Algicidal substances of Brevibacillus laterosporus and their effect on red tide organisms
Herein, the algicidal effects of Brevibacillus laterosporus on typical red tide organisms were investigated. Through comparative analysis of the fermentation solution, sterile filtrate and bacterial body, it was found that this strain mainly exerts its algicidal function by secreting algicidal subst...
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| Format: | Article |
| Idioma: | English |
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Frontiers Media S.A.
2023-03-01
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| Col·lecció: | Frontiers in Marine Science |
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| Accés en línia: | https://www.frontiersin.org/articles/10.3389/fmars.2023.1160585/full |
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| author | Shanshan Liu Shanshan Liu Shanshan Liu Shanshan Liu Zhiming Yu Zhiming Yu Zhiming Yu Zhiming Yu Zaixing Wu Zaixing Wu Zaixing Wu Xihua Cao Xihua Cao Xihua Cao Xihua Cao Ruihong Cheng Ruihong Cheng Ruihong Cheng Ruihong Cheng Xiuxian Song Xiuxian Song Xiuxian Song Xiuxian Song |
| author_facet | Shanshan Liu Shanshan Liu Shanshan Liu Shanshan Liu Zhiming Yu Zhiming Yu Zhiming Yu Zhiming Yu Zaixing Wu Zaixing Wu Zaixing Wu Xihua Cao Xihua Cao Xihua Cao Xihua Cao Ruihong Cheng Ruihong Cheng Ruihong Cheng Ruihong Cheng Xiuxian Song Xiuxian Song Xiuxian Song Xiuxian Song |
| author_sort | Shanshan Liu |
| collection | DOAJ |
| description | Herein, the algicidal effects of Brevibacillus laterosporus on typical red tide organisms were investigated. Through comparative analysis of the fermentation solution, sterile filtrate and bacterial body, it was found that this strain mainly exerts its algicidal function by secreting algicidal substances. In this paper, we established a method for extracting the algicidal substances of B. laterosporus and systematically investigated their features and effect on Heterosigma akashiwo. The results showed that the algicidal substances are a mixture of compounds all with molecular weights less than 3500 Da, with those below 100 Da producing 45% of the algicidal effect; these are more polar and best extracted with methanol. The algicidal substances are stable over a range of temperatures (-80.0 to 70.0°C) and pH values (4.0-8.0). The algicidal substances caused most H. akashiwo cells to rupture within 24 h. In the remaining cells, the algicidal substances activated the antioxidant system and reduced their metabolic activity, leading to apoptosis, as observed by cell crumbling and a reduction in membrane potential. The responses of different algal cell surface structures to the algicidal substances were also compared and analysed. It was concluded that these algicidal substances can act on the cell membrane and change its permeability, allowing entry of the algicidal substances to produce an algicidal effect. |
| first_indexed | 2024-04-09T20:16:48Z |
| format | Article |
| id | doaj.art-b34eecc39f624f50b6b901b79b294a74 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-09T20:16:48Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-b34eecc39f624f50b6b901b79b294a742023-03-31T07:02:28ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452023-03-011010.3389/fmars.2023.11605851160585Algicidal substances of Brevibacillus laterosporus and their effect on red tide organismsShanshan Liu0Shanshan Liu1Shanshan Liu2Shanshan Liu3Zhiming Yu4Zhiming Yu5Zhiming Yu6Zhiming Yu7Zaixing Wu8Zaixing Wu9Zaixing Wu10Xihua Cao11Xihua Cao12Xihua Cao13Xihua Cao14Ruihong Cheng15Ruihong Cheng16Ruihong Cheng17Ruihong Cheng18Xiuxian Song19Xiuxian Song20Xiuxian Song21Xiuxian Song22Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory of Marine Ecology and Environmental Science, Laoshan Laboratory, Qingdao, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaKey Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory of Marine Ecology and Environmental Science, Laoshan Laboratory, Qingdao, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaKey Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory of Marine Ecology and Environmental Science, Laoshan Laboratory, Qingdao, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaKey Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory of Marine Ecology and Environmental Science, Laoshan Laboratory, Qingdao, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaKey Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory of Marine Ecology and Environmental Science, Laoshan Laboratory, Qingdao, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaKey Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory of Marine Ecology and Environmental Science, Laoshan Laboratory, Qingdao, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaCenter for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaHerein, the algicidal effects of Brevibacillus laterosporus on typical red tide organisms were investigated. Through comparative analysis of the fermentation solution, sterile filtrate and bacterial body, it was found that this strain mainly exerts its algicidal function by secreting algicidal substances. In this paper, we established a method for extracting the algicidal substances of B. laterosporus and systematically investigated their features and effect on Heterosigma akashiwo. The results showed that the algicidal substances are a mixture of compounds all with molecular weights less than 3500 Da, with those below 100 Da producing 45% of the algicidal effect; these are more polar and best extracted with methanol. The algicidal substances are stable over a range of temperatures (-80.0 to 70.0°C) and pH values (4.0-8.0). The algicidal substances caused most H. akashiwo cells to rupture within 24 h. In the remaining cells, the algicidal substances activated the antioxidant system and reduced their metabolic activity, leading to apoptosis, as observed by cell crumbling and a reduction in membrane potential. The responses of different algal cell surface structures to the algicidal substances were also compared and analysed. It was concluded that these algicidal substances can act on the cell membrane and change its permeability, allowing entry of the algicidal substances to produce an algicidal effect.https://www.frontiersin.org/articles/10.3389/fmars.2023.1160585/fullBrevibacillus laterosporusred tide organismsalgicidal mechanismsalgicidal substancesalgicidal bacteria |
| spellingShingle | Shanshan Liu Shanshan Liu Shanshan Liu Shanshan Liu Zhiming Yu Zhiming Yu Zhiming Yu Zhiming Yu Zaixing Wu Zaixing Wu Zaixing Wu Xihua Cao Xihua Cao Xihua Cao Xihua Cao Ruihong Cheng Ruihong Cheng Ruihong Cheng Ruihong Cheng Xiuxian Song Xiuxian Song Xiuxian Song Xiuxian Song Algicidal substances of Brevibacillus laterosporus and their effect on red tide organisms Frontiers in Marine Science Brevibacillus laterosporus red tide organisms algicidal mechanisms algicidal substances algicidal bacteria |
| title | Algicidal substances of Brevibacillus laterosporus and their effect on red tide organisms |
| title_full | Algicidal substances of Brevibacillus laterosporus and their effect on red tide organisms |
| title_fullStr | Algicidal substances of Brevibacillus laterosporus and their effect on red tide organisms |
| title_full_unstemmed | Algicidal substances of Brevibacillus laterosporus and their effect on red tide organisms |
| title_short | Algicidal substances of Brevibacillus laterosporus and their effect on red tide organisms |
| title_sort | algicidal substances of brevibacillus laterosporus and their effect on red tide organisms |
| topic | Brevibacillus laterosporus red tide organisms algicidal mechanisms algicidal substances algicidal bacteria |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2023.1160585/full |
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