binzhou Medical University, Yantai, ChinaDepartment of Infectious Diseases and Clinical Laboratory, Yantai Qishan Hospital, Yantai, ChinaKey Laboratory for Quality Research and Evaluation of Biological Products, National Medical Products Administration, Beijing, ChinaDepartment of Pathogenic Biology, Binzhou Medical University, Yantai, ChinaDepartment of Pathogenic Biology, Binzhou Medical University, Yantai, ChinaDepartment of Pathogenic Biology, Binzhou Medical University, Yantai, ChinaDepartment of Pathogenic Biology, Binzhou Medical University, Yantai, ChinaKey Laboratory for Quality Research and Evaluation of Biological Products, National Medical Products Administration, Beijing, ChinaDepartment of Pathogenic Biology, Binzhou Medical University, Yantai, ChinaBackgroundSevere fever with thrombocytopenia syndrome bunyavirus (SFTSV) is a tick-borne virus that causes severe communicable fever with thrombocytopenia syndrome (SFTS) with an average case fatality rate of 10%. In the study, we aimed to identify the cross-neutralizing antibody (nAb) against different genotype strains from sera of SFTSV infected patients.MethodsFirstly the genotype of SFTSV was identified by constructing a phylogenetic tree based on the M segments epidemic in the Jiaodong area of Shandong province, then different sera of subjects cross reactive with recombinant Gn (rGn-Fc) or recombinant Gc (rGc-Fc) of 0921 strain were examined. The levels of polyclonal nAbs from sera of 25 convalescents were measured by a pseudovirus-based neutralizing experiment.ResultsWe found local endemic strains were mainly C2 and C3 isolates of SFTSV. 14 of 15 sera from donors reacted with 0921 rGn-Fc, and 9 of 15 sera from donors reacted with 0921 rGc-Fc. Cross nAbs were produced by 10 of 25 sera from donors during the period of 2019–2021. Among these, five nAbs (A2, A4, A5, L9, and L10) neutralized the pseudoviruses of HB29, Gangwon, HN13, HN20, SPL030A, and SD4 strains.ConclusionOur data suggested that epidemic strains showed relatively stable heredity. Some blood sources from patients produced cross nAbs that could neutralize all of the strains examined. These findings highlight the important role played by humoral immunity in combatting SFTSV.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1020545/fullsevere fever with thrombocytopenia syndrome bunyavirushumoral immunityneutralizing antibodyglycoproteinphylogenetic tree |
| spellingShingle | Jiaxin Xu Yuanni Liu Fan Zhang Xin Wang Weijin Huang Yulong Wu Boqing Li Jiapeng Zhuang Yixing Bing Youchun Wang Yuanyuan Qiao Analysis of cross neutralizing activity of antibodies from sera of severe fever with thrombocytopenia syndrome patients to deal with different genotype strains Frontiers in Microbiology severe fever with thrombocytopenia syndrome bunyavirus humoral immunity neutralizing antibody glycoprotein phylogenetic tree |
| title | Analysis of cross neutralizing activity of antibodies from sera of severe fever with thrombocytopenia syndrome patients to deal with different genotype strains |
| title_full | Analysis of cross neutralizing activity of antibodies from sera of severe fever with thrombocytopenia syndrome patients to deal with different genotype strains |
| title_fullStr | Analysis of cross neutralizing activity of antibodies from sera of severe fever with thrombocytopenia syndrome patients to deal with different genotype strains |
| title_full_unstemmed | Analysis of cross neutralizing activity of antibodies from sera of severe fever with thrombocytopenia syndrome patients to deal with different genotype strains |
| title_short | Analysis of cross neutralizing activity of antibodies from sera of severe fever with thrombocytopenia syndrome patients to deal with different genotype strains |
| title_sort | analysis of cross neutralizing activity of antibodies from sera of severe fever with thrombocytopenia syndrome patients to deal with different genotype strains |
| topic | severe fever with thrombocytopenia syndrome bunyavirus humoral immunity neutralizing antibody glycoprotein phylogenetic tree |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1020545/full |
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