Antibacterial activity of plant extracts against Streptococcus equi subsp. zooepidemicus isolates from guinea pigs with lymphadenitis in Ecuador
Lymphadenitis is a commonly occurring and contagious disease in guinea pigs caused by different pathogens, including Streptococcus sp., Staphylococcus sp., and Corynebacterium sp. This study aimed to characterize the bacteria isolated from pus extracted from abscessed mandibular lymph nodes of disea...
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Elsevier
2024-02-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402401257X |
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| author | Yadira F. Ordóñez Estefanía Miranda María Fernanda López Paola E. Ordóñez |
| author_facet | Yadira F. Ordóñez Estefanía Miranda María Fernanda López Paola E. Ordóñez |
| author_sort | Yadira F. Ordóñez |
| collection | DOAJ |
| description | Lymphadenitis is a commonly occurring and contagious disease in guinea pigs caused by different pathogens, including Streptococcus sp., Staphylococcus sp., and Corynebacterium sp. This study aimed to characterize the bacteria isolated from pus extracted from abscessed mandibular lymph nodes of diseased guinea pigs in Ecuador in 2019 and evaluate the in vitro antibacterial activity of the total extracts of three plant species. Isolates were recovered from three diseased guinea pigs with Lymphadenitis on a farm in Imbabura, Ecuador province. The bacteria were characterized through microbiological, biochemical, and molecular tests as Streptococcus equi subsp. zooepidemicus. Furthermore, the susceptibility of S. equi subsp. zooepidemicus to three plant extracts belonging to the Asteraceae family, Acmella ciliata, Bidens andicola, and Gazania splendens collected in Ecuador, were assessed in vitro by the microdilution method. Our data indicate that all the evaluated extracts showed activity, with a Minimum Inhibitory Concentration (MIC) of 22.50 mg/mL for Acmella ciliata, 11.25 mg/mL for Bidens andicola, and 5.60 mg/mL for Gazania splendens. Bidens andicola extract showed the highest efficacy with a % inhibition of 63.90 at the highest tested concentration (45 mg/mL). This is the first report on the bioactivity of these plant species against S. equi subsp. zooepidemicus. |
| first_indexed | 2024-03-08T00:11:25Z |
| format | Article |
| id | doaj.art-b34c3062427f41ca952e09dc63b14632 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T00:11:25Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj.art-b34c3062427f41ca952e09dc63b146322024-02-17T06:40:08ZengElsevierHeliyon2405-84402024-02-01103e25226Antibacterial activity of plant extracts against Streptococcus equi subsp. zooepidemicus isolates from guinea pigs with lymphadenitis in EcuadorYadira F. Ordóñez0Estefanía Miranda1María Fernanda López2Paola E. Ordóñez3Grupo de Investigación Productos Naturales Bioactivos, Escuela de Ciencias Agrícolas y Ambientales, Pontificia Universidad Católica del Ecuador-Ibarra, Av. Jorge Guzmán Rueda y Av. Padre Aurelio Espinosa Polit, 100112, Ibarra, Ecuador; Corresponding author.Escuela de Ciencias Agrícolas y Ambientales, Pontificia Universidad Católica del Ecuador-Ibarra, Av. Jorge Guzmán Rueda y Av. Padre Aurelio Espinosa Polit, 100112, Ibarra, EcuadorEscuela de Ciencias Agrícolas y Ambientales, Pontificia Universidad Católica del Ecuador-Ibarra, Av. Jorge Guzmán Rueda y Av. Padre Aurelio Espinosa Polit, 100112, Ibarra, EcuadorGrupo de Investigación Aplicada en Materiales y Procesos (GIAMP), School of Chemical Sciences and Engineering, Yachay Tech University, Hda. San José s/n y Proyecto Yachay, 100119, Urcuquí, Ecuador; Corresponding author.Lymphadenitis is a commonly occurring and contagious disease in guinea pigs caused by different pathogens, including Streptococcus sp., Staphylococcus sp., and Corynebacterium sp. This study aimed to characterize the bacteria isolated from pus extracted from abscessed mandibular lymph nodes of diseased guinea pigs in Ecuador in 2019 and evaluate the in vitro antibacterial activity of the total extracts of three plant species. Isolates were recovered from three diseased guinea pigs with Lymphadenitis on a farm in Imbabura, Ecuador province. The bacteria were characterized through microbiological, biochemical, and molecular tests as Streptococcus equi subsp. zooepidemicus. Furthermore, the susceptibility of S. equi subsp. zooepidemicus to three plant extracts belonging to the Asteraceae family, Acmella ciliata, Bidens andicola, and Gazania splendens collected in Ecuador, were assessed in vitro by the microdilution method. Our data indicate that all the evaluated extracts showed activity, with a Minimum Inhibitory Concentration (MIC) of 22.50 mg/mL for Acmella ciliata, 11.25 mg/mL for Bidens andicola, and 5.60 mg/mL for Gazania splendens. Bidens andicola extract showed the highest efficacy with a % inhibition of 63.90 at the highest tested concentration (45 mg/mL). This is the first report on the bioactivity of these plant species against S. equi subsp. zooepidemicus.http://www.sciencedirect.com/science/article/pii/S240584402401257XPlant extractsAsteraceae familyStreptococcus equi subsp. zooepidemicusLymphadenitisGuinea pigs |
| spellingShingle | Yadira F. Ordóñez Estefanía Miranda María Fernanda López Paola E. Ordóñez Antibacterial activity of plant extracts against Streptococcus equi subsp. zooepidemicus isolates from guinea pigs with lymphadenitis in Ecuador Heliyon Plant extracts Asteraceae family Streptococcus equi subsp. zooepidemicus Lymphadenitis Guinea pigs |
| title | Antibacterial activity of plant extracts against Streptococcus equi subsp. zooepidemicus isolates from guinea pigs with lymphadenitis in Ecuador |
| title_full | Antibacterial activity of plant extracts against Streptococcus equi subsp. zooepidemicus isolates from guinea pigs with lymphadenitis in Ecuador |
| title_fullStr | Antibacterial activity of plant extracts against Streptococcus equi subsp. zooepidemicus isolates from guinea pigs with lymphadenitis in Ecuador |
| title_full_unstemmed | Antibacterial activity of plant extracts against Streptococcus equi subsp. zooepidemicus isolates from guinea pigs with lymphadenitis in Ecuador |
| title_short | Antibacterial activity of plant extracts against Streptococcus equi subsp. zooepidemicus isolates from guinea pigs with lymphadenitis in Ecuador |
| title_sort | antibacterial activity of plant extracts against streptococcus equi subsp zooepidemicus isolates from guinea pigs with lymphadenitis in ecuador |
| topic | Plant extracts Asteraceae family Streptococcus equi subsp. zooepidemicus Lymphadenitis Guinea pigs |
| url | http://www.sciencedirect.com/science/article/pii/S240584402401257X |
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