Green tea increases the survival yield of Bifidobacteria in simulated gastrointestinal environment and during refrigerated conditions
<p>Abstract</p> <p>Background</p> <p>The well–known prebiotics are carbohydrates but their effects may not always be beneficial, as they can also encourage the growth of non-probiotic bacteria such as <it>Eubacterium biforme</it> and <it>Clostridium pe...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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BMC
2012-06-01
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| Series: | Chemistry Central Journal |
| Subjects: | |
| Online Access: | http://journal.chemistrycentral.com/content/6/1/61 |
| _version_ | 1818872419398451200 |
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| author | Vodnar Dan C Socaciu Carmen |
| author_facet | Vodnar Dan C Socaciu Carmen |
| author_sort | Vodnar Dan C |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The well–known prebiotics are carbohydrates but their effects may not always be beneficial, as they can also encourage the growth of non-probiotic bacteria such as <it>Eubacterium biforme</it> and <it>Clostridium perfringens</it>. Therefore, new alternatives such as non-carbohydrate sources to stimulate the growth of probiotics are needed. The aim of this work was to evaluate (I) the green tea polyphenols by HPLC-LC/MS and (II) the protective effect of green tea extract on viability and stability of <it>B. infantis ATCC 15697</it> and <it>B. breve</it> ATCC 15700 microencapsulated in chitosan coated alginate microcapsules during exposure to simulated gastrointestinal conditions and refrigerated storage.</p> <p>Results</p> <p>The major compound identified by HPLC-LC/MS in green tea was epigallocatechin gallate followed by caffeine and epigallocatechin. The survival yield of probiotic bacteria in microcapsules with 10% GT during storage at 4°C, demonstrated significantly (P < 0.05) higher number of survival bacteria. Microencapsulated <it>B.infantis</it> and <it>B. breve</it> with 5% and 10% GT showed a significantly (P < 0.05) improved survival under simulated gastric conditions (pH 2.0, 2 h) and bile solution (3%, 2 h) when they were compared with microencapsulation without GT addition.</p> <p>Conclusions</p> <p>The results of this study suggest that green tea coencapsulated with <it>B. infantis</it> or <it>B. breve</it> exert a protective effect of bacteria during exposure to gastrointestinal conditions and refrigerated storage. For a health perspective, the results confirm the growing interest probiotic bacteria and the perceived benefit of increasing their numbers in the gastrointestinal tract by microencapsulation.</p> |
| first_indexed | 2024-12-19T12:38:31Z |
| format | Article |
| id | doaj.art-b34bb20bedbe45999eb6a6d27741e718 |
| institution | Directory Open Access Journal |
| issn | 1752-153X |
| language | English |
| last_indexed | 2024-12-19T12:38:31Z |
| publishDate | 2012-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Chemistry Central Journal |
| spelling | doaj.art-b34bb20bedbe45999eb6a6d27741e7182022-12-21T20:21:04ZengBMCChemistry Central Journal1752-153X2012-06-01616110.1186/1752-153X-6-61Green tea increases the survival yield of Bifidobacteria in simulated gastrointestinal environment and during refrigerated conditionsVodnar Dan CSocaciu Carmen<p>Abstract</p> <p>Background</p> <p>The well–known prebiotics are carbohydrates but their effects may not always be beneficial, as they can also encourage the growth of non-probiotic bacteria such as <it>Eubacterium biforme</it> and <it>Clostridium perfringens</it>. Therefore, new alternatives such as non-carbohydrate sources to stimulate the growth of probiotics are needed. The aim of this work was to evaluate (I) the green tea polyphenols by HPLC-LC/MS and (II) the protective effect of green tea extract on viability and stability of <it>B. infantis ATCC 15697</it> and <it>B. breve</it> ATCC 15700 microencapsulated in chitosan coated alginate microcapsules during exposure to simulated gastrointestinal conditions and refrigerated storage.</p> <p>Results</p> <p>The major compound identified by HPLC-LC/MS in green tea was epigallocatechin gallate followed by caffeine and epigallocatechin. The survival yield of probiotic bacteria in microcapsules with 10% GT during storage at 4°C, demonstrated significantly (P < 0.05) higher number of survival bacteria. Microencapsulated <it>B.infantis</it> and <it>B. breve</it> with 5% and 10% GT showed a significantly (P < 0.05) improved survival under simulated gastric conditions (pH 2.0, 2 h) and bile solution (3%, 2 h) when they were compared with microencapsulation without GT addition.</p> <p>Conclusions</p> <p>The results of this study suggest that green tea coencapsulated with <it>B. infantis</it> or <it>B. breve</it> exert a protective effect of bacteria during exposure to gastrointestinal conditions and refrigerated storage. For a health perspective, the results confirm the growing interest probiotic bacteria and the perceived benefit of increasing their numbers in the gastrointestinal tract by microencapsulation.</p>http://journal.chemistrycentral.com/content/6/1/61Green Tea<it>B. Infantis</it><it>B. Breve</it>MicroencapsulationGastrointestinal conditionsPolyphenols |
| spellingShingle | Vodnar Dan C Socaciu Carmen Green tea increases the survival yield of Bifidobacteria in simulated gastrointestinal environment and during refrigerated conditions Chemistry Central Journal Green Tea <it>B. Infantis</it> <it>B. Breve</it> Microencapsulation Gastrointestinal conditions Polyphenols |
| title | Green tea increases the survival yield of Bifidobacteria in simulated gastrointestinal environment and during refrigerated conditions |
| title_full | Green tea increases the survival yield of Bifidobacteria in simulated gastrointestinal environment and during refrigerated conditions |
| title_fullStr | Green tea increases the survival yield of Bifidobacteria in simulated gastrointestinal environment and during refrigerated conditions |
| title_full_unstemmed | Green tea increases the survival yield of Bifidobacteria in simulated gastrointestinal environment and during refrigerated conditions |
| title_short | Green tea increases the survival yield of Bifidobacteria in simulated gastrointestinal environment and during refrigerated conditions |
| title_sort | green tea increases the survival yield of bifidobacteria in simulated gastrointestinal environment and during refrigerated conditions |
| topic | Green Tea <it>B. Infantis</it> <it>B. Breve</it> Microencapsulation Gastrointestinal conditions Polyphenols |
| url | http://journal.chemistrycentral.com/content/6/1/61 |
| work_keys_str_mv | AT vodnardanc greenteaincreasesthesurvivalyieldofbifidobacteriainsimulatedgastrointestinalenvironmentandduringrefrigeratedconditions AT socaciucarmen greenteaincreasesthesurvivalyieldofbifidobacteriainsimulatedgastrointestinalenvironmentandduringrefrigeratedconditions |