Preparation and optimization of poly (lactic-co-glycolic acid) rod-shaped particles in nano size range for paclitaxel delivery
Nanoparticle shape has been acknowledged as an important design parameter due to its influence on nanoparticle interaction with biological systems. However, there is lacking of simple and scalable preparation technique for drug loaded non-spherical polymeric nanoparticles for a long time, thus hinde...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1103990/full |
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| author | Mengyao Xu Mengyao Xu Zuyue Liao Zuyue Liao Yang Liu Yang Liu Shiwei Guo Shiwei Guo Haiyang Hu Haiyang Hu Tao Chen Tao Chen Yuesong Wu Yuesong Wu Shengli Wan Shengli Wan Meiling Zhou Meiling Zhou Muhe Lu Shiluo Jiluo Lan Yao Xiaofeng Pu Shurong Wang Shurong Wang Qingze Fan Qingze Fan |
| author_facet | Mengyao Xu Mengyao Xu Zuyue Liao Zuyue Liao Yang Liu Yang Liu Shiwei Guo Shiwei Guo Haiyang Hu Haiyang Hu Tao Chen Tao Chen Yuesong Wu Yuesong Wu Shengli Wan Shengli Wan Meiling Zhou Meiling Zhou Muhe Lu Shiluo Jiluo Lan Yao Xiaofeng Pu Shurong Wang Shurong Wang Qingze Fan Qingze Fan |
| author_sort | Mengyao Xu |
| collection | DOAJ |
| description | Nanoparticle shape has been acknowledged as an important design parameter due to its influence on nanoparticle interaction with biological systems. However, there is lacking of simple and scalable preparation technique for drug loaded non-spherical polymeric nanoparticles for a long time, thus hindering the potential applications. Although our previous research has modified the traditional emulsion solvent evaporation technique by adding guest molecules to prepare non-spherical poly (lactic-co-glycolic acid) (PLGA) particles, it is difficult to obtain nano-sized rods with minor axis less than 200 nm, which may have great potential in cancer therapy. Herein, in present research, the two-step ESE method was used and optimized to prepare poly (lactic-co-glycolic acid) nanorods for paclitaxel delivery. Firstly, the single-factor experiment was used to screen the influence of multi-factors including type of guest molecules, concentration of guest molecules, emulsification method, surfactant concentration, oil volume, poly (lactic-co-glycolic acid) concentration on the size and shape to determine the range of variables; based on the above range, a multi-factor and multi-level orthogonal experiment was designed. The formula is evaluated by the rod fabrication yield and the aspect ratio of major axis to minor axis. The results showed that the yield of nanorods in the optimal formula was 99% and the aspect ratio was 5.35 ± 2.05 with the minor axis of 135.49 ± 72.66 nm, and major axis of 657.77 ± 307.63 nm. In addition, the anti-cancer drug paclitaxel was successfully encapsulated in PLGA nanorods by the same technique. Our results not only enrich the ESE technique for preparing small sized poly (lactic-co-glycolic acid) nanorods, but also envision the potential application of nanorods for targeted cancer therapy with the delivery of paclitaxel. |
| first_indexed | 2024-04-12T01:16:49Z |
| format | Article |
| id | doaj.art-b34cd3d1fa75482ab589fdbfbd789601 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-12T01:16:49Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-b34cd3d1fa75482ab589fdbfbd7896012022-12-22T03:53:57ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-12-011010.3389/fbioe.2022.11039901103990Preparation and optimization of poly (lactic-co-glycolic acid) rod-shaped particles in nano size range for paclitaxel deliveryMengyao Xu0Mengyao Xu1Zuyue Liao2Zuyue Liao3Yang Liu4Yang Liu5Shiwei Guo6Shiwei Guo7Haiyang Hu8Haiyang Hu9Tao Chen10Tao Chen11Yuesong Wu12Yuesong Wu13Shengli Wan14Shengli Wan15Meiling Zhou16Meiling Zhou17Muhe Lu18Shiluo Jiluo19Lan Yao20Xiaofeng Pu21Shurong Wang22Shurong Wang23Qingze Fan24Qingze Fan25Department of Pharmacy, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Clinical Pharmacy, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Pharmacy, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Clinical Pharmacy, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Pharmacy, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Clinical Pharmacy, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Pharmacy, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Clinical Pharmacy, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Pharmacy, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Clinical Pharmacy, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Pharmacy, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Clinical Pharmacy, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Pharmacy, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Clinical