Species Identification of Red Deer (<i>Cervus elaphus</i>), Roe Deer (<i>Capreolus capreolus</i>), and Water Deer (<i>Hydropotes inermis</i>) Using Capillary Electrophoresis-Based Multiplex PCR
To provide consumers correct information on meat species, specific and sensitive detection methods are needed. Thus, we developed a capillary electrophoresis-based multiplex PCR assay to simultaneously detect red deer (<i>Cervus elaphus</i>), roe deer (<i>Capreolus capreolus</i&...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/8/982 |
| _version_ | 1827712472219385856 |
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| author | Mi-Ju Kim Yu-Min Lee Seung-Man Suh Hae-Yeong Kim |
| author_facet | Mi-Ju Kim Yu-Min Lee Seung-Man Suh Hae-Yeong Kim |
| author_sort | Mi-Ju Kim |
| collection | DOAJ |
| description | To provide consumers correct information on meat species, specific and sensitive detection methods are needed. Thus, we developed a capillary electrophoresis-based multiplex PCR assay to simultaneously detect red deer (<i>Cervus elaphus</i>), roe deer (<i>Capreolus capreolus</i>), and water deer (<i>Hydropotes inermis</i>). Specific primer sets for these three species were newly designed. Each primer set only amplified target species without any reactivity against non-target species. To identify multiple targets in a single reaction, multiplex PCR was optimized and combined with capillary electrophoresis to increase resolution and accuracy for the detection of multiple targets. The detection levels of this assay were 0.1 pg for red deer and roe deer and 1 pg for water deer. In addition, its applicability was demonstrated using various concentrations of meat DNA mixtures. Consequently, as low as 0.1% of the target species was detectable using the developed method. This capillary electrophoresis-based multiplex PCR assay for simultaneous detection of three types of deer meat could authenticate deer species labeled on products, thus protecting consumers from meat adulteration. |
| first_indexed | 2024-03-10T18:15:17Z |
| format | Article |
| id | doaj.art-b351917901d74b479fc64ee8b1189e27 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T18:15:17Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-b351917901d74b479fc64ee8b1189e272023-11-20T07:45:51ZengMDPI AGFoods2304-81582020-07-019898210.3390/foods9080982Species Identification of Red Deer (<i>Cervus elaphus</i>), Roe Deer (<i>Capreolus capreolus</i>), and Water Deer (<i>Hydropotes inermis</i>) Using Capillary Electrophoresis-Based Multiplex PCRMi-Ju Kim0Yu-Min Lee1Seung-Man Suh2Hae-Yeong Kim3Institute of Life Sciences & Resources and Department of Food Science & Biotechnology, Kyung Hee University, Yongin 17104, KoreaInstitute of Life Sciences & Resources and Department of Food Science & Biotechnology, Kyung Hee University, Yongin 17104, KoreaInstitute of Life Sciences & Resources and Department of Food Science & Biotechnology, Kyung Hee University, Yongin 17104, KoreaInstitute of Life Sciences & Resources and Department of Food Science & Biotechnology, Kyung Hee University, Yongin 17104, KoreaTo provide consumers correct information on meat species, specific and sensitive detection methods are needed. Thus, we developed a capillary electrophoresis-based multiplex PCR assay to simultaneously detect red deer (<i>Cervus elaphus</i>), roe deer (<i>Capreolus capreolus</i>), and water deer (<i>Hydropotes inermis</i>). Specific primer sets for these three species were newly designed. Each primer set only amplified target species without any reactivity against non-target species. To identify multiple targets in a single reaction, multiplex PCR was optimized and combined with capillary electrophoresis to increase resolution and accuracy for the detection of multiple targets. The detection levels of this assay were 0.1 pg for red deer and roe deer and 1 pg for water deer. In addition, its applicability was demonstrated using various concentrations of meat DNA mixtures. Consequently, as low as 0.1% of the target species was detectable using the developed method. This capillary electrophoresis-based multiplex PCR assay for simultaneous detection of three types of deer meat could authenticate deer species labeled on products, thus protecting consumers from meat adulteration.https://www.mdpi.com/2304-8158/9/8/982red deerroe deerwater deermultiplex PCRcapillary electrophoresis |
| spellingShingle | Mi-Ju Kim Yu-Min Lee Seung-Man Suh Hae-Yeong Kim Species Identification of Red Deer (<i>Cervus elaphus</i>), Roe Deer (<i>Capreolus capreolus</i>), and Water Deer (<i>Hydropotes inermis</i>) Using Capillary Electrophoresis-Based Multiplex PCR Foods red deer roe deer water deer multiplex PCR capillary electrophoresis |
| title | Species Identification of Red Deer (<i>Cervus elaphus</i>), Roe Deer (<i>Capreolus capreolus</i>), and Water Deer (<i>Hydropotes inermis</i>) Using Capillary Electrophoresis-Based Multiplex PCR |
| title_full | Species Identification of Red Deer (<i>Cervus elaphus</i>), Roe Deer (<i>Capreolus capreolus</i>), and Water Deer (<i>Hydropotes inermis</i>) Using Capillary Electrophoresis-Based Multiplex PCR |
| title_fullStr | Species Identification of Red Deer (<i>Cervus elaphus</i>), Roe Deer (<i>Capreolus capreolus</i>), and Water Deer (<i>Hydropotes inermis</i>) Using Capillary Electrophoresis-Based Multiplex PCR |
| title_full_unstemmed | Species Identification of Red Deer (<i>Cervus elaphus</i>), Roe Deer (<i>Capreolus capreolus</i>), and Water Deer (<i>Hydropotes inermis</i>) Using Capillary Electrophoresis-Based Multiplex PCR |
| title_short | Species Identification of Red Deer (<i>Cervus elaphus</i>), Roe Deer (<i>Capreolus capreolus</i>), and Water Deer (<i>Hydropotes inermis</i>) Using Capillary Electrophoresis-Based Multiplex PCR |
| title_sort | species identification of red deer i cervus elaphus i roe deer i capreolus capreolus i and water deer i hydropotes inermis i using capillary electrophoresis based multiplex pcr |
| topic | red deer roe deer water deer multiplex PCR capillary electrophoresis |
| url | https://www.mdpi.com/2304-8158/9/8/982 |
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