Evaluation of chemokines and receptors in gnotobiotic root canal infection by F. nucleatum and E. faecalis
Abstract The present study aims to evaluate the longitudinal effects of induced experimental infections in gnotoxenic animals on the expression of inflammatory chemokines and their receptors in periradicular tissues. The null hypothesis tested was that Enterococcus faecalis and Fusobacterium nucleat...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Pesquisa Odontológica
2018-11-01
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| Series: | Brazilian Oral Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100296&lng=en&tlng=en |
| _version_ | 1818483684487987200 |
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| author | Caroline Christine Santa-Rosa Marcela Marçal Thebit Kamilla Faria Maciel Luciana Carla Neves de Brito Leda Quercia Vieira Antônio Paulino Ribeiro-Sobrinho |
| author_facet | Caroline Christine Santa-Rosa Marcela Marçal Thebit Kamilla Faria Maciel Luciana Carla Neves de Brito Leda Quercia Vieira Antônio Paulino Ribeiro-Sobrinho |
| author_sort | Caroline Christine Santa-Rosa |
| collection | DOAJ |
| description | Abstract The present study aims to evaluate the longitudinal effects of induced experimental infections in gnotoxenic animals on the expression of inflammatory chemokines and their receptors in periradicular tissues. The null hypothesis tested was that Enterococcus faecalis and Fusobacterium nucleatum had no effect on CCR5, CCL5, CXCL10, CCL2/MCP-1, CXCR2 and CCR1 expression. Two groups of five animals (n = 5) aged between 8 and 12 weeks were used in this study. The animals were anaesthetized, and coronary access was performed in the first molar on the right and left sides. Microorganisms were inoculated into the left molar, and the right molar was sealed without contamination to function as a control. Animals were sacrificed 7 and 14 days after infection, and periapical tissues were collected. The cytokine mRNA expression levels were assessed using real-time PCR. The chemokine mRNA expression levels demonstrated that the experimental infection was capable of inducing increased chemokine expression on day 7 compared to that on day 14, except for CCR5 and CCL5, which showed no changes. The gnotoxenic animal model proved to be effective and allowed evaluation of the immune response against a known infection. Additionally, this study demonstrates that gene expression of chemokines and their receptors against the experimental infection preferentially prevailed during the initial phase of induction of the periradicular alteration (i.e., on day 7 post-infection). |
| first_indexed | 2024-12-10T15:45:10Z |
| format | Article |
| id | doaj.art-b34824591f7a4b609676a486c779d432 |
| institution | Directory Open Access Journal |
| issn | 1807-3107 |
| language | English |
| last_indexed | 2024-12-10T15:45:10Z |
| publishDate | 2018-11-01 |
| publisher | Sociedade Brasileira de Pesquisa Odontológica |
| record_format | Article |
| series | Brazilian Oral Research |
| spelling | doaj.art-b34824591f7a4b609676a486c779d4322022-12-22T01:42:58ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1807-31072018-11-0132010.1590/1807-3107bor-2018.vol32.0120S1806-83242018000100296Evaluation of chemokines and receptors in gnotobiotic root canal infection by F. nucleatum and E. faecalisCaroline Christine Santa-RosaMarcela Marçal ThebitKamilla Faria MacielLuciana Carla Neves de BritoLeda Quercia VieiraAntônio Paulino Ribeiro-SobrinhoAbstract The present study aims to evaluate the longitudinal effects of induced experimental infections in gnotoxenic animals on the expression of inflammatory chemokines and their receptors in periradicular tissues. The null hypothesis tested was that Enterococcus faecalis and Fusobacterium nucleatum had no effect on CCR5, CCL5, CXCL10, CCL2/MCP-1, CXCR2 and CCR1 expression. Two groups of five animals (n = 5) aged between 8 and 12 weeks were used in this study. The animals were anaesthetized, and coronary access was performed in the first molar on the right and left sides. Microorganisms were inoculated into the left molar, and the right molar was sealed without contamination to function as a control. Animals were sacrificed 7 and 14 days after infection, and periapical tissues were collected. The cytokine mRNA expression levels were assessed using real-time PCR. The chemokine mRNA expression levels demonstrated that the experimental infection was capable of inducing increased chemokine expression on day 7 compared to that on day 14, except for CCR5 and CCL5, which showed no changes. The gnotoxenic animal model proved to be effective and allowed evaluation of the immune response against a known infection. Additionally, this study demonstrates that gene expression of chemokines and their receptors against the experimental infection preferentially prevailed during the initial phase of induction of the periradicular alteration (i.e., on day 7 post-infection).http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100296&lng=en&tlng=enGerm-free LifeChemonikesPeriapical DiseasesMicrobiology |
| spellingShingle | Caroline Christine Santa-Rosa Marcela Marçal Thebit Kamilla Faria Maciel Luciana Carla Neves de Brito Leda Quercia Vieira Antônio Paulino Ribeiro-Sobrinho Evaluation of chemokines and receptors in gnotobiotic root canal infection by F. nucleatum and E. faecalis Brazilian Oral Research Germ-free Life Chemonikes Periapical Diseases Microbiology |
| title | Evaluation of chemokines and receptors in gnotobiotic root canal infection by F. nucleatum and E. faecalis |
| title_full | Evaluation of chemokines and receptors in gnotobiotic root canal infection by F. nucleatum and E. faecalis |
| title_fullStr | Evaluation of chemokines and receptors in gnotobiotic root canal infection by F. nucleatum and E. faecalis |
| title_full_unstemmed | Evaluation of chemokines and receptors in gnotobiotic root canal infection by F. nucleatum and E. faecalis |
| title_short | Evaluation of chemokines and receptors in gnotobiotic root canal infection by F. nucleatum and E. faecalis |
| title_sort | evaluation of chemokines and receptors in gnotobiotic root canal infection by f nucleatum and e faecalis |
| topic | Germ-free Life Chemonikes Periapical Diseases Microbiology |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100296&lng=en&tlng=en |
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