Pharmacy, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Pharmacy, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Clinical Pharmacy, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Pharmacy, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Clinical Pharmacy, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Clinical Pharmacy, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Clinical Pharmacy, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Pharmacy, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Pharmacy, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Pharmacy, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Clinical Pharmacy, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Pharmacy, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Clinical Pharmacy, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, ChinaNanoparticle shape has been acknowledged as an important design parameter due to its influence on nanoparticle interaction with biological systems. However, there is lacking of simple and scalable preparation technique for drug loaded non-spherical polymeric nanoparticles for a long time, thus hindering the potential applications. Although our previous research has modified the traditional emulsion solvent evaporation technique by adding guest molecules to prepare non-spherical poly (lactic-co-glycolic acid) (PLGA) particles, it is difficult to obtain nano-sized rods with minor axis less than 200 nm, which may have great potential in cancer therapy. Herein, in present research, the two-step ESE method was used and optimized to prepare poly (lactic-co-glycolic acid) nanorods for paclitaxel delivery. Firstly, the single-factor experiment was used to screen the influence of multi-factors including type of guest molecules, concentration of guest molecules, emulsification method, surfactant concentration, oil volume, poly (lactic-co-glycolic acid) concentration on the size and shape to determine the range of variables; based on the above range, a multi-factor and multi-level orthogonal experiment was designed. The formula is evaluated by the rod fabrication yield and the aspect ratio of major axis to minor axis. The results showed that the yield of nanorods in the optimal formula was 99% and the aspect ratio was 5.35 ± 2.05 with the minor axis of 135.49 ± 72.66 nm, and major axis of 657.77 ± 307.63 nm. In addition, the anti-cancer drug paclitaxel was successfully encapsulated in PLGA nanorods by the same technique. Our results not only enrich the ESE technique for preparing small sized poly (lactic-co-glycolic acid) nanorods, but also envision the potential application of nanorods for targeted cancer therapy with the delivery of paclitaxel.https://www.frontiersin.org/articles/10.3389/fbioe.2022.1103990/fullnon-spherical polymeric nanoparticlerod shapepoly (lactic-co-glycolic) acidemulsion solvent evaporation methodorthogonal experiment |
| spellingShingle | Mengyao Xu Mengyao Xu Zuyue Liao Zuyue Liao Yang Liu Yang Liu Shiwei Guo Shiwei Guo Haiyang Hu Haiyang Hu Tao Chen Tao Chen Yuesong Wu Yuesong Wu Shengli Wan Shengli Wan Meiling Zhou Meiling Zhou Muhe Lu Shiluo Jiluo Lan Yao Xiaofeng Pu Shurong Wang Shurong Wang Qingze Fan Qingze Fan Preparation and optimization of poly (lactic-co-glycolic acid) rod-shaped particles in nano size range for paclitaxel delivery Frontiers in Bioengineering and Biotechnology non-spherical polymeric nanoparticle rod shape poly (lactic-co-glycolic) acid emulsion solvent evaporation method orthogonal experiment |
| title | Preparation and optimization of poly (lactic-co-glycolic acid) rod-shaped particles in nano size range for paclitaxel delivery |
| title_full | Preparation and optimization of poly (lactic-co-glycolic acid) rod-shaped particles in nano size range for paclitaxel delivery |
| title_fullStr | Preparation and optimization of poly (lactic-co-glycolic acid) rod-shaped particles in nano size range for paclitaxel delivery |
| title_full_unstemmed | Preparation and optimization of poly (lactic-co-glycolic acid) rod-shaped particles in nano size range for paclitaxel delivery |
| title_short | Preparation and optimization of poly (lactic-co-glycolic acid) rod-shaped particles in nano size range for paclitaxel delivery |
| title_sort | preparation and optimization of poly lactic co glycolic acid rod shaped particles in nano size range for paclitaxel delivery |
| topic | non-spherical polymeric nanoparticle rod shape poly (lactic-co-glycolic) acid emulsion solvent evaporation method orthogonal experiment |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1103990/full |
